Skip to content
Research Article Open access CC BY 4.0

Antibiotic Resistance Patterns of Lactic Acid Bacteria Isolated From Different Fermented Milk Products of Turkish Origin

Melihcan Ozteber, Gamze Başbülbül

Microbiology Research Journal International · pp. 1–13 · Published 25 Jun 2017

10.9734/MRJI/2017/33221

Abstract

Aims: Lactic acid bacteria isolated from 48 Turkish fermented milk products obtained from local markets, dairies or bazaars were investigated for their resistance of antibiotics including ampicillin, erythromycin, gentamicin, chloramphenicol, lincomycin, meropenem, ciprofloxacin, teicoplanin, tetracycline and vancomycin. Place and Duration of Study: Adnan Menderes University Biology Department, Microbiology laboratory, between 2012-2014 Methods: LAB strains, belonging to 14 species of Lactobacillus (n=68), 1 species of Lactococcus (n=16), 5 species of Enterococcus (n=14) and 2 species of Streptococcus (n=17) were isolated and identified at species level by their 16S rRNA gene sequencing. Minimal Inhibitory Concentrations (MIC) for 10 antibiotics were determined by agar dilution test using multipoint inoculator. Antibiotic resistance genes for erythromycin [erm(A), erm (B), erm (C)], gentamycin aac(6’) aph(2’’), chloramphenicol (cat), tetracycline [tet(K), tet(L), tet(M), tet(S), tet(Q)] and vancomycin [van(A), van(B), van(C), van(X)] were investigated in strains. Mating experiments were done with E. faecalis JH2-2 to detect the transferability of resistance genes. Results: Among 115 strains antibiotic resistance was detected against lincomycin (27,8%), tetracycline (20%), ampicillin (13,9%), meropenem (11,3%), gentamycin (10,4%), erythromycin (7,8%), ciprofloxacin (6,1%), chloramphenicol (3,4%), vancomycin (0,87%). While all these strains were susceptible to teicoplanin, 29,5% of isolates were multiple resistant to various antibiotics. The resistance genes aac(6’) aph(2’’), erm(B), tet(L), tet(M) and van(C) were detected in strains of,  Lb. delbrueckii subsp. bulgaricus, Lb. kefiri, Lc. lactis subsp. lactis, S. lutetiensis, S. macedonicus, E. faecalis, E. gallinarum isolated from some cheeses and one household kefir samples. Conclusion: Antibiotic-resistant LAB carrying transeferable resistance genes in some Turkish dairy products, may act as a dangerous vehicle for transmission of these traits to the other bacteria by horizontal gene transfer.

LAB antibiotic resistance fermented milk products resistance genes.

References (18)

  1. 1 Update on acquired tetracycline resistance genes [DOI]
  2. 2 Antibiotic resistance in food lactic acid bacteria—a review [DOI]
  3. 3 Molecular Ecology of Tetracycline Resistance: Development and Validation of Primers for Detection of Tetracycline Resistance Genes Encoding Ribosomal Protection Proteins [DOI]
  4. 4 Enterococci from foods [DOI]
  5. 5 Susceptibility of Lactobacillus spp. to antimicrobial agents [DOI]
  6. 6 Antimicrobial susceptibilities of Lactobacillus, Pediococcus and Lactococcus human isolates and cultures intended for probiotic or nutritional use [DOI]
  7. 7 Characterization and Transfer of Antibiotic Resistance in Lactic Acid Bacteria from Fermented Food Products [DOI]
  8. 8 Evaluation of New Broth Media for Microdilution Antibiotic Susceptibility Testing of Lactobacilli, Pediococci, Lactococci, and Bifidobacteria [DOI]
  9. 9 Prevalence and Molecular Characterization of Tetracycline Resistance in Enterococcus Isolates from Food [DOI]
  10. 10 Antibiotic resistance of lactic acid bacteria and Bifidobacterium spp. isolated from dairy and pharmaceutical products [DOI]
  11. 11 Resistance of potential probiotic lactic acid bacteria and bifidobacteria of African and European origin to antimicrobials: Determination and transferability of the resistance genes to other bacteria [DOI]
  12. 12 Assessment of antibiotic susceptibility within lactic acid bacteria strains isolated from wine [DOI]
  13. 13 Antimicrobial susceptibility of starter culture bacteria used in Norwegian dairy products [DOI]
  14. 14 Vancomycin-resistant enterococci isolated from animals and food [DOI]
  15. 15 Identification of lactic acid bacteria isolated from traditional drinking yoghurt in tribes of Fars province [DOI]
  16. 16 Characterization of Streptococcus macedonicus strains isolated from artisanal Italian raw milk cheeses [DOI]
  17. 17 Antibiotic resistance profile of lactic acid bacteria and their implications in food chain.
  18. 18 Role of milk and meat products as vehicles for antibiotic-resistant bacteria.

Cited by 13

Antibiotic resistance of Lactobacillus fermentum isolates of dadiah Lintau

R. Amelia, H. Harun, Y. E. Pratama · IOP Conference Series: Earth and Environment · 2024

Characterization and technological functions of different lactic acid bacteria from traditionally produced Kırklareli white brined cheese during the ripening period

Bayram Çetin, Merve Usal, H. Aloğlu · Folia Microbiologica (Prague) · 2024

Technological and probiotic potential of autochthonous lactic acid bacteria from spontaneously fermented dromedary milk

Olfa Oussaief, Zeineb Jrad, Imed Sbissi · Journal of food processing and preservation · 2020

Genomic and phenotypic assessments of safety and probiotic properties of Streptococcus macedonicus strains of dairy origin.

A. Tarrah, V. da Silva Duarte, S. Pakroo · Food Research International · 2020

Differences in Carbohydrates Utilization and Antibiotic Resistance Between Streptococcus macedonicus and Streptococcus thermophilus Strains Isolated from Dairy Products in Italy

A. Tarrah, L. Treu, Sabrina Giaretta · Current Microbiology · 2018

Potential Probiotic Activity of Lactic Acid Bacteria Isolated from Vended Dairy Products

Mohamed Abdel baser, Samy M. Abdelhamid, A. Radwan · Middle East Journal of Applied Sciences · 2021

The sensitivity profile of lactic acid bacterial isolate antibiotics with biopreservative potential

Charis Amarantini, Vinsa Cantya Prakasita, Chrismelan Julia Pesoa · AIP Conference Proceedings · 2023

Showing 7 of 13 known citations — external sources report more than can currently be individually listed.

Related research

Article metrics

Real usage data collected on this platform.

39

Page views

0

PDF downloads

4

Outbound clicks

13

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.