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Research Article Open access CC BY 4.0

Evaluation of in vitro Anti-proliferative Activity of L-arginine deiminase from Novel Marine Bacterial Isolate

Rahamat Unissa, M. Sudhakar, A. Sunil Kumar Reddy

Microbiology Research Journal International · pp. 1–10 · Published 14 Mar 2016

10.9734/BMRJ/2016/23592

Abstract

L-Arginine deiminase is a therapeutic l-arginine depleter found to counteract various arginine auxotrophic cancer cells (do not express ASS/OCT). The aim of the present study was to evaluate the anti -proliferative activity of the purified l-arginine deiminase from Vibrio alginolyticus 1374.  Production of the enzyme was carried out by shake flask method under optimal conditions. The enzyme thus produced was purified to near homogeneity by ammonium sulphate fractionation followed by ion exchange and gel filtration chromatography. The enzyme was purified to 529.43 fold and showed final specific activity of 280.6 IU/mg with 43.5% yield. SDS-PAGE revealed that the purified enzyme had a molecular weight of 48 kDa. The purified enzyme tested against A375-C6, MCF-7, HCT-113 and Jurkat, clone E6-1cell lines showed I.C.50 value of about 5. 21, 6.3,8 and 3.13 U/ml respectively. ADI obtained from Vibrio alginolyticus 1374 has several beneficial properties needed for its use as a therapeutic and industrial enzyme. It was active over wide range of pH (4-10) and temperature (25°-50°C), had high substrate specificity etc. Moreover it showed broad range of activity against cancer cells lines of human origin. Further animal model studies must be carried out to develop ADI as a potential drug.

Vibrio alginolyticus 1374 ornithine carbamoyl transferase argininosuccinate synthetase A375-C6 MCF-7 HCT-113 and jurkat clone E6-1cell lines

References (22)

  1. 1 PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT [DOI]
  2. 2 Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 [DOI]
  3. 3 Arginine metabolism: nitric oxide and beyond [DOI]
  4. 4 Purification, Characterization, and Sequence Analysis of a Potential Virulence Factor from Porphyromonas gingivalis , Peptidylarginine Deiminase [DOI]
  5. 5 D IET AND A POPTOSIS [DOI]
  6. 6 Flavonoids as RTK inhibitors and potential anticancer agents [DOI]
  7. 7 Anti‐tumor Activity of Arginine Deiminase from Mycoplasma arginini and Its Growth‐inhibitory Mechanism [DOI]
  8. 8 Purification, immobilization, and biochemical characterization of l ‐arginine deiminase from thermophilic Aspergillus fumigatus KJ 434941: Anticancer activity in vitro [DOI]
  9. 9 Effect of dietary arginine restriction upon ornithine and polyamine metabolism during two-stage epidermal carcinogenesis in the mouse.
  10. 10 Single Amino Acid (Arginine) Restriction: Growth and Death of Cultured HeLa and Human Diploid Fibroblasts [DOI]
  11. 11 Crystallization and properties of L-arginine deiminase of Pseudomonas putida. [DOI]
  12. 12 Vincristine could partly suppress stromal support to T-ALL blasts during pegylated arginase I treatment [DOI]
  13. 13 [92] Isolation and determination of arginine and citrulline [DOI]
  14. 14 Anti-inflammatory effects of recombinant arginine deiminase originating from Lactococcus lactis ssp. lactis ATCC 7962.
  15. 15 Arginine deiminase from Mycoplasma arthritidis. Evidence for multiple forms. [DOI]
  16. 16 Identification and purification of arginine deiminase that originated from Mycoplasma arginini [DOI]
  17. 17 The Cranberry-Promising Health Benefits, Old and New [DOI]
  18. 18 Caveat: mycoplasma arginine deiminase masquerading as nitric oxide synthase in cell cultures [DOI]
  19. 19 Purification and Some Properties of Arginine Deiminase inEuglena gracilisZ [DOI]
  20. 20 Condition Optimization and Production of Extracellular l-Arginine Deiminase from Vibrio Alginolyticus 1374 [DOI]
  21. 21 Extraction, Purification and Characterization (A) of Arginine Deiminase Enzyme from a Local Higher Productive Isolation of Enterococcus faecium M1
  22. 22 Optimization of Arginine deiminase production from a local higher productive isolate Enterococcus faecium M1 [DOI]

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