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Research Article Open access CC BY 4.0

Analysis of Stress Responsive Genes in Capsicum for Salinity Responses

Vaibhav Kumar Maurya, R. Srinivasan, E. Nalini, N. Ramesh, K. M. Gothandam

Annual Research & Review in Biology · pp. 66–78 · Published 9 Dec 2014

10.9734/ARRB/2015/14107

Abstract

Aims: This study is an endeavor to gain proper understanding about salt tolerance mechanism in plants; an attempt was made to characterize the differential expression of stress responsive genes, sodium potassium content proline content in three capsicum cultivars having different salt sensitivity level. Place and Duration of Study: School of Bio Sciences and Technology, VIT University, Vellore of India between June 2013 to May 2014. Methodology: Capsicum cultivars (salt tolerant, salt moderate sensitive and salt susceptible) were treated with different concentration of NaCl such as 25mM, 50mM, 100mM, 150mM and 200mM. Gene expression studies under different salt treatment were done for the following genes: osmotic adjustment (CaPROX1), osmotin like protein (CaOSM1), aquaporin (CaPIP2), dehydrin responsive gene (CaDREBLP1), ring domain zinc finger protein gene (CaKR1), membrane protein (CaChi2), endoplasmic reticulum ubiquitine ligase (CaRMa1H1) and cell death repressor (CaBI1). Proline content and sodium and potassium ion content also measured. Results: The result indicated that genes CaDREBLP1, CaRMa1H1, CaKR1, CaOSM1 were up regulated while CaPROX1, CaPIP2 genes were down regulated under salt stress. But no significant difference was noticed in gene expression level of CaBI1 and CaChi 2 gene. Conclusion: The higher gene expression level of stress responsive genes viz. CaDREBLP1, CaRMa1H1, CaKR1, CaOSM1 may involved in different level of salt tolerance among selected cultivars. Thus differential transcript modulation of these genes in capsicum cultivars indicates their role lending the salt tolerance in salt tolerant cultivar than sensitive.

Stress responsive gene biotic abiotic osmolytes Salt tolerance transcript expression

References (53)

