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Research Article Open access CC BY 3.0

Acute and Sub-acute Toxicity Studies of Ethanol Seed Extract of Raphia hookeri on Swiss Albino Rats

G. O. Mbaka, S. O. Ogbonnia, F. O. Awoyemi

Journal of Pharmaceutical Research International · pp. 1196–1208 · Published 5 May 2014

10.9734/BJPR/2014/9330

Abstract

Aim: To evaluate the acute and sub-acute toxicities of Raphia hookeri (Rh) seed hydroethanolic extract on experimental animals. Materials and Methods: Acute toxicity study was evaluated on Swiss albino mice of both sexes. Administration of a single dose of 4000mg/kg of Rh seed extract by gavages to five mice showed no mortality, hence, its 1/20th dose was used as the highest therapeutic dose. The intra-peritoneal administration produced dose dependent mortality with median lethal dose (LD50) of approximately 323.6mg/kg body weight (bwt). In sub-acute toxicity study, Wistar rats received daily administration of the extract in the dose range of 50 to 200mg/kgbwt for 30 days. The effects on biochemical, histological and haematological parameters were evaluated. Results: The animals exhibited dose dependent body weight changes. There were some organs weight gains with the exception of the liver and testes which showed comparably lower weight compared to the control. There was a significant (p<0.05) increase in total protein, aspartate aminotransferase (AST) and albumin levels compared to the control while bilirubin and alanine aminotransferase (ALT) levels decreased appreciably at the highest extract dose. The urea level decreased while the creatinine level increased in dose dependent manner. In lipid profile study, total cholesterol, triglycerides and low density lipoprotein cholesterol (LDL-cholesterol) levels showed significant (p<0.05) decrease in value. There was significant (p<0.05) increase in high density lipoprotein cholesterol (HDL-cholesterol). Marked decrease in red blood cells, haemoglobin and haematocrit occurred. The white blood cells also decreased while neutrophil and lymphocytes increased appreciably. The extract caused marked deleterious effect on the testes leading to drastic reduction in sperm cells. Conclusion: The extract caused undesirable effect on the male reproductive organ of the animals making it unsafe for consumption by males of reproductive age.

Raphia hookeri acute toxicity sub-acute toxicity tissue histology.

References (19)

  1. 1 Phenol-hypochlorite reaction for determination of ammonia [DOI]
  2. 2 Should we be concerned about herbal remedies [DOI]
  3. 3 Effect of prolonged vigabatrin treatment on hematological and biochemical parameters in plasma, liver and kidney of Swiss albino mice [DOI]
  4. 4 Hypoglycemic, hypolipidemic and antioxidant properties of combination ofCurcumin fromCurcuma longa, Linn, and partially purified product fromAbroma augusta, Linn. in streptozotocin induced diabetes [DOI]
  5. 5 Anti-inflammatory activities of the methanol extracts and an isolated furanoditerpene constituent of Sphenocentrum jollyanum Pierre (Menispermaceae) [DOI]
  6. 6 Oral Acute and Subacute Toxicity Studies with Kalpaamruthaa, a Modified Indigenous Preparation, on Rats [DOI]
  7. 7 Clinical Chemistry and Metabolic Medicine, 7th edn. [DOI]
  8. 8 The use of health foods, spices and other botanicals in the Sikh community in London [DOI]
  9. 9 Assessing plasma lipid levels, body weight, and hepatic and renal toxicity following chronic oral administration of a water soluble phytostanol compound, FM-VP4, to gerbils.
  10. 10 Acute And Subchronic Toxicity Of Anacardium occidentale Linn (Anacardiaceae) Leaves Hexane Extract In Mice. [DOI]
  11. 11 Evaluation of hypoglycaemic and hypolipidaemic effects of aqueous ethanolic extracts of Treculia africana Decne and Bryophyllum pinnatum,/i> Lam. and their mixture on streptozotocin (STZ)-induced diabetic rats [DOI]
  12. 12 Evaluation of the anti-oxidant and anti-angiogenic effects of Sphenocentrum Jollyanum Pierre [DOI]
  13. 13 Antimicrobial evaluation, acute and subchronic toxicity studies of Leone Bitters, a Nigerian polyherbal formulation, in rodents. [DOI]
  14. 14 Vitamin b12 supplementation: effects on some biochemical and haematological indices of rats on phenytoin administration [DOI]
  15. 15 Effect of Raphia hookeri Seed Extract on Blood Glucose, Glycosylated Haemoglobin and Lipid Profile of Alloxan Induced Diabetic Rats [DOI]
  16. 16 Evaluation of Haematinic Activity and Subchronic Toxicity of Sphenocentrum jollyanum (Menispermaceae) Seed Oil [DOI]
  17. 17 Safety Evaluation of Prolonged Administration of Stresroak® in Grower Cockerels [DOI]
  18. 18 Evaluation of Microbial Purity and Acute and Sub-acute Toxicities of a Nigerian Commercial Polyherbal Formulation Used in the Treatment of Diabetes Mellitus [DOI]
  19. 19 Some Morphological and Chemical Characteristics of Developing Fruits ofRaphia hookeri [DOI]

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