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Research Article Open access CC BY 4.0

Analysis of the Influence of Zn Excess in the Pineal Gland by Total Reflection X-ray Fluorescence

Correa-Gillieron, Elenice Maria, Barbosa, Renata Faria, Ferezin-Pinto, Caroline, Martinez, Ana Maria Blanco

Asian Journal of Biology · pp. 9–26 · Published 29 Dec 2022

10.9734/ajob/2022/v16i4307

Abstract

The TXRF technique (total reflection X-ray fluorescence) was employed to analyze the concentration of zinc (Zn) and other metals in the pineal gland of rats submitted to orally administered excess dose of Zn sulfate. The histochemical localization of Zn was also performed. TXRF results showed a 42.9% increase in Zn concentration, and alterations on the homeostasis of other essential elements in rats. It was concluded that TXRF is a suitable technique for measuring, for the first time in this work, the concentration of Zn accumulated in the pineal gland after administration of an excess dose of Zn, and this result may be either directly or indirectly related to alteration in the homeostasis of other chemical elements, such as S, Cl, K, Ca, Ti, Cr, Mn and Fe.

Pineal rat Zinc TXRF hyperzincemia

References (71)

  1. 1 Theory and Practice of Histological Techniques [DOI]
  2. 2 Rapid induction of Alzheimer A beta amyloid formation by zinc [DOI]
  3. 3 The Antioxidant Properties of Zinc [DOI]
  4. 4 The Role of Zinc in Selective Neuronal Death After Transient Global Cerebral Ischemia [DOI]
  5. 5 Zinc and its importance for human health: An integrative review.
  6. 6 Zinc transporters and the cellular trafficking of zinc [DOI]
  7. 7 Importance of Zinc in the Central Nervous System: The Zinc-Containing Neuron [DOI]
  8. 8 Metals and neuroscience [DOI]
  9. 9 ZnT-3, a putative transporter of zinc into synaptic vesicles [DOI]
  10. 10 Mammalian Zinc Transport, Trafficking, and Signals [DOI]
  11. 11 Zinc Homeostasis in Humans [DOI]
  12. 12 Zinc Metabolism in the Brain: Relevance to Human Neurodegenerative Disorders [DOI]
  13. 13 Total-reflection X-ray fluorescence analysis
  14. 14 A quinoline fluorescence method for visualizing and assaying the histochemically reactive zinc (bouton zinc) in the brain [DOI]
  15. 15 Effect of Heavy Metals on, and Handling by, the Kidney [DOI]
  16. 16 Metals and neurodegenerative diseases. A systematic review [DOI]
  17. 17 Manganese neurotoxicity: A focus on the neonate [DOI]
  18. 18 Oxidative damage and neurodegeneration in manganese-induced neurotoxicity [DOI]
  19. 19 Metallothionein: An overview [DOI]
  20. 20 Zinc Metabolism and Metallothioneins [DOI]
  21. 21 Responsive transporter genes within the murine intestinal-pancreatic axis form a basis of zinc homeostasis [DOI]
  22. 22 Evolution of photosensory pineal organs in new light: the fate of neuroendocrine photoreceptors [DOI]
  23. 23 Zinc neurotoxicity in cortical cell culture [DOI]
  24. 24 A simplified timm stain procedure compatible with formaldehyde fixation and routine paraffin embedding of rat brain [DOI]
  25. 25 Brief exposure to zinc is toxic to cortical neurons [DOI]
  26. 26 TRACE ELEMENT CONCENTRATION IN HUMAN BRAIN [DOI]
  27. 27 New Evidence of Iron and Zinc Interplay at the Enterocyte and Neural Tissues [DOI]
  28. 28 Uncertainties in the Environmental Risk Assessment of Metals [DOI]
  29. 29 Mechanisms by which metals promote events connected to neurodegenerative diseases [DOI]
  30. 30 Calcium and Phosphorus Homeostasis [DOI]
  31. 31 Metallothionein and Zinc Transporter Expression in Circulating Human Blood Cells as Biomarkers of Zinc Status: a Systematic Review [DOI]
  32. 32 Role of Zinc in Hemostasis: A Review [DOI]
