Angiogenesis and Antioxidant Activity of in vitro and in vivo Tuber of Ceropegia pusilla Wight and Arn
R. Prabakaran, K. Kalimuthu, C. Vani, C. Brindha
Journal of Pharmaceutical Research International · pp. 608–616 · Published 12 Jan 2014
10.9734/BJPR/2014/7481Abstract
Background: Angiogenesis refers to the formation of new blood vessels. This biological process lays an important role in embryonic development. Ceropegia pusilla (Asclepiadaceae) used in folk medicine system to treat various health problems. Present work deals with the analysis of antioxidant and antiangiogenic properties of in vivo and in vitro tuber extracts of C. pusilla by Chicken Egg Chorioallantoic Membrane Assay (CAM) in vivo. Methods: C. pusilla tubers of both in vivo and in vitro were extracted with ethanol and methanol. In vivo anti angiogenesis effect was studied by Chicken Egg Chorioallantoic Membrane (CAM) assay. The antioxidant properties were analysed using 1, -diphenyl-2-picryl- hydrazyl free radical scavenging assay and reducing power activity. Result: Ethanolic extract of both in vivo and in vitro tuber showed higher angiogenic activities 58.3±13.6 and 43.2±12.2 respectively than the methanolic extract. The average number of vessels in in vivo and in vitro ethanolic extracts treated CAM was 6.48 and 5.43. In vivo and in vitro extracts exhibits comparable DPPH radical scavenging activity and better reducing power activity. Conclusion: C. pusilla tuber extracts of both in vivo and in vitro inhibited angiogenesis by blocking normal vascularisation in chick embryo and also having better DPPH and reducing power activity.
References (16)
- 1 Tumor Angiogenesis: Therapeutic Implications [DOI]
- 2 Free Radicals in Biology and Medicine [DOI]
- 3 Angiogenesis in cancer, vascular, rheumatoid and other disease [DOI]
- 4 Role of vascular endothelial growth factor in regulation of physiological angiogenesis [DOI]
- 5 Determination and Involvement of Aqueous Reducing Compounds in Oxidative Defense Systems of Various Senescing Leaves [DOI]
- 6 Angiogenesis Assays: A Critical Overview [DOI]
- 7 Antioxidants: what role do they play in physical activity and health? [DOI]
- 8 Antioxidant properties of several commercial mushrooms [DOI]
- 9 Angiogenesis and apoptosis [DOI]
- 10 Inhibition of Cancer Cell Proliferation in Vitro by Fruit and Berry Extracts and Correlations with Antioxidant Levels [DOI]
- 11 Antioxidant activity of wines and relation with their polyphenolic composition [DOI]
- 12 Green tea catechins inhibit vascular endothelial growth factor receptor phosphorylation.
- 13 Angiogenesis and anti-angiogenesis activity of Chinese medicinal herbal extracts [DOI]
- 14 Delphinidin, a dietary anthocyanidin, inhibits vascular endothelial growth factor receptor-2 phosphorylation [DOI]
- 15 Ethnomedicinal plants used by Kani tribes of Agasthiyarmalai biosphere reserve, southern Western Ghats
- 16 Mechanism of angiogenesis
Cited by 13
C. Pradeepika, S. Nabi, N. Giri · 2018
K. Kalimuthu, V. Chinnadurai · 2014
C.G. Sangeetha, N. Jhansirani, Rashmi H.B. · Underutilized Tuber Crops of the Himalayan Region · 2026
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