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Research Article Open access CC BY 4.0

Evaluating CRISPR/Cas9's Progressive Role in Crop Enhancement and Sustainable Agriculture

Akhil Sathiyabalan, Mohammad Amir, Rubby Sandhu, Sumona Bhattacharya

Journal of Advances in Biology & Biotechnology · pp. 855–867 · Published 31 May 2024

10.9734/jabb/2024/v27i6949

Abstract

This review paper provides a comprehensive exploration of the progressive effect of CRISPR/Cas9 genome altering innovation on functional genomics and crop enhancement. It highlights the evolution of CRISPR technology, emphasizing its versatile applications in plant breeding. The paper discusses the transition from gene knockouts to precise modifications and multiplex genome engineering, with a focus on enhancing plant nutrition, disease resistance, and drought tolerance. Traditional Cas9-guide RNA delivery methods are compared with ascending CRISPR ribonucleoproteins (RNPs) as solutions to overcome limitations associated with plasmid-based systems. The booming worldwide claim for food crops underscores the possibility of CRISPR/Cas9-based genome editing for improving cereal features. Earlier innovations like zinc finger nucleases (ZFNs) and TALENs, CRISPR/Cas9 offers a more cost-effective and time-efficient approach. It enables the blueprint and cloning of numerous gRNAs for accurate genome targeting, with enhanced specificity and efficiency achieved through modified Cas9 cassettes and enzymes from various bacterial species. The review also emphasizes CRISPR/Cas9's potential to develop non-genetically modified (Non-GMO) crops with certain needed traits, thereby boosting yield potential under various stress conditions. Furthermore, the article discusses problems and solutions analogous with CRISPR/Cas9-based genome editing, providing valuable insights for researchers in crop advancement.

Genome editing ribonucleoproteins Cas9-gRNA ZFNs TALENs Non-GMO

References (55)

