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Research Article Open access CC BY 4.0

AntimicrobialResistance and Virulence of Shiga-Toxin-Producing Escherichia coli from Milk Samples of Some Cattle Farms Al-Buḥayrah Governorate Egypt

Mohamed Sabry Abd Elraheam Elsayed, Alaa Eldin Hussein, Samaa Mahmoud Mohamed Abutabeikh

Journal of Advances in Microbiology · pp. 20–30 · Published 17 Feb 2024

10.9734/jamb/2024/v24i2792

Abstract

Objective: Contamination of milk with Escherichia coli (E. coli) can pose significant public health and economic concerns. The current study was conducted to explore the prevalence and antibiotic resistance profiles of Shiga-toxin-producing Escherichia coli in milk samples of dairy cows in Egypt. Study Design: Twenty milk samples were gathered from dairy cattle (ten from healthy cows and ten from mastitic cows) and examined for the presence of Shiga-toxin-producing E. coli using Eosin Methylene Blue agar. Molecular detection of virulence genes and phenotypic antimicrobial resistance were performed. Results: E. coli was isolated from 65% (13/20) of the total milk samples (90% from mastitic cows and 40% from normal healthy cows). The virulence gene profiling of E.coli by polymerase chain reaction revealed that 84.62% (11/13) were Stx2a, 69.23% (9/13) were Stx2f, and 61.54% (8/13) were positive for Stx1 and Stx2 similarly. All the isolates showed resistance to Amikacin, Ciprofloxacin, Erythromycin, Linezolid, Penicillin G, and Trimethoprim/Sulfamethoxazole, while 92.3% expressed resistance to Amoxicillin/ Clavulanic acid, and Tylosin, and 84.62% of the isolates were resistant to Oxytetracycline indicating multidrug-resistant profiles. Conclusion: The present work highlights the presence of various E. coli strains, including potential pathogens and multidrug-resistant isolates, in milk samples from dairy cows. Regular milk testing and improved mastitis control measures are the key to reduce the public health risks and to safeguarde dairy farm productivity.

E. coli milk dairy cows virulence genes antibiotic resistance

References (47)

