Sunil Taak

Impact of Novel Purified Fatty Acid Esterases on the Biochemical Properties and Shelf Life of Finger Millet

Sunil Taak, L.K. Chugh, Nisha, Kavita Dhaka & Arjun Ram · Journal of Advances in Biology & Biotechnology · 2025

This study focused on isolating fatty acid esterase from finger millet flour and examining its physicochemical and kinetic characteristics. The enzyme was partially purified from fresh flour of the GPU 28 finger millet variety using ammonium sulphate precipitation and dialysis, f...

Open access Research Article 10.9734/jabb/2025/v28i123390

Purification and Comparative Insight into Physico-chemical and Kinetic Properties of Fatty Acid Esterases Novel Isoforms from Pearl Millet and their Effect on Shelf Life

Sunil Taak, L.K. Chugh, Nisha, Kavita Dhaka & Arjun Ram · Journal of Advances in Biology & Biotechnology · 2025

The current study focused on isolating and characterising fatty acid esterase from pearl millet, specifically comparing enzymes derived from F1 seeds and F2 grains. Purification was achieved through ammonium sulphate precipitation, dialysis, and gel filtration chromatography. Sep...

Open access Research Article 10.9734/jabb/2025/v28i123363

Insight Role of Fatty Acid Esterases Kinetics in Shelf Life of Millet Flour

Sunil Taak · Asian Journal of Research in Biochemistry · 2025

Esterases are lipolytic enzymes that use a water-based process known as hydrolysis to separate esters into an acid and an alcohol. Fatty acid esterases were purified through a series of steps, including ammonium sulfate precipitation, ion-exchange chromatography, using size exclu...

Open access Research Article 10.9734/ajrb/2025/v15i6453