Skip to content
Research Article Open access CC BY 4.0

Antimicrobial Resistance in Pasteurella multocida Type B and Mannheimia haemolytica Isolates in Cattle and Buffaloes

M. A. R. Priyantha, G. I. S. Perera, Nayani Medagama, D. M. S. B. Dissanayake, P. S. De Alwis, M. I. Wijemuni, N. G. N. Samarakoon, P. S. Fernando

Asian Journal of Research in Animal and Veterinary Sciences · pp. 263–269 · Published 25 May 2021

10.9734/ajravs/2021/v4i3139

Abstract

Hemorrhagic septicemia is a fatal septicemic disease in cattle and buffaloes in tropical countries. Young animals were more susceptible, and buffaloes were shown high percentage of mortality than cattle. Mannheimia haemolytica was described as an opportunistic pathogen in cattle and buffaloes and concurrent infection were also reported with primary other diseases. Only few studies were published in the country on these two bacterial organisms, information on antimicrobials resistance was limited in local herds. Lack of clinical breakpoints or host specific clinical breakpoints has been identified as a limiting factor to identify the phenotypic resistance. The objective of the study was to confirm the Pasteurella multocida isolates which received from clinical cases of hemorrhagic septicemia in the country. The second objectives were to determine phenotypic antimicrobial resistance in Pasteurella multocida clinical isolates and to compare with phenotypic antimicrobial; resistant phenotypes of Mannheimia haemolytica. The clinical isolates of Pasteurella multocida were received (n=29) from the regional laboratories in the country from 2017-2019. Manhenimia hemolytica (n=49) was collected from our laboratory collection which were sampled from cattle and buffaloes from dry zone of the country previously. The resistance percentage of were Pasteurella multocida 72% and 29% for ciprofloxacin and cefotaxime. Resistance of cefotaxime was shown in Pasteurella multocida and none in Manhenimia hemolytica. Resistances to ciprofloxacin were shown high in Pasteurella multocida and low in Manhenimia hemolytica. Resistance to amoxicillin and clavulanic acid and tetracycline were found in both organisms. Phenotypic antimicrobial resistance was reported low in Pasteurella multocida and Manhenimia hemolytica in cattle and buffaloes. In conclusion, usage of sulfamethoxazole and quinolone group of antimicrobials for the pasteurellosis such as enrofloxacin, ciprofloxacin may have negative impact due to the high frequency of resistance observed.

Pathogen bacterial organisms buffaloes antimicrobials Antimicrobial resistance

References (18)

  1. 1 Antimicrobial agents and chemotherapy—1969 [DOI]
  2. 2 THE JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY [DOI]
  3. 3 Structured Literature Review of Responses of Cattle to Viral and Bacterial Pathogens Causing Bovine Respiratory Disease Complex [DOI]
  4. 4 Prevalence and antimicrobial susceptibility of Mannheimia haemolytica, Pasteurella multocida, and Histophilus somni isolated from the lower respiratory tract of healthy feedlot cattle and those diagnosed with bovine respiratory disease [DOI]
  5. 5 Molecular and immunological characterization of Pasteurella multocida serotype A:3 OmpA: evidence of its role in P. multocida interaction with extracellular matrix molecules [DOI]
  6. 6 Virulence genes and antimicrobial susceptibility in Pasteurella multocida isolates from calves [DOI]
  7. 7 Plasmid-borne florfenicol resistance in Pasteurella multocida [DOI]
  8. 8 Occurrence of virulence factors and antimicrobial resistance in Pasteurella multocida strains isolated from slaughter cattle in Iran [DOI]
  9. 9 Comparative Genomic Analysis of Asian Haemorrhagic Septicaemia-Associated Strains of Pasteurella multocida Identifies More than 90 Haemorrhagic Septicaemia-Specific Genes [DOI]
  10. 10 Antimicrobial resistance of Pasteurella multocida type B isolates associated with acute septicemia in pigs and cattle in Spain [DOI]
  11. 11 Serotyping, Genotyping and Virulence Genes Characterization of Pasteurella multocida and Mannheimia haemolytica Isolates Recovered from Pneumonic Cattle Calves in North Upper Egypt [DOI]
  12. 12 Genetic and phenotypic characterization of tetracycline-resistant Pasteurella multocida isolated from pigs [DOI]
  13. 13 In-vitro phagocytosis and intracellular killing of Pasteurella multocida B:2 by phagocytic cells of buffaloes [DOI]
  14. 14 Antimicrobial resistance among Pasteurella spp recovered from Missouri and Iowa cattle with bovine respiratory disease complex.
  15. 15 Characterisation of a multiresistant Pasteurella multocida strain isolated from cattle [DOI]
  16. 16 Antimicrobial resistance evaluation of Iranian ovine and bovine Pasteurella multocida.
  17. 17 Rapid detection of tetracycline resistance in bovine Pasteurella multocida isolates by MALDI Biotyper antibiotic susceptibility test rapid assay (MBT-ASTRA)
  18. 18 haemorrhagic septicaemia [DOI]

Cited by 0

No indexed citations yet.

Related research

Article metrics

Real usage data collected on this platform.

50

Page views

0

PDF downloads

4

Outbound clicks

0

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.