Effect of Dietary Lysine and DL-Methionine Supplementation in Roasted Soyabean-Based Diet on the Growth Performance and Blood Profiles of Clarias gariepinus
Asian Journal of Fisheries and Aquatic Research · pp. 14–26 · Published 30 Jul 2024
10.9734/ajfar/2024/v26i8791Abstract
This study was designed to investigating the growth performance, hematology, and serum biochemistry of Clarias gariepinus nourished with roasted soyabean based diets with varied inclusion of dietary amino acid. A total of 360 Clarias gariepinus juveniles, aged eight weeks and weighing 19.0±0.4 grams, were randomly assigned to six different diets with varying inclusion levels of lysine and DL-methionine (g/100g). The diets were as follows: RS1 (Control) without lysine or DL-methionine supplementation; RS 2 - 0g + 1g; RS 3 - 0.25g + 0.75g; RS 4 - 0.5g + 0.5g; RS 5 - 0.75g + 0.25g; and RS 6 - 1g + 0g of Lysine and DL-methionine. For 84 days, the fish were fed until they were completely satisfied. Each treatment was in triplicate. Supplementing lysine and methionine in a diet based on roasted soybeans did not significantly (P>0.05) effect on the growth parameter such as final weight, feed conversion ratio, and/or specific growth rate in fish. A significantly higher survival rate was observed in fish on diet RS 5 (85.60±0.60) and was closely followed by fish fed diet RS 3 and RS 4. Fish fed diets RS 4 and RS 5 exhibited significantly higher (P<0.05) packed cell volume (PCV %) and hemoglobin levels. While, White blood cell counts (x10^9/L) varied significantly among the different diets. The addition of supplemental amino acids did not significantly (P>0.05) affect total protein (g/L), and the values ranges from 6.60±0.52 (RS 6) to 7.87±0.55 (RS 4). This study showed that supplementation of lysine and DL-methionine in Roasted Soyabean based diet could improve growth performance of fish with no adverse effect on fish health.
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