  33. 33 X-Ray Fluorescence Microscopy Reveals Accumulation and Secretion of Discrete Intracellular Zinc Pools in the Lactating Mouse Mammary Gland [DOI]
  34. 34 The human pineal gland: Relationships with surrounding structures and blood supply [DOI]
  35. 35 Oral Zinc Supplementation May Improve Cognitive Function in Schoolchildren [DOI]
  36. 36 Zinc Supplementation or Regulation of its Homeostasis: Advantages and Threats [DOI]
  37. 37 Rat brain endothelial cells are a target of manganese toxicity [DOI]
  38. 38 The renaissance of mitochondrial calcium transport [DOI]
  39. 39 USE OF DITHIZONE AS AN EXTRACTANT TO ESTIMATE THE ZINC NUTRIENT STATUS OF SOILS [DOI]
  40. 40 Cytochemical techniques for zinc and heavy metals localization in nerve cells [DOI]
  41. 41 Neo-Timm staining in the thalamus of chronically epileptic rats [DOI]
  42. 42 Age-Related Changes in Tissue and Plasma Zinc Levels: Modulation by Exogenously Administered Melatonin [DOI]
  43. 43 Zinc and Copper Metabolism and Risk of Autism: a reply to Sayehmiri et al. [DOI]
  44. 44 Ultrastructural observations of pineal gland capillaries in four rodent species [DOI]
  45. 45 Trace element concentration in the human pineal body activation analysis of cobalt, iron, rubidium, selenium, zinc, antimony and cesium [DOI]
  46. 46 RECOGNITION OF N-ACETYLGLUCOSAMINE (GLyNAc) AND POLY-N-ACETYLLACTOSAMINE RESIDUES IN VESSELS OF THE RAT PINEAL GLAND [DOI]
  47. 47 Pineal Gland Post‐natal Growth in Rat Revisited [DOI]
  48. 48 Metallothionein-I–II expression in young and adult bovine pineal gland [DOI]
  49. 49 Clinical repercussions of zinc deficiency in human beings
  50. 50 The essential metals for humans: a brief overview [DOI]
  51. 51 The role of zinc, copper, manganese and iron in neurodegenerative diseases [DOI]
  52. 52 A Guide to Human Zinc Absorption: General Overview and Recent Advances of In Vitro Intestinal Models [DOI]
  53. 53 Zinc transporters and their functional integration in mammalian cells [DOI]
  54. 54 Iron and Zinc Homeostasis and Interactions: Does Enteric Zinc Excretion Cross-Talk with Intestinal Iron Absorption? [DOI]
  55. 55 Metals and Alzheimer’s Disease: How Far Have We Come in the Clinic? [DOI]
  56. 56 Zinc in the Brain: Friend or Foe? [DOI]
  57. 57 The Impact of Zinc and Zinc Homeostasis on the Intestinal Mucosal Barrier and Intestinal Diseases [DOI]
  58. 58 Zinc as a Neuromodulator in the Central Nervous System with a Focus on the Olfactory Bulb [DOI]
  59. 59 Analysis of Trace Elements in Human Brain: Its Aim, Methods, and Concentration Levels [DOI]
  60. 60 Role and Therapeutic Potential of Melatonin in Various Type of Cancers [DOI]
  61. 61 The interaction of zinc and the blood-brain barrier under physiological and ischemic conditions [DOI]
  62. 62 Manganese transport in mammals by zinc transporter family proteins, ZNT and ZIP [DOI]
  63. 63 Zinc in Dog Nutrition, Health and Disease: A Review [DOI]
  64. 64 Amyloids: Regulators of Metal Homeostasis in the Synapse [DOI]
  65. 65 Evolution and understanding of the d-block elements in the periodic table [DOI]
  66. 66 Zinc homeostasis and functions of zinc in the brain [DOI]
  67. 67 Water molecules mediate zinc mobility in the bacterial zinc diffusion channel ZIPB [DOI]
  68. 68 Investigation of the human pineal gland 3D organization by X-ray phase contrast tomography [DOI]
  69. 69 Review: Molecular structure and functions of zinc binding metallothionein-1 protein in mammalian body system.
  70. 70 Roles of Zinc Transporters That Control the Essentiality and Toxicity of Manganese and Cadmium [DOI]
  71. 71 Circumventricular Organs [DOI]

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