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Research Article Open access CC BY 4.0

A Review on Plant Tissue Culture

Shivani Thakur, Shruti, Sohin Hashmi, Saket Mishra, Shashi Kant Ekka, Akhilesh Kushwaha, Reena Kujur

Asian Journal of Biology · pp. 14–18 · Published 15 Feb 2024

10.9734/ajob/2024/v20i2387

Abstract

Plant tissue and cell culture involve the aseptic cultivation and propagation of plant cells, tissues, and organs in a meticulously controlled laboratory setting. This innovative method harnesses the potential of nutrient-rich media to efficiently replicate plant cells on a large scale, resulting in the rapid production of mature and disease-free plants. The cornerstone of commercial technology in this field is micropropagation, a process that achieves swift proliferation from minute plant cuttings, axillary buds, and, to a limited extent, from somatic embryos and cell clumps in suspension cultures and bioreactors. Micropropagation, a pivotal aspect of plant tissue and cell culture, holds immense value in generating high-quality and consistent planting materials. These materials find applications across diverse fields, including molecular genetic engineering, plant breeding, horticulture production, and environmental preservation. The process of micropopagation unfolds through several distinct stages, including propagation, subculture of explants for proliferation, shooting and rooting, and hardening. These stages collectively form a universal framework for large-scale multiplication of plants. This technique plays a critical role in overcoming the limitations of traditional plant propagation methods. By allowing for controlled and accelerated growth in a laboratory environment, plant tissue and cell culture contribute to the efficient production of disease-resistant and genetically uniform plant materials. This not only supports advancements in various scientific disciplines but also addresses practical needs in agriculture, horticulture, and environmental conservation. The continuous refinement and application of plant tissue and cell culture methods underscore their significance in meeting the growing demands for sustainable and high-quality plant materials across diverse sectors.

Micropagation environment multiplication of plants

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