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Acute and Sub-chronic Toxicity of Aqueous Extract of Roots of Khaya senegalensis (Desr.) A. Juss. in Mice and Rats Respectively

Rachael O. Folarin, Jamiu O. Omirinde, Taidinda T. Gilbert, Christopher Igbeneghu

Annual Research & Review in Biology · pp. 33–46 · Published 9 Nov 2023

10.9734/arrb/2023/v38i830601

Abstract

Aim: Khaya senegalensis is one of the key medicinal plants used discretionarily in traditional medicine as remedies to several health conditions. This study aimed to establish the safety of Khaya senegalensis root aqueous extract in experimental animals with the purpose of optimizing its therapeutic value.  Methodology: A total of 74 animals (20 rats and 54 mice) were randomly assigned into two main groups based on toxicity plan; acute and sub-chronic toxicity. Mice were divided into 9 groups (6 per group) for the acute toxicity study while rats were divided into 4 groups (5 per group) for sub-chronic toxicity assessment.  Results: The acute concentrations of the extract in mice induced dose-dependent clinical signs severities such as: twitching, increase rate of respiration, sedation, abdominal muscle contractions and increased motor activity. The lethal dose 50 value of the extract was estimated as 320mg/ kg body. The sub-chronic concentrated grades in the rats especially the higher doses elicited significantly increased serum liver enzymes values when compared to the control, while at low dose the values were comparable to that of the control. Also observed were the evidences of renal cellular pathology ranging from mild to severe tubular cell degeneration, tubular cell depletion and congestion of the renal cortex. The liver pathologies such as hepatic portal congestion, cytoplasmic vacuolations and nuclear degeneration were strikingly visible mostly at the higher doses. The lymphocyte and platelet counts were the only haematological parameters that increased significantly more particularly at low dose when compared with the control. Conclusion: This study has shown that Khaya senegalensis seems to be safe only at low doses. However, caution should be taking in its administration for therapeutic purposes especially when long-term usage is desired.

LD50 medicinal plant cellular pathology haematology Khaya senegalensis

References (31)

  1. 1 Natural Products as Sources of New Drugs from 1981 to 2014 [DOI]
  2. 2 Clinical Biochemistry of Domestic Animals [DOI]
  3. 3 Trees and Shrubs of the Sahel: Their Characteristics and Uses
  4. 4 Proteins, Proteomics, and the Dysproteinemias [DOI]
  5. 5 Toxicity and safety of medicinal plants
  6. 6 Safety of medicinal plants
  7. 7 Toxicity profile of ethanolic extract of Azadirachta indica stem bark in male Wistar rats [DOI]
  8. 8 Proximate and Phytochemical Composition of Moringa oleifera Leaves at Three Stages of Maturation
  9. 9 African Medicinal Plants [DOI]
  10. 10 Immunomodulatory effects of aqueous extract of Tridax procumbens in experimental animals [DOI]
  11. 11 African Medicinal Plants with Antidiabetic Potentials: A Review [DOI]
  12. 12 Oral citrulline as arginine precursor may be beneficial in sickle cell disease: early phase two results.
  13. 13 Effects of ethanolic leaf extract of Chrysophyllum albidum G. on biochemical and haematological parameters of albino Wistar rats
  14. 14 Effect of ethanolic extract of Khaya senegalensis on some biochemical parameters of rat kidney [DOI]
  15. 15 Antifungal limonoids from the fruits of Khaya senegalensis [DOI]
  16. 16 Acute and subchronic toxicological assessment of Byrsocarpus coccineus Schum. and Thonn. (Connaraceae) leaf aqueous extract [DOI]
  17. 17 Toxicological evaluation and hepatoprotective potential of Clerodendron glandulosum.Coleb leaf extract [DOI]
  18. 18 Phytochemical screening and antimicrobial efficacy of extracts from Khaya senegalensis against human pathogenic bacteria [DOI]
  19. 19 Evaluation of the Acute and Subchronic Toxicities of Ethanol Leaf Extract of Spathodea campanulata P. Beauv.
  20. 20 Effects of Aqueous Extract of Khaya senegalensis Stem Bark on Biochemical and Hematological Parameters in Rats [DOI]
  21. 21 In vitro propagation of an endangered medicinal timber species Khaya grandifoliola C. Dc. [DOI]
  22. 22 Effect of Aqueous Stem Bark Extract ofKhaya senegalensison Some Biochemical, Haematological, and Histopathological Parameters of Rats [DOI]
  23. 23 In vitro and in vivo anti-diabetic and anti-oxidant activities of methanolic leaf extracts of Ocimum canum. [DOI]
  24. 24 In vitro and in vivo antitrypanosomal efficacy of combination therapy of Anogeissus leiocarpus, Khaya senegalensis and potash [DOI]
  25. 25 Influence of processing cassava peels on the hydrogen cyanide concentration, nutritive value and performance of growing rabbits [DOI]
  26. 26 Mechanism of the Anti-inflammatory Activity of Khaya senegalensis A. Juss. (Meliaceae) [DOI]
  27. 27 Ethnobotanical survey of plants used by traditional health practitioners for treatment of schizophrenia spectrum disorders in Bandiagara, Mali, West Africa [DOI]
  28. 28 Acute and sub-chronic oral toxicity studies of the leaves aqueous extract of Clerodendrum umbellatum Poir. on mice [DOI]
  29. 29 Review of the antioxidant potential of African medicinal and food plants. [DOI]
  30. 30 Effect of Vitellaria paradoxa Stem Bark Ingestion on Kidney Functions in Wistar Rats [DOI]
  31. 31 Endogenous and exogenous factors modifying the activity of human liver cytochrome P-450 enzymes [DOI]

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