Skip to content
Research Article Open access CC BY 4.0

Antibacterial Activity of Calycophyllum spruceanum Leaf Extract against Enterococcus faecalis Strains “In vitro” for Endodontic Purposes

Brena Barros Mendonça da Silva, Bruna Barros Mendonça da Silva, Giovana Pereira de Souza, Eder Ferreira de Arruda, Ana Bessa Muniz, Clarice Maia Carvalho, Rodrigo Asfury Rodrigues

Journal of Advances in Biology & Biotechnology · pp. 33–41 · Published 9 Mar 2023

10.9734/jabb/2023/v26i1615

Abstract

Aims: The indiscriminate use of antimicrobials contributed to the selection of multiresistant bacterial strains, mainly Enterococcus faecalis, leading to the search for new strategies for these infections, requiring the identification of new bioactive compounds from medicinal plants, an example is C. spruceanum used in traditional communities in the Amazon. In this sense, this study aimed to evaluate the antibacterial activity of C. spruceanum leaf extract against E. faecalis "in vitro" for endodontic purposes. Place and Duration of Study: Sample: the study was carried out at the microbiology laboratory of the Federal University of Acre (UFAC), in the city of Rio Branco, state of Acre, Brazil, from July 2021 to January 2022. Methodology: For this study, 50mg/mL of the crude extract of C. spruceanum leaf was diluted in Dimethylsulfoxide, as well as 50mg/mL of the extract with 1000mg of calcium hydroxide and 1000mg of calcium hydroxide in 1mL of distilled water. After preparing the extracts, 20µL were deposited in the wells made on Müeler-Hinton agar medium seeded with E. faecalis, incubated for 24 hours at 37°C. After this period, the positive activity of the extracts was obtained from the formation of the inhibition halo in millimeters. Results: The best results were for extract associated with calcium hydroxide (20mm), followed by calcium hydroxide solution (17.67mm) and with lower activity, C. spruceanum extract (14.33mm). The best results of the Minimum Inhibitory Concentration with values ​​of 6.25 mg/mL for the extract combined with calcium hydroxide and Minimum Bactericidal Concentration that was 25mg/mL for the association of the extract with calcium hydroxide. Conclusion: The C. spruceanum extract has antibacterial activity against E. faecalis and its association with calcium hydroxide potentiated its effect.

Enterococcus faecalis antibacterials amazon plant extracts bioactive compounds

References (15)

  1. 1 Intestinal colonization resistance [DOI]
  2. 2 Antibiotic Resistomes in Plant Microbiomes [DOI]
  3. 3 Endodontic infections: Concepts, paradigms, and perspectives [DOI]
  4. 4 Postoperative Pain in Multiple-visit and Single-visit Root Canal Treatment [DOI]
  5. 5 Characterization of Microbiota of Root Canal-Treated Teeth with Posttreatment Disease [DOI]
  6. 6 Antimicrobial Activity and Physicochemical Properties of Calcium Hydroxide Pastes Used as Intracanal Medication [DOI]
  7. 7 In vitro antimicrobial and cytotoxic effects of Anacardium occidentale and Mangifera indica in oral care [DOI]
  8. 8 Impact of an antibiotic restriction policy on hospital expenditures and bacterial susceptibilities: a lesson from a pediatric institution in a developing country [DOI]
  9. 9 An in Vitro Evaluation of Antimicrobial Efficacy of 2% Chlorhexidine Gel, Propolis and Calcium Hydroxide Against Enterococcus faecalis in Human Root Dentin [DOI]
  10. 10 Indole monoterpene alkaloids from Chimarrhis turbinata DC Prodr.: a contribution to the chemotaxonomic studies of the Rubiaceae family [DOI]
  11. 11 Antibacterial Efficacy of Triple Antibiotic Medication With Macrogol (3Mix-MP), Traditional Triple Antibiotic Paste, Calcium Hydroxide, and Ethanol Extract of Propolis: An Intratubular Dentin Ex Vivo Confocal Laser Scanning Microscopic Study [DOI]
  12. 12 Sesquiterpene Lactones from Cotula cinerea with Antibiotic Activity against Clinical Isolates of Enterococcus faecalis [DOI]
  13. 13 Biologically Active Extracts from Different Medicinal Plants Tested as Potential Additives against Bee Pathogens [DOI]
  14. 14 The Antibacterial Effect of Additional Photodynamic Therapy in Failed Endodontically Treated Teeth: A Pilot Study [DOI]
  15. 15 Portrayal of Punica granatum L. peel extract through High Performance Liquid Chromatography and antimicrobial activity evaluation [DOI]

Cited by 9

Phytochemical, Antioxidant and Antimicrobial Potentials of Azadirachta indica Leaf Extract Using Bacteria Isolated from Polluted Water as Test Organisms

Olusola Nathaniel Majolagbe, Bolatito Motunrayo Ishola, Ezekiel Gbadebo Adeyeni · Journal of Advances in Biology & Biotechnology · 2023

Showing 1 of 9 known citations — external sources report more than can currently be individually listed.

Related research

Article metrics

Real usage data collected on this platform.

29

Page views

0

PDF downloads

9

Outbound clicks

9

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.