Development and Validation of Novel RP-HPLC Method for the Simultaneous Determination of Remogliflozin and Vildagliptin in Bulk and in synthetic Mixture
Drashti A. Mandale, Chainesh Shah, Rakesh Jatt
Journal of Pharmaceutical Research International · pp. 338–349 · Published 14 Aug 2021
10.9734/jpri/2021/v33i40B32296Abstract
Vildagliptin which is DPP-4 inhibitor and Remogliflozin which is SGLT2 inhibitor in single dose regimen lower blood glucose by separate, complementary mechanisms. Both are glucose dependent, accounting for the low risk of hypoglycaemia during treatment. There is no risk factors associated with this combination and moreover it is single dose regimen. The aim of the present study was to develop and validate a simple, rapid and reproducible gradient high performance reverse phase liquid chromatography method for the estimation of Remogliflozin and Vildagliptin in bulk drug sample and in synthetic mixture using Xterra® Waters C18 column (150 mm×4.6 mm, 5 µm) at 25°C with UV detection at 210 nm and for this gradient mode was used. The compounds were eluted gradiently at a flow rate of 1.0ml/min. The average retention times for Remogliflozin and Vildagliptin were 4.881 and 6.334 min, respectively. The calibration curves were linear (r2 =0.988) over the concentration range 10-200 µg/ml for Remogliflozin and 10-200 µg/ml for Vildagliptin. No spectral or chromatographic interferences from formulation excipients were found and hence it was successfully applied for the determination of Remogliflozin and Vildagliptin in bulk and in synthetic mixture. The accuracy of the proposed method was determined by recovery studies and found to be 98-101%. The proposed method was validated and results conformed to ICH parameters.
Cited by 7
Denny Satria, Nazliniwaty Nazliniwaty, Olivia Avriyanti Hanafiah · Research Journal of Pharmacy and Technology · 2025
Suresh Kumar, Nirma Chavda · Asian Journal of Pharmaceutical Analysis · 2023
Parixit Prajapati, Pankaj Kapupara, Hitesh Vekariya · Research Journal of Pharmacy and Technology · 2024
Mahesh Attimarad, Katharigatta Narayanaswamy Venugopala, Muhammad S. Chohan · Molecules · 2022
Pintu Prajapati, Bhumika Prajapati, Veera Shakar Pulusu · Journal of AOAC International · 2023
Pintu Prajapati, Hetal Ahir, Bhumika Prajapati · Journal of AOAC INTERNATIONAL · 2022
Mahesh Attimarad, Katharigatta Narayanaswamy Venugopala, Mohammed Munirul Islam · Indian Journal of Pharmaceutical Education and Research · 2022
Related research
- Real Time Polymerase Chain Reaction versus Enzyme Linked Immunosorbent Assay in the Diagnosis of Cytomegalovirus Infection in Pregnant Women — shares topic coverage
- Home Recording And Video Selection: Their use In A Low-Resource Setting For Epilepsy Diagnosis — shares topic coverage
- Laboratory Performance Evaluation of Wantai HIV 1/2 Rapid Test Kit — shares topic coverage
- Performance of WRF’S Microphysics Options to Increase the Medium Range Rainfall Forecast Accuracy in Tamil Nadu Cauvery Delta Zone — shares topic coverage
- Remote Sensing and GIS Based Crop Acreage Estimation of the Rabi Season Growing Crop of the Middle Gujarat (India) — shares topic coverage
Article metrics
Real usage data collected on this platform.
2
Page views
0
PDF downloads
0
Outbound clicks
7
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.