  1. 1 Pathogenesis and Diagnosis of Shiga Toxin-Producing Escherichia coli Infections [DOI]
  2. 2 The complex microbiota of raw milk [DOI]
  3. 3 Foodborne Pathogens in Milk and the Dairy Farm Environment: Food Safety and Public Health Implications [DOI]
  4. 4 Enterohaemorrhagic Escherichia coli in human medicine [DOI]
  5. 5 Impact of Antibiotic Use in Adult Dairy Cows on Antimicrobial Resistance of Veterinary and Human Pathogens: A Comprehensive Review [DOI]
  6. 6 Trends in Antibacterial Susceptibility of Mastitis Pathogens During a Seven-Year Period [DOI]
  7. 7 Unpasteurized Milk: A Continued Public Health Threat [DOI]
  8. 8 ANTIMICROBIAL DRUG RESISTANCE IN STRAINS OF Escherichia coli ISOLATED FROM FOOD SOURCES [DOI]
  9. 9 Antimicrobial resistance of Escherichia coli and therapeutic implications [DOI]
  10. 10 Prevalence and pathogenic potential of Escherichia coli isolates from raw milk and raw milk cheese in Egypt [DOI]
  11. 11 Prevalence, identification of virulence factors, O-serogroups and antibiotic resistance properties of Shiga-toxin producing Escherichia coli strains isolated from raw milk and traditional dairy products [DOI]
  12. 12 High prevalence of antibiotic resistance in pathogenic foodborne bacteria isolated from bovine milk [DOI]
  13. 13 Prevalence and antimicrobial-resistance phenotypes and genotypes of Escherichia coli isolated from raw milk samples from mastitis cases in four regions of China [DOI]
  14. 14 Prevalence of Antimicrobial Resistance and Characteristics of Escherichia coli Isolates From Fecal and Manure Pit Samples on Dairy Farms in the Province of Québec, Canada [DOI]
  15. 15 Prevalence and mechanisms of antibiotic resistance in Escherichia coli isolated from mastitic dairy cattle in Canada [DOI]
  16. 16 Occurrence and Characteristics of Extended-Spectrum β-Lactamase-Producing Escherichia coli from Dairy Cattle, Milk, and Farm Environments in Peninsular Malaysia [DOI]
  17. 17 Shiga toxin-producing Escherichia coli strains from bovines: association of adhesion with carriage of eae and other genes [DOI]
  18. 18 Occurrence of Clinical and Sub-Clinical Mastitis in Dairy Herds in the West Littoral Region in Uruguay [DOI]
  19. 19 Escherichia coli Isolates’ Serotypes, Genotypes, and Virulence Genes and Clinical Coliform Mastitis Severity [DOI]
  20. 20 Occurrence, virulence genes and antibiotic resistance of Escherichia coli O157 isolated from raw bovine, caprine and ovine milk in Greece [DOI]
  21. 21 Prevalence, bacterial causes, and antimicrobial susceptibility profile of mastitis isolates from cows in large-scale dairy farms of Northern Ethiopia [DOI]
  22. 22 Phylogeny, virulence factors and antimicrobial susceptibility of Escherichia coli isolated in clinical bovine mastitis [DOI]
  23. 23 Escherichia coli from clinical mastitis [DOI]
  24. 24 Concise Review of Veterinary Microbiology
  25. 25 Detection and drug resistance profile of Escherichia coli from subclinical mastitis cows and water supply in dairy farms in Saraburi Province, Thailand [DOI]
  26. 26 Automated electronic medical record sepsis detection in the emergency department [DOI]
  27. 27 Shiga Toxin-ProducingEscherichia coliIsolated from Bovine Mastitic Milk: Serogroups, Virulence Factors, and Antibiotic Resistance Properties [DOI]
  28. 28 Major causes of mastitis and associated risk factors in smallholder dairy farms in and around Hawassa, Southern Ethiopia [DOI]
  29. 29 Prevalence of bovine subclinical mastitis, its etiology and diagnosis of antibiotic resistance of dairy farms in four municipalities of a tropical region of Mexico [DOI]
  30. 30 Development of a serogroup-specific multiplex PCR assay to detect a set of Escherichia coli serogroups based on the identification of their O-antigen gene clusters [DOI]
  31. 31 Current status of cattle production system in Nyagatare District-Rwanda [DOI]
  32. 32 Shiga Toxin–Producing Escherichia coli in Mastitis: An International Perspective [DOI]
  33. 33 Evaluation of virulence factors profiles and antimicrobials resistance of Escherichia coli isolated from bulk tank milk and raw milk filters [DOI]
  34. 34 Molecular Characterization of Multidrug-Resistant Escherichia coli Isolated from Milk of Dairy Cows with Clinical Mastitis in Algeria [DOI]
  35. 35 The Distribution ofEscherichia coliSerovars, Virulence Genes, Gene Association and Combinations and Virulence Genes Encoding Serotypes in PathogenicE. coliRecovered from Diarrhoeic Calves, Sheep and Goat [DOI]
  36. 36 Virulence Factors of Escherichia coli Isolated from Recurrent Cases of Clinical and Subclinical Mastitis in Buffaloes
  37. 37 Serotypes, Virulence Genes, and Intimin Types of Shiga Toxin-Producing Escherichia coli and Enteropathogenic Escherichia coli Isolated from Mastitic Milk Relevant to Human Health in Egypt [DOI]
  38. 38 Molecular Detection and Antimicrobial Resistance Patterns of Shiga Toxigenic Escherichia coli Isolated from Bovine Subclinical Mastitis Milk Samples in Kurdistan, Iran. [DOI]
  39. 39 Genetic and antimicrobial resistance profiles of non-O157 Shiga toxin-producing Escherichia coli from different sources in Egypt [DOI]
  40. 40 Current Status of Antimicrobial Resistance and Prospect for New Vaccines against Major Bacterial Bovine Mastitis Pathogens [DOI]
  41. 41 A contribution on Coliforms causing mastitis in cows with reference to serotypes and virulence factors of E. coli isolates [DOI]
  42. 42 Virulence Properties of Escherichia coli Isolated from Clinical Bovine Mastitis
  43. 43 Bovine Mastitis: Prevalence, Isolation of Bacterial Species Involved and its Antimicrobial Susceptibility Test around Debrezeit, Ethiopia [DOI]
  44. 44 Genome-Based Characterization of Multidrug-Resistant Escherichia coli Isolated from Clinical Bovine Mastitis [DOI]
  45. 45 Prevalence, Drug Resistance, and Virulence Genes of Potential Pathogenic Bacteria in Pasteurized Milk of Chinese Fresh Milk Bar [DOI]
  46. 46 Short Communication ‐ Prevalence of Verotoxin‐Producing Escherichia coli (VTEC) in Bovine Coli Mastitis and their Antibiotic Resistance Patterns [DOI]
  47. 47 Molecular Studies on E.coli Isolate from Milk of Mastitic Cattle with Special Reference to Associated Biochemical Changes in Kaliobea Governorate [DOI]

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