  1. 1 Rapid determination of free proline for water-stress studies [DOI]
  2. 2 Cell Signaling during Cold, Drought, and Salt Stress [DOI]
  3. 3 OsDREB genes in rice, Oryza sativa L., encode transcription activators that function in drought‐, high‐salt‐ and cold‐responsive gene expression [DOI]
  4. 4 The Arabidopsis NPR1 Gene That Controls Systemic Acquired Resistance Encodes a Novel Protein Containing Ankyrin Repeats [DOI]
  5. 5 Role of DREB transcription factors in abiotic and biotic stress tolerance in plants [DOI]
  6. 6 Role of DREBs in regulation of abiotic stress responses in plants [DOI]
  7. 7 Signal transduction during cold, salt, and drought stresses in plants [DOI]
  8. 8 Ubiquitin and ubiquitin-like proteins as multifunctional signals [DOI]
  9. 9 Mechanisms of High Salinity Tolerance in Plants [DOI]
  10. 10 Salt stress and phyto-biochemical responses of plants - a review [DOI]
  11. 11 Cell signaling under salt, water and cold stresses [DOI]
  12. 12 Whole Gene Family Expression and Drought Stress Regulation of Aquaporins [DOI]
  13. 13 ABA Levels and Sensitivity in Developing Wheat Embryos of Sprouting Resistant and Susceptible Cultivars [DOI]
  14. 14 Ubiquitin, Hormones and Biotic Stress in Plants [DOI]
  15. 15 An Expression Analysis of a Gene Family Encoding Plasma Membrane Aquaporins in Response to Abiotic Stresses in Arabidopsis Thaliana [DOI]
  16. 16 Antisense suppression of proline degradation improves tolerance to freezing and salinity in Arabidopsis thaliana [DOI]
  17. 17 Abiotic stress tolerance mediated by protein ubiquitination [DOI]
  18. 18 NaCl effects on proline metabolism in rice ( Oryza sativa ) seedlings [DOI]
  19. 19 The ubiquitin–proteasome system: central modifier of plant signalling [DOI]
  20. 20 Essential Role of Tissue-Specific Proline Synthesis and Catabolism in Growth and Redox Balance at Low Water Potential [DOI]
  21. 21 Enhancement of Oxidative Stress Tolerance in Transgenic Tobacco Plants Overproducing Fe-Superoxide Dismutase in Chloroplasts [DOI]
  22. 22 PIP1 Plasma Membrane Aquaporins in Tobacco [DOI]
  23. 23 A Recessive Arabidopsis Mutant That Grows Photoautotrophically under Salt Stress Shows Enhanced Active Oxygen Detoxification [DOI]
  24. 24 Molecular and Physiological Responses to Abscisic Acid and Salts in Roots of Salt-Sensitive and Salt-Tolerant Indica Rice Varieties [DOI]
  25. 25 The ethylene-, jasmonate-, abscisic acid- and NaCl-responsive tomato transcription factor JERF1 modulates expression of GCC box-containing genes and salt tolerance in tobacco [DOI]
  26. 26 Mammalian Bax-induced plant cell death can be down-regulated by overexpression of Arabidopsis Bax Inhibitor-1 ( AtBI-1 ) [DOI]
  27. 27 GmDREB2A;2, a Canonical DEHYDRATION-RESPONSIVE ELEMENT-BINDING PROTEIN2-Type Transcription Factor in Soybean, Is Posttranslationally Regulated and Mediates Dehydration-Responsive Element-Dependent Gene Expression [DOI]
  28. 28 Regulation of Transpiration to Improve Crop Water Use [DOI]
  29. 29 Drought tolerance through biotechnology: improving translation from the laboratory to farmers’ fields [DOI]
  30. 30 Arabidopsis Acyl-CoA-Binding Protein ACBP2 Interacts With an Ethylene-Responsive Element-Binding Protein, AtEBP, via its Ankyrin Repeats [DOI]
  31. 31 Ornithine‐delta‐aminotransferase and proline dehydrogenase genes play a role in non‐host disease resistance by regulating pyrroline‐5‐carboxylate metabolism‐induced hypersensitive response [DOI]
  32. 32 A gene family encoding RING finger proteins in rice: their expansion, expression diversity, and co-expressed genes [DOI]
  33. 33 Overexpression of barley BAX inhibitor 1 induces breakdown of mlo -mediated penetration resistance to Blumeria graminis [DOI]
  34. 34 Altered Levels of Proline Dehydrogenase Cause Hypersensitivity to Proline and Its Analogs in Arabidopsis [DOI]
  35. 35 Role of abscisic acid in chilling tolerance of rice (Oryza sativa L.) seedlings. [DOI]
  36. 36 Recent advance in the study of caspase‐like proteases and Bax inhibitor‐1 in plants: their possible roles as regulator of programmed cell death [DOI]
  37. 37 Overexpression of tobacco osmotin (Tbosm) in soybean conferred resistance to salinity stress and fungal infections [DOI]
  38. 38 Genetic engineering for abiotic stress resistance in crop plants [DOI]
  39. 39 Overexpression of a chrysanthemum transcription factor gene, DgWRKY3, in tobacco enhances tolerance to salt stress [DOI]
  40. 40 Promoter activation of pepper class II basic chitinase gene, CAChi2, and enhanced bacterial disease resistance and osmotic stress tolerance in the CAChi2-overexpressing Arabidopsis [DOI]
  41. 41 A BAX inhibitor-1 gene in Capsicum annuum is induced under various abiotic stresses and endows multi-tolerance in transgenic tobacco [DOI]
  42. 42 Expression analysis of RING zinc finger genes from Triticum aestivum and identification of TaRZF70 that contains four RING-H2 domains and differentially responds to water deficit between leaf and root [DOI]
  43. 43 Role of abscisic acid in chilling tolerance of rice (Oryza sativa L.) seedlings. I. Endogenous abscisic acid levels [DOI]
  44. 44 Ion balance in tomato cultivars differing in salt tolerance. I. Sodium and potassium accumulation and fluxes under moderate salinity [DOI]
  45. 45 Pepper gene encoding a basic class II chitinase is inducible by pathogen and ethephon [DOI]
  46. 46 Pepper osmotin-like protein 1 (CaOSM1) is an essential component for defense response, cell death, and oxidative burst in plants [DOI]
  47. 47 Characterization of a Stress-Responsive Ankyrin Repeat-Containing Zinc Finger Protein of Capsicum annuum (CaKR1) [DOI]
  48. 48 Ectopic Overexpression of SsCBF1, a CRT/DRE-Binding Factor from the Nightshade Plant Solanum lycopersicoides, Confers Freezing and Salt Tolerance in Transgenic Arabidopsis [DOI]
  49. 49 Engineering of betaine biosynthesis and transport for abiotic stress tolerance in plants [DOI]
  50. 50 Salt tolerance of six varieties of chillies
  51. 51 Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance [DOI]
  52. 52 Cold tolerance in Osmotin transgenic tomato (Solanum lycopersicum L.) is associated with modulation in transcript abundance of stress responsive genes [DOI]
  53. 53 Constitutive expression of CaRma1H1, a hot pepper ER-localized RING E3 ubiquitin ligase, increases tolerance to drought and salt stresses in transgenic tomato plants [DOI]

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