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Research Article Open access CC BY 4.0

An Overview of Methods of Extraction, Isolation and Characterization of Natural Medicinal Plant Products in Improved Traditional Medicine Research

Dobgima John Fonmboh, Ejoh Richard Abah, Tembe Estella Fokunang, Bayaga Herve, Gerald Ngo Teke, Ngono Mballa Rose, Njinkio Nono Borgia, Lovet Benyella Fokunang, Banin Nyuyki Andrew, Nubia Kaba, Ngameni Bathelemy, Fokunang Charles Ntungwen

Asian Journal of Research in Medical and Pharmaceutical Sciences · pp. 31–57 · Published 20 Nov 2020

10.9734/ajrimps/2020/v9i230152

Abstract

Herbal plants are very important in traditional community use and enrich our plant biodiversity and conservation. Natural products are vital substances of traditional knowledge systems in complementary and alternative medicine, nutraceutical, food supplements, and pharmaceutical bioactive metabolites of new chemical entities. Bioactive secondary metabolites from herbal plants of different forms are main sources and provide major opportunities for drug active pharmaceuticals due to the diverse flora and fauna biodiversity that produces the necessary available chemical diversity. There has been an increasing popularity in phytochemical research within the high through put (HTS) screening programs in search of lead. Phytochemicals of herbal extracts for traditional uses contain various types of bioactive metabolites of pharmaceutical and pharmacotherapeutic nature, and many phytomedicines for different therapeutic areas have been derived from herbal products. This paper is aimed at giving an insight into the extraction, isolation, and characterization of the rich medicinal plant biodiversity of potential pharmaceutical importance and the major drawbacks and challenges in the extraction, isolation, and characterization of phytochemicals in plant extracts. Phytochemicals in medicinal plants have been studied with more emphasis on the extraction process which is a vital stage in the analysis of bioactive compounds in medicinal plant research. The advantages and disadvantages of the different extraction methods is important to discuss following the regulatory guidelines and different pharmacopoeia. The analysis of bioactive molecules in herbal products involves the applications of various phytochemical screening methods, and the use of chromatographic techniques such as TLC and HPLC, including in some cases the non-chromatographic methods like Fourier Transform Infra-Red (FTIR), immunoassay. This paper has been motivated by the challenges faced by most pharmacy students in data mining of information on phytochemical screening and testing of biological activities in projects related to herbal plants research. This write up is also geared towards providing students with information on the preclinical drug discovery process towards the formulation of an improved traditional medicine/ phytomedicine.

Phytochemicals bioactive secondary metabolites plant extraction natural products.

References (39)

  1. 1 Phytochemical constituents of some Nigerian medicinal plants [DOI]
  2. 2 Extraction, Isolation And Characterization Of Bioactive Compounds From Plants’ Extracts [DOI]
  3. 3 Antimicrobial activity of some ethnomedicinal plants used by Paliyar tribe from Tamil Nadu, India [DOI]
  4. 4 In vitro Antimicrobial Activity and Phytochemical Analysis of Some Indian Medicinal Plants
  5. 5 Direct bioautography on thin-layer chromatograms as a method for detecting fungitoxic substances [DOI]
  6. 6 Bioactive compounds from natural resources against skin aging [DOI]
  7. 7 Separation procedures for naturally occurring antioxidant phytochemicals [DOI]
  8. 8 A bioautographic agar overlay method for the detection of antifungal compounds from higher plants [DOI]
  9. 9 The Okinawan Diet: Health Implications of a Low-Calorie, Nutrient-Dense, Antioxidant-Rich Dietary Pattern Low in Glycemic Load [DOI]
  10. 10 Different extraction methods of biologically active components from propolis: a preliminary study [DOI]
  11. 11 Effect of solvents in extracting polyphenols and antioxidants of selected raw vegetables [DOI]
  12. 12 Analysis of phenolic compounds in rhubarbs using liquid chromatography coupled with electrospray ionization mass spectrometry [DOI]
  13. 13 Multiple chromatographic fingerprinting and its application to the quality control of herbal medicines [DOI]
  14. 14 Antimicrobial effects of Indian medicinal plants against acne-inducing bacteria [DOI]
  15. 15 Identification and characterization of chemical compounds in different crude extracts from leaves of Omani neem [DOI]
  16. 16 Separation methods used for Scutellaria baicalensis active components [DOI]
  17. 17 Phytochemical Studies of Strychnos potatorum L.f.‐ A Medicinal Plant [DOI]
  18. 18 Extraction optimization of total triterpenoids from Jatropha curcas leaves using response surface methodology and evaluations of their antimicrobial and antioxidant capacities [DOI]
  19. 19 A Direct Bioautographic Tlc Assay for Compounds Possessing Antibacterial Activity [DOI]
  20. 20 On-line identification of phytochemical constituents in botanical extracts by combined high-performance liquid chromatographic–diode array detection–mass spectrometric techniques [DOI]
  21. 21 Spectrophotometric determination of unsaturated pyrrolizidine alkaloids [DOI]
  22. 22 A capsule review of recent studies on the application of mass spectrometry in the analysis of Chinese medicinal herbs [DOI]
  23. 23 Effect of extraction solvents and plant parts used on the antihyperlipidemic and antioxidant effects of Garcinia atroviridis: a comparative study [DOI]
  24. 24 Preparation of Samples of Plant Material for Chromatographic Analysis [DOI]
  25. 25 Cosmos Caudatus as a Potential Source of Polyphenolic Compounds: Optimisation of Oven Drying Conditions and Characterisation of Its Functional Properties [DOI]
  26. 26 Isolation of antibacterial pentahydroxy flavones from the seeds of Mimusops elengi Linn [DOI]
  27. 27 Preliminary Phytochemical Analysis of the Extracts of Psidium Leaves
  28. 28 Effect of Drying Methods on Metabolites Composition of Misai Kucing (Orthosiphon stamineus) Leaves [DOI]
  29. 29 Ultrasound-assisted extraction of phenolic compounds from Cratoxylum formosum ssp. formosum leaves using central composite design and evaluation of its protective ability against H2O2-induced cell death [DOI]
  30. 30 In Vivo. andIn Vitro. Toxicity Study ofGracilaria changii. [DOI]
  31. 31 Pressurized liquid extraction and GC‐MS analysis for simultaneous determination of seven components in Cinnamomum cassia and the effect of sample preparation [DOI]
  32. 32 Phytochemical and acute toxicity of ethanolic extract of Enantia chlorantha (oliv) stem bark in albino rats [DOI]
  33. 33 The Potential Pharmacological and Medicinal Properties of Neem (Azadirachta indica A. Juss) in the Drug Development of Phytomedicine [DOI]
  34. 34 Phytochemical Fingerprinting and In Vitro Bioassays of the Ethnomedicinal Fern Tectaria coadunata (J. Smith) C. Christensen from Central Nepal [DOI]
  35. 35 Rapid Multiresidue Screen for Alkaloids in Plant Material and Biological Samples [DOI]
  36. 36 Monoclonal antibodies against naturally occurring bioactive compounds [DOI]
  37. 37 Enzymatic pretreatment and microwave extraction of asiaticoside from Centella asiatica [DOI]
  38. 38 Pre-clinical in vitro investigation of the cytotoxic effect of Ficus species on hepatoma G2 cells using two standard toxicity assays [DOI]
  39. 39 In vitro Evaluation of the Interaction between Amoxicillin and Hydro Ethanolic Extracts of Annickia chlorantha [DOI]

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