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Research Article Open access CC BY 4.0

Detection of Extended Spectrum Beta-Lactamase Production in Escherichia coli Isolated from Cattle Faeces in Owo Metropolis

O. A. Adeluwoye-Ajayi, F. M. Thomas, R. R. Awoniyi

International Journal of Pathogen Research · pp. 32–39 · Published 22 Dec 2021

10.9734/ijpr/2021/v8i430212

Abstract

Escherichia coli which synthesize extended spectrum beta-lactamases (ESBL) have been implicated in severe human diseases. There is substantial evidence that cattle faeces have a role in developing and spread of multidrug-resistant pathogens, raising public health concerns. The study is aimed at detecting ESBL-producing E. coli associated with cattle faeces within Owo metropolis. Freshly passed faecal samples were taken aseptically from 9 apparently healthy cattle that were about to be slaughtered and placed in correctly labeled sterile capped universal bottles with sterile spatula. Using standardized method, Escherichia coli was isolated on Eosin Methylene Blue Agar. The antibiotic susceptibility of the isolates was evaluated using the Kirby Bauer disc diffusion method, and the expression of ESBL was detected by the double disc synergy test. According to the findings, 25 (55.6%) of the 45 E. coli isolates showed probable production of ESBL during screening and 11 (44.0%) were confirmed to be ESBL producers. The isolates' antibiotic resistance pattern revealed that they were most resistant to gentamicin (81.8%) and least resistant to imipenem (9.1%). As a result, imipenem was the most effective antibiotic against ESBL-producing E. coli isolates. Antibiotypes of ESBL-producing E. coli isolates also showed that 9 (81.8%) of ESBL-positive E. coli isolates were multidrug-resistant. This studies confirmed that E. coli isolated from bovine faeces had high prevalence of antibiotic resistance and revealed that ESBL-producing bacteria are present in cattle and subsequent consumers. This necessitates the implementation of mitigating strategies to limit the transfer of antibiotic-resistant bacteria from animals to humans.

ESBL E. coli antibiotic resistance cattle faeces

References (18)

  1. 1 Understanding the mechanisms and drivers of antimicrobial resistance [DOI]
  2. 2 THE JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY [DOI]
  3. 3 Medical Consequences of Antibiotic Use in Agriculture [DOI]
  4. 4 Antibiotic resistance is the quintessential One Health issue [DOI]
  5. 5 Antibiotic resistance and extended spectrum beta-lactamases: Types, epidemiology and treatment [DOI]
  6. 6 Antimicrobial resistance—a threat to the world’s sustainable development [DOI]
  7. 7 Detection of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in vegetables, soil and water of the farm environment in Tunisia [DOI]
  8. 8 High levels of multidrug resistance in clinical isolates of Gram-negative pathogens from Nigeria [DOI]
  9. 9 Occurrence and characteristics of extended spectrum beta-lactamases-producing Enterobacteriaceae from foods of animal origin [DOI]
  10. 10 Non-ST131 Escherichia coli from cattle harbouring human-like blaCTX-M-15-carrying plasmids [DOI]
  11. 11 ESBL-Producing Enterobacteriaceae: Occurrence, Risk Factors for Fecal Carriage and Strain Traits in the Swiss Slaughter Cattle Population Younger than 2 Years Sampled at Abattoir Level [DOI]
  12. 12 Occurrence of extended-spectrum beta-lactamases in human and bovine isolates of Escherichia coli from Oyo state, Nigeria [DOI]
  13. 13 A One Health Comparative Assessment of Antimicrobial Resistance in Generic and Extended-Spectrum Cephalosporin-Resistant Escherichia coli from Beef Production, Sewage and Clinical Settings [DOI]
  14. 14 Multidrug resistant Escherichia coli isolates of poultry origin in Abeokuta, South Western Nigeria
  15. 15 Molecular Characterization of Escherichia coli Strains That Cause Symptomatic and Asymptomatic Urinary Tract Infections [DOI]
  16. 16 Antibiotic Resistance among Commensal Escherichia coli Isolated from Faeces of Cattle in Ado-Ekiti, Nigeria [DOI]
  17. 17 The Antibiotic Resistance Problem Revisited [DOI]
  18. 18 Applied and Environmental Microbiology [DOI]

Cited by 5

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Akerele, Y. E., Onuoha, C. C., Udofia, E. V. · UMYU Journal of Microbiology Research (UJMR) · 2024

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