Skip to content
Research Article Open access CC BY 3.0

Development of Ultrasound Sensitive eLiposomes Containing Doxorubicin for Drug Delivery

Chung-Yin Lin, William G. Pitt, Kuo-Chen Wei

Journal of Pharmaceutical Research International · pp. 2296–2311 · Published 1 Oct 2014

10.9734/BJPR/2014/13143

Abstract

Aims: A novel nanocarrier was formulated by remote loading of doxorubicin (Dox) into a dipalmitoylphosphatidylcholine (DPPC) liposome that also contains various perfluorocarbon (PFC) droplets within its aqueous interior. It was shown that Dox can be loaded to a level of up to 67% into these large unilamellar vesicles composed of DPPC and cholesterol by employing a transmembrane pH gradient technique. Methods: The different encapsulation efficiencies for these eLipoDox constructs of differing PFC composition are 45.5% (PFC5), 31.5% (PFC6) and 66.7% (PFC5/PFC6 mixture, PFCm). At 30 seconds of insonation, the eLipoDox formulation with PFCm droplets appeared to release more Dox than did eLipoDox with pure PFC5 or PFC6 droplets. The thermal stability of these eLipoDox formulations were examined at 37ºC at different times; then controlled delivery was demonstrated by applying low-frequency ultrasound (US) at 1 W/cm2. Results: The eLipoDox with PFC6 or PFCm showed the best combination of thermal stability and drug release. An immunoblotting analysis indicates that ultrasound-triggered Dox release from eLipoDox could provide a higher quantity of nanodrug into tumor cells and thus may have cytostatic effects in cancer cells. Conclusion: These eLipoDox constructs with low boiling point PFCs have the potential to provide more effective ultrasonically activated drug therapy to a desired location.

Doxorubicin controlled drug delivery insonation emulsion liposome eLiposome acoustic droplet vaporization.

References (33)

  1. 1 CRC Handbook of Chemistry and Physics [DOI]
  2. 2 Principles of colloid and surface chemistry
  3. 3 Principles of colloid and surface chemistry [DOI]
  4. 4 Handbook of Chemistry and Physics. [DOI]
  5. 5 Remote loading of doxorubicin into liposomes driven by a transmembrane phosphate gradient [DOI]
  6. 6 Acoustic Droplet Vaporization and Propulsion of Perfluorocarbon‐Loaded Microbullets for Targeted Tissue Penetration and Deformation [DOI]
  7. 7 Drug-loaded nano/microbubbles for combining ultrasonography and targeted chemotherapy [DOI]
  8. 8 Design and Evaluation of Doxorubicin-containing Microbubbles for Ultrasound-triggered Doxorubicin Delivery: Cytotoxicity and Mechanisms Involved [DOI]
  9. 9 Formulation and Acoustic Studies of a New Phase-Shift Agent for Diagnostic and Therapeutic Ultrasound [DOI]
  10. 10 Design of ultrasonically-activatable nanoparticles using low boiling point perfluorocarbons [DOI]
  11. 11 Cytosolic drug delivery using pH- and light-sensitive liposomes [DOI]
  12. 12 Ultrasound sensitive eLiposomes containing doxorubicin for drug targeting therapy [DOI]
  13. 13 Ultrasonic gene and drug delivery using eLiposomes [DOI]
  14. 14 The anti-cancer drug, doxorubicin, causes oxidant stress-induced endothelial dysfunction [DOI]
  15. 15 The Effects of pH and Intraliposomal Buffer Strength on the Rate of Liposome Content Release and Intracellular Drug Delivery [DOI]
  16. 16 Phase change events of volatile liquid perfluorocarbon contrast agents produce unique acoustic signatures [DOI]
  17. 17 Acoustic Droplet Vaporization in Biology and Medicine [DOI]
  18. 18 Acoustic Droplet Vaporization and Propulsion of Perfluorocarbon‐Loaded Microbullets for Targeted Tissue Penetration and Deformation [DOI]
  19. 19 Encapsulating Nanoemulsions Inside eLiposomes for Ultrasonic Drug Delivery [DOI]
  20. 20 Phospholipids as Emulsion Stabilizers. 1. Interfacial Tensions [DOI]
  21. 21 Enhancement of focused ultrasound with microbubbles on the treatments of anticancer nanodrug in mouse tumors [DOI]
  22. 22 Quantitative and qualitative investigation into the impact of focused ultrasound with microbubbles on the triggered release of nanoparticles from vasculature in mouse tumors [DOI]
  23. 23 Phase transitions of perfluorocarbon nanoemulsion induced with ultrasound: A mathematical model [DOI]
  24. 24 Formation of eLiposomes as a drug delivery vehicle [DOI]
  25. 25 Phase transitions of nanoemulsions using ultrasound: Experimental observations [DOI]
  26. 26 Glyceraldehyde 3-Phosphate Dehydrogenase Depletion Induces Cell Cycle Arrest and Resistance to Antimetabolites in Human Carcinoma Cell Lines [DOI]
  27. 27 Ultrasound-Induced Calcein Release From eLiposomes [DOI]
  28. 28 Examination of fluorination effect on physical properties of saturated long-chain alcohols by DSC and Langmuir monolayer [DOI]
  29. 29 Chemiluminescence detection of proteins from single cells. [DOI]
  30. 30 Menadione causes endothelial barrier failure by a direct effect on intracellular thiols, independent of reactive oxidant production [DOI]
  31. 31 Remote Loading of Anthracyclines into Liposomes [DOI]
  32. 32 Cytotoxicity of chemotherapeutic agents in glyceraldehyde-3-phosphate dehydrogenase-depleted human lung carcinoma A549 cells with the accelerated senescence phenotype [DOI]
  33. 33 Safety evaluation of superheated perfluorocarbon nanodroplets for novel phase change type neurological therapeutic agents [DOI]

Cited by 2

Smart ultrasound-triggered doxorubicin-loaded nanoliposomes with improved therapeutic response: A comparative study.

T. Shalaby, Wessam M. El-Refaie, Rasha S. Shams El-Din · Journal of Pharmacy and Science · 2020

Showing 1 of 2 known citations — external sources report more than can currently be individually listed.

Article metrics

Real usage data collected on this platform.

9

Page views

0

PDF downloads

3

Outbound clicks

2

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.