Antimicrobial Activities of the Endophytic Fungus, Fusarium equiseti, Isolated from The leaves of Ocimum gratissimum
Nnamdi Markben Adione, Ifeanyi Peter Onyeka, Chika Christiana. Abba, Nkeoma Nkasi Okoye, Peter Maduabuchi Eze, Blessing Ogechukwu. Umeokoli, OgechI Ozioma. Anyanwu, Festus Basden Chiedu Okoye
Journal of Advances in Medical and Pharmaceutical Sciences · pp. 11–23 · Published 1 Aug 2022
10.9734/jamps/2022/v24i730312Abstract
Introduction: Fungal endophytes of medicinal plants origin are gaining increasing interest as sources of novel bioactive agents with therapeutic capacity. This study was designed to identify and evaluate secondary metabolites isolated from Fusarium equiseti; an endophytic fungus isolated from leaves of Ocimum gratissimum for their antimicrobial potentials. Methods: The isolation and molecular identification of the endophytic fungus, as well as fermentation, and extraction of secondary metabolites were carried out following standard laboratory procedures. The crude extract was partially purified by partitioning into different fractions using column chromatographic techniques and the fractions were tested for antimicrobial activity. The phytoconstituents in the bioactive fractions were detected by dereplication using High-Performance Liquid Chromatography linked with Diode Array Detection (HPLC-DAD). Results: Our findings showed that fraction 4 (DCM/ Methanol fraction 95:5) exhibited moderate to strong inhibition against the test micro-organisms namely, Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Candida albicans with MIC values in the range of 0.03 to 1 mg/mL but had no inhibition against Aspergillus niger and Salmonella typhi. The compounds detected in the HPLC assay include Phomoxanthone A, Scalarolide, equisetin, epi-equisetin an episomer of equisetin, Naamine A, Carbonarone A, destruxin B, cytosporin G, and WLIP (β-hydroxydecanoyl-Leu1-D Glu-De-Thr-Dval-DLeu3 DSer-Leu-Dser-Ile). These compounds have been reported to possess antimicrobial activity. Conclusion: The detection of these metabolites further confirms the potential of the endophytic fungus, Fusarium equiseti isolated from leaves of Ocimum gratissimum as a potential source of bioactive molecules for treatment of infectious diseases.
References (23)
- 1 Natural Products from Endophytic Microorganisms [DOI]
- 2 Masked mycotoxins: A review [DOI]
- 3 Reactive Oxygen Species Play a Role in Regulating a Fungus–Perennial Ryegrass Mutualistic Interaction [DOI]
- 4 Genetic diversity of Ocimum gratissimum L. based on volatile oil constituents, flavonoids and RAPD markers [DOI]
- 5 Balancing the generation and elimination of reactive oxygen species [DOI]
- 6 Antimicrobial activity of leaf extracts of Ocimum gratissimum on selected diarrhoea causing bacteria in southwestern Nigeria [DOI]
- 7 Production of Destruxins from Metarhizium spp. Fungi in Artificial Medium and in Endophytically Colonized Cowpea Plants [DOI]
- 8 Antimicrobial Lipids from Plants and Marine Organisms: An Overview of the Current State-of-the-Art and Future Prospects [DOI]
- 9 Antifungal activity from Ocimum gratissimum L. towards Cryptococcus neoformans [DOI]
- 10 Isolation and Characterization of Novel Human Immunodeficiency Virus Integrase Inhibitors from Fungal Metabolites [DOI]
- 11 Naamines and naamidines, novel imidazole alkaloids from the calcareous sponge leucetta chagosensis [DOI]
- 12 Eugenol and Methyl Eugenol Chemotypes of Essential Oil of Species Ocimum gratissimum L. and Ocimum campechianum Mill. from Colombia [DOI]
- 13 Anti-Cryptococcal and Nitric Oxide Synthase Inhibitory Imidazole Alkaloids from the Calcareous Sponge Leucetta cf chagosensis [DOI]
- 14 Metabolites from Combretum dolichopetalum and its associated endophytic fungus Nigrospora oryzae — Evidence for a metabolic partnership [DOI]
- 15 Antimicrobial activities of secondary metabolites of endophytic fungi isolated from Catharanthus roseus [DOI]
- 16 Antibacterial activity of Ocimum gratissimum (scent leaf) on some pathogenic gastrointestinal bacteria [DOI]
- 17 Suppressive effects of destruxin B on hepatitis B virus surface antigen gene expression in human hepatoma cells [DOI]
- 18 Antimicrobial Activity of Metabolites of an Endophytic Fungus Isolated from the Leaves of Citrus jambhiri (Rutaceae) [DOI]
- 19 Three New Scalarane Sesterterpenoids from the Okinawan Sponge Hyrtios erectus [DOI]
- 20 Cytosporins F–K, new epoxyquinols from the endophytic fungus Pestalotiopsis theae [DOI]
- 21 Ocimum gratissimum: A Review on Ethnomedicinal Properties, Phytochemical Constituents, and Pharmacological Profile [DOI]
- 22 Proceedings of the National Academy of Sciences [DOI]
- 23 African Journal of Microbiology Research [DOI]
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