Application of SSR Markers for Genetic Purity Analysis of Parental Inbred Lines and Some Commercial Hybrid Maize (Zea mays L.)
I. O. Daniel, J. A. Adetumbi, O. O. Oyelakin, S. A. Olakojo, M. O. Ajala, S. O. Onagbesan
Journal of Experimental Agriculture International · pp. 597–606 · Published 28 Aug 2012
10.9734/AJEA/2012/1579Abstract
Aims: Morphological evaluation of seeds and growing plants used for certification for purity and variety distinctness in Nigeria is time consuming and expensive. This experiment set to evaluate the usefulness of SSR markers to determine genetic purity of commercial hybrids and their inbred lines. Place and Duration of Study: Bioscience unit, International Institute of Tropical Agriculture, Nigeria in December, 2011 Methodology: Seedlings of four F1 hybrids and four inbred lines were grown in the screen house of IITA for DNA extraction using Dellaporta method with some modifications. Six Simple Sequence Repeat (SSR) markers were used for Polymerase Chain Reaction (PCR) using Touch-Down PCR profile. The analysis is by fragment analysis as present (1) or absent (0) Mathematical equation to determine genetic purity of the genotypes was developed from the genetic distances matrix. Results: Simple descriptive analysis revealed that average genetic diversity and polymorphism information content (PIC) recorded by the markers was 0.592 and 0.512 respectively. Genetic purity level of inbred lines ranged between 91.3% and 98.7% while the hybrids ranged between 81.3% and 95%. Conclusion: SSR markers are powerful biotechnological tool capable of detecting genetic purity status of Nigerian maize hybrids therefore inclusion of DNA analysis of seeds using SSR markers to determine genetic purity of maize seed is recommended. However, further research work with larger number of seed samples per variety will be needed to validate reliability.
References (13)
- 1 A plant DNA minipreparation: Version II [DOI]
- 2 PowerMarker: an integrated analysis environment for genetic marker analysis [DOI]
- 3 Maize research and production in Nigeria [DOI]
- 4 Assessment of purity of rice hybrids using microsatellite and STS markers [DOI]
- 5 Identification of Canadian durum wheat varieties using a single PCR [DOI]
- 6 Tassel Morphology as an Indicator of Potential Pollen Production in Maize [DOI]
- 7 Hybrid authentication in upland cotton through RAPD analysis
- 8 Molecular Characterization of Black Bengal and Jamuna Pari Goat Breeds By RAPD Markers [DOI]
- 9 Identification of candidate gene–based SSR markers for lysine and tryptophan metabolic pathways in maize (Zea mays) [DOI]
- 10 Genetic purity certificate in seeds of hybrid maize using molecular markers [DOI]
- 11 An improved method of extracting genomic DNA from preserved tissues of Capsicum annuum for PCR amplification
- 12 Genetic diversity of maize inbred lines revealed by AFLP markers.
- 13 Use of SSR data to determine relationships among early maturing Iranian maize inbred lines
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