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Research Article Open access CC BY 4.0

Assessment of Acetylcholinesterase Enzyme (AChE) Activity in the Developing Brain of TSD Lizard, Calotes Versicolor (Daud.)

Rahul M. Handi, Laxmi S. Inamdar (Doddamani)

Asian Journal of Research in Zoology · pp. 58–64 · Published 10 Feb 2024

10.9734/ajriz/2024/v7i1140

Abstract

Acetylcholinesterase (AChE) is a critical enzyme in the neuronal cholinergic system of vertebrates. It is one of the efficient cholinesterases that is involved in the termination of acetylcholine-mediated neurotransmission, rapidly hydrolyzing acetylcholine into acetate and choline. Barring a few reports on the distribution/presence of AChE enzyme activity in the adult reptilian species there are nil reports on the presence of this enzyme activity in the developing brain. Hence, the present investigation aims to evaluate AChE enzyme activity in the developing brain of an oviparous lizard, Calotes versicolor which exhibits a novel Female-Male-Female-Male (FMFM) pattern of temperature-dependent sex determination (TSD). The eggs were collected during breeding season, padded with moist cotton, and incubated in an incubator at 30o C. The whole brain from the embryos was collected during different developmental stages from oviposition (Stage 27) to hatching (stage 42). The AChE enzyme activity was quantified in the brain, according to Ellman’s protocol using a spectrophotometer. The findings reveal that the onset of AChE enzyme activity is observed as early as at oviposition, which reflects the early action of AChE activity in the developing brain. The observed results albeit indirectly suggest the involvement of AChE enzyme activity in the morphogenetic process. Further, an exponential increase in AChE activity during the post-gonadal differentiation phase indicates age-related elevation in AChE enzyme activity which in turn unveils its involvement in neuronal transmission of the embryonic brain. Besides, this specific AChE enzyme activity in the developing brain of Calotes versicolor represents a conserved pattern for the cholinergic system in vertebrates.

Acetylcholinesterase enzyme oviparous lizard embryonic brain

References (19)

  1. 1 A new and rapid colorimetric determination of acetylcholinesterase activity [DOI]
  2. 2 The cholinergic system in the pathophysiology and treatment of Alzheimer’s disease [DOI]
  3. 3 Acetylcholinesterase — new roles for an old actor [DOI]
  4. 4 THE ACTION OF CERTAIN ESTERS AND ETHERS OF CHOLINE, AND THEIR RELATION TO MUSCARINE [DOI]
  5. 5 Acetylcholinesterase as a Biomarker in Environmental and Occupational Medicine: New Insights and Future Perspectives [DOI]
  6. 6 Modified Ellman procedure for assay of cholinesterases in crude enzymatic preparations [DOI]
  7. 7 Cholinesterase in embryonic development.
  8. 8 Axonogenesis and morphogenesis in the embryonic zebrafish brain [DOI]
  9. 9 Analysis of the development of the lizard, Calotes versicolor. I. A series of normal stages in the embryonic development [DOI]
  10. 10 A tropical oviparous lizard, Calotes versicolor, exhibiting a potentially novel FMFM pattern of temperature‐dependent sex determination [DOI]
  11. 11 Cholinesterases during development of the avian nervous system [DOI]
  12. 12 Acetylcholinesterase in prenatal rat heart: A marker for the early development of the cardiac conductive tissue? [DOI]
  13. 13 Distribution of acetylcholinesterase activity in the rat embryonic heart with reference to HNK-1 immunoreactivity in the conduction tissue [DOI]
  14. 14 Developmental expression of acetyl- and butyrylcholinesterase in the rat: enzyme and mRNA levels in embryonic dorsal root ganglia [DOI]
  15. 15 Expression of cholinergic system molecules during development of the chick nervous system [DOI]
  16. 16 Development of the Adrenal Gland in the Tropical Lizard Calotes versicolor [DOI]
  17. 17 Topographical distribution of acetylcholinesterase and butyrylcholinesterase in the diencephalon and mesencephalon of the garden lizard (Calotes versicolor) [DOI]
  18. 18 Early appearance of acetylcholinergic, serotoninergic, and peptidergic neurons and fibers in the developing human central nervous system [DOI]
  19. 19 Topographical distribution of acetylcholinesterase and butyrylcholinesterase in the cerebral hemisphere of the garden lizard, calotes versicolor [DOI]

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