  1. 1 The new frontier of genome engineering with CRISPR-Cas9 [DOI]
  2. 2 Nucleotide sequence of the iap gene, responsible for alkaline phosphatase isozyme conversion in Escherichia coli, and identification of the gene product [DOI]
  3. 3 Targeting DNA Double-Strand Breaks with TAL Effector Nucleases [DOI]
  4. 4 CRISPR/Cas Genome Editing and Precision Plant Breeding in Agriculture [DOI]
  5. 5 Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis, tobacco, sorghum and rice [DOI]
  6. 6 Targeted Chromosomal Cleavage and Mutagenesis in Drosophila Using Zinc-Finger Nucleases [DOI]
  7. 7 Efficient targeted multiallelic mutagenesis in tetraploid potato (Solanum tuberosum) by transient CRISPR-Cas9 expression in protoplasts [DOI]
  8. 8 Editing plant genomes with CRISPR/Cas9 [DOI]
  9. 9 Genome editing in potato via CRISPR‐Cas9 ribonucleoprotein delivery [DOI]
  10. 10 A deletion mutation in TaHRC confers Fhb1 resistance to Fusarium head blight in wheat [DOI]
  11. 11 Deployment of new biotechnologies in plant breeding [DOI]
  12. 12 Rapid improvement of grain weight via highly efficient CRISPR/Cas9-mediated multiplex genome editing in rice [DOI]
  13. 13 Targeted Mutagenesis of Duplicated Genes in Soybean with Zinc-Finger Nucleases [DOI]
  14. 14 CRISPR/Cas9-mediated mutagenesis of the RIN locus that regulates tomato fruit ripening [DOI]
  15. 15 Engineering abiotic stress tolerance via CRISPR/ Cas-mediated genome editing [DOI]
  16. 16 Engineering crops of the future: CRISPR approaches to develop climate-resilient and disease-resistant plants [DOI]
  17. 17 Application of CRISPR/Cas9 in Crop Quality Improvement [DOI]
  18. 18 CRISPR–Cas9: A History of Its Discovery and Ethical Considerations of Its Use in Genome Editing [DOI]
  19. 19 Anti-CRISPRs: Protein Inhibitors of CRISPR-Cas Systems [DOI]
  20. 20 TALE and TALEN genome editing technologies [DOI]
  21. 21 Integrated Genomic Selection for Accelerating Breeding Programs of Climate-Smart Cereals [DOI]
  22. 22 Mutagenesis of the FAE1 genes significantly changes fatty acid composition in seeds of Camelina sativa [DOI]
  23. 23 Advancements in molecular marker technologies and their applications in diversity studies [DOI]
  24. 24 Mutation Breeding in Tomato: Advances, Applicability and Challenges [DOI]
  25. 25 A Revolution toward Gene-Editing Technology and Its Application to Crop Improvement [DOI]
  26. 26 CRISPR-Cas9 System for Plant Genome Editing: Current Approaches and Emerging Developments [DOI]
  27. 27 RNA Interference and CRISPR/Cas Gene Editing for Crop Improvement: Paradigm Shift towards Sustainable Agriculture [DOI]
  28. 28 CRISPR-based antimicrobials to obstruct antibiotic-resistant and pathogenic bacteria [DOI]
  29. 29 Genome editing and beyond: what does it mean for the future of plant breeding? [DOI]
  30. 30 Genome editing for resistance against plant pests and pathogens [DOI]
  31. 31 Generation of low-cadmium rice germplasms via knockout of OsLCD using CRISPR/Cas9 [DOI]
  32. 32 CRISPR-Based Genome Editing: Advancements and Opportunities for Rice Improvement [DOI]
  33. 33 Identifying a large number of high-yield genes in rice by pedigree analysis, whole-genome sequencing, and CRISPR-Cas9 gene knockout [DOI]
  34. 34 Mutational Breeding and Genetic Engineering in the Development of High Grain Protein Content [DOI]
  35. 35 Targeted gene disruption coupled with metabolic screen approach to uncover the LEAFY COTYLEDON1-LIKE4 (L1L4) function in tomato fruit metabolism [DOI]
  36. 36 CRISPR-based strategies for targeted transgene knock-in and gene correction [DOI]
  37. 37 Strategies for Enhancing the Homology-Directed Repair Efficiency of CRISPR-Cas Systems [DOI]
  38. 38 Heavily Armed Ancestors: CRISPR Immunity and Applications in Archaea with a Comparative Analysis of CRISPR Types in Sulfolobales [DOI]
  39. 39 Production of Herbicide-Sensitive Strain to Prevent Volunteer Rice Infestation Using a CRISPR-Cas9 Cytidine Deaminase Fusion [DOI]
  40. 40 Inheritance Studies in Segregating Population of Bread Wheat (Triticum aestivum L.) [DOI]
  41. 41 Analysis of Heterotic Potential for Yield and Its Contributing Traits in Wheat (Triticum aestivum L.) [DOI]
  42. 42 CRISPR/Cas9-mediated knockout of the DCL2 and DCL4 genes in Nicotiana benthamiana and its productivity of recombinant proteins [DOI]
  43. 43 The Code Breaker: Jennifer Doudna, Gene Editing, and the Future of the Human Race [DOI]
  44. 44 Bio-informatic analysis of CRISPR protospacer adjacent motifs (PAMs) in T4 genome [DOI]
  45. 45 CRISPR Screens in Plants: Approaches, Guidelines, and Future Prospects [DOI]
  46. 46 Strategies for CRISPR/Cas9-mediated genome editing: from delivery to production of modified plants [DOI]
  47. 47 The CRISPR-Cas Immune System and Genetic Transfers: Reaching an Equilibrium [DOI]
  48. 48 Simultaneous CRISPR/Cas9-mediated editing of cassava eIF4E isoforms nCBP-1 and nCBP-2 reduces cassava brown streak disease symptom severity and incidence [DOI]
  49. 49 The pAblo·pCasso self-curing vector toolset for unconstrained cytidine and adenine base-editing in Gram-negative bacteria [DOI]
  50. 50 Development of blast resistant rice plants using CRISPR / Cas9 system for genome editing [DOI]
  51. 51 GENOMIC APPROACHES FOR IMPROVING DROUGHT TOLERANCE IN WHEAT (TRITICUM AESTIVUM L.): A COMPREHENSIVE REVIEW [DOI]
  52. 52 Productivity of Fodder Maize (Zea mays L.) SFM-1 under Varied Sowing Dates and Nitrogen Levels [DOI]
  53. 53 ENHANCING SEED QUALITY AND INSECT MANAGEMENT IN WHEAT (TRITICUM AESTIVUM L.) THROUGH OPTIMIZATION OF STORAGE TREATMENTS WITH NATURAL AND CHEMICAL COMPOUNDS [DOI]
  54. 54 Identification of Induced Chemical Mutants for Improving Yield and its Attributing Traits in Wheat (Triticum aestivum L.) against Changing Climate [DOI]
  55. 55 Biochemical Analysis of SBEIIb Mutant Rice Lines Generated through CRISPR/Cas-mediated Targeted Mutagenesis [DOI]

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