Development and Validation of Ultra Visible Spectrophotometric Method for the Estimation of Thymoquinone
Sagar Trivedi, Veena Belgamwar, Kamlesh Wadher
Asian Journal of Applied Chemistry Research · pp. 25–30 · Published 21 Apr 2021
10.9734/ajacr/2021/v8i230189Abstract
Aim: The work mainly focuses on developing a robust UV-Visible spectroscopic method for qualitative and quantitative analysis of thymoquinone and this will open to many possibilities in exploiting the wonders of this bioactive molecule. Study Design: UV-vis Spectrophotometric method development for thymoquinone. Place and Duration of Study: Department of Pharmaceutics, Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University Campus, Nagpur, India. Methodology: To find out λmax and validate the developed method using Double beam UV – visible spectrophotometer module (JASCO V-630). Results: The thymoquinone was found to be soluble in methanol. The absorption maximum (λmax) was found to be 252 nm. The good linearity was found to be within concentration range of 2-10 μg/ml with correlation coefficient (r2) >0.99 and regression equation of the curve was found to be y = 0.0173x − 0.0394 at 252 nm. The precision (intra-day and inter-day) data represents good reproducibility with % RSD lower than 2.0% which assured that method is précised. Mean recovery value at different concentrations was found to be higher than 90%, indicates accuracy of the method. LOD and LOQ for thymoquinone were reported and were found to be 0.016 μg/ml and 0.0531μg/ ml 252 nm. Conclusion: The developed method was found to be simple, specific, economic, reliable, accurate, precise, and reproducible used as a quality control tool for analysis of pure thymoquinone and thymoquinone in formulations.
References (6)
- 1 The Neuroprotective Effects of Thymoquinone: A Review [DOI]
- 2 Traditional medicine and gastroprotective crude drugs [DOI]
- 3 Development of UV spectrophotometry methods for concurrent quantification of amlodipine and celecoxib by manipulation of ratio spectra in pure and pharmaceutical formulation [DOI]
- 4 Thymoquinone exhibits anti-inflammatory, antioxidant, and immunomodulatory effects on allergic airway inflammation [DOI]
- 5 The Effect of Nigella Sativa Extract (Thymoquinone) on Glucose Insulin Levels and Body Weight of Induced Diabetic Female Rats [DOI]
- 6 Validation of a UV Spectrometric Method for the Assay of Tolfenamic Acid in Organic Solvents [DOI]
Cited by 15
Sagar Trivedi, Ujban Hussain, Rishabh Agade · Pharmacological Research - Natural Products · 2024
Sagar Trivedi, Manisha Kawadkar, Diksha Pawar · Precision Medication · 2025
Sagar Trivedi, Rishabh Agade, Veena Belgamwar · Journal of Pharmacological and Toxicological Methods · 2024
Sagar Trivedi, Abhidha Deshpande, Veena Belgamwar · Journal of Pharmaceutical Research International · 2021
RISHABH AGADE, UJBAN HUSSAIN, SAGAR TRIVEDI · International Journal of Applied Pharmaceutics · 2024
SUCHITA WAGHMARE, UJBAN MD HUSSAIN, NILESH RAROKAR · International Journal of Applied Pharmaceutics · 2025
Showing 6 of 15 known citations — external sources report more than can currently be individually listed.
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