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Research Article Open access CC BY 4.0

Evaluation of Phytoremediation, Physicochemical and Heavy Metal Assessment of Crude Oil Polluted Soil Using Pseudomonas and Bacillus spp, Rivers State, Nigeria

Jaja, Soba Emmanuel

Journal of Advances in Microbiology · pp. 10–22 · Published 3 Nov 2023

10.9734/jamb/2023/v23i11768

Abstract

Crude oil exploration has been beneficial to our economy but detrimental to our environment with the artisanal `refineries further compounding the challenge. This research was aimed at investigating the phytoremediation, physicochemical and heavy metal assessment of crude oil polluted soil using Peudomonas spp. and Bacillus spp. as augmenting microorganism, in Rivers state, Nigeria. This study was carried out in in south-south Nigeria (B-dere in Gokana Local government of `Rivers State). Contaminated Soil were collected and subjected to standard microbiological methods. Contaminated Soil samples were collected from two different area of Rivers state with sterilized soil auger at two depths of 0-15cm and 15-30cm. The contaminated soil sampled were analyzed for heavy metal (Cadium Chromium, Lead and Zinc) using Atomic Absorption spectrophotometric method. Microbiological analysis was carried out on the soil samples. Ten (10) treatments consisiting of contaminated soil (CS), uncontaminated soil (US), uncontaminated soil(US), Panicum subalbidum and Schoenoplectus senegalensis were setup for a period of 28 days. Physicochemical parameters were analyzed for uncontaminated soil and contaminated soil. The physicochemical parameters analyzed were pH, Temperature, Nitrogen, Phosphorus, Potassium and Total Hydrocarbon Content. The physiochemical parameters of the uncontaminated soil  were pH (5.43), temperature (27oC), Electrical conductivity (9uS/cm), Moisture content (7.80%), Total organic carbon (0.93%), Soil organic matter (1.60%), total Nitrogen (56.695mg/kg), available phosphorus (0.621mg/kg), potassium (7.125mg/kg) and total hydrocarbon content (700mg/kg). Results revealed that the amount of hydrocarbon remediated and percentage (%) bioremediation in the soil after 28 days of monitoring from the initial THC value of (4050 mg/kg), is higher in set up with CS+PSE+SCH (3454mg/kg; 85.28%) and lowest in set up with US+SCH (434mg/kg: 62%) and the amount uptake of phytoremediation in the root after 28 days of monitoring to be higher in set up with CS+BAC+SCH (632Mg/kg; 15.6%) and lowest in set up with US+SCH (12.2mg/kg; 1.74%). The heavy metals assessed were cadium (Cd), chromium (Cr), lead (Pb) and zinc (Zn). This research revealed and recommend that Schoenoplectus senegalensis as a suitable plant species for phytoremediation of crude oil contaminated soil. Hydrocarbon utilizing bacteria identified in this study were; Pseudomonas and Bacilus spp.

Crude oil Bacillus Peudomonas soil sampled biosurfactants hydrocarbons biodegradation microbial diversity soil ecotoxicity remediation soil pollution oil contamination

References (22)

  1. 1 Anaerobic Microbial Degradation of Hydrocarbons: From Enzymatic Reactions to the Environment [DOI]
  2. 2 Enhanced bioremediation of n-alkane in petroleum sludge using bacterial consortium amended with rhamnolipid and micronutrients [DOI]
  3. 3 Phytoremediation of petroleum contaminated soils by Mirabilis Jalapa L. in a greenhouse plot experiment [DOI]
  4. 4 Bioaugmentation as a soil bioremediation approach [DOI]
  5. 5 Biosurfactant and Degradative Enzymes Mediated Crude Oil Degradation by Bacterium Bacillus subtilis A1 [DOI]
  6. 6 Biostimulation and bioaugmentation of native microbial community accelerated bioremediation of oil refinery sludge [DOI]
  7. 7 Hydrocarbon‐degrading bacteria: the oil‐spill clean‐up crew [DOI]
  8. 8 Phytoremediation of an aged petroleum contaminated soil using endophyte infected and non-infected grasses [DOI]
  9. 9 Development of bioreactors for comparative study of natural attenuation, biostimulation, and bioaugmentation of petroleum-hydrocarbon contaminated soil [DOI]
  10. 10 Plant–bacteria interactions in the removal of pollutants [DOI]
  11. 11 Insights into microbial communities mediating the bioremediation of hydrocarbon-contaminated soil from an Alpine former military site [DOI]
  12. 12 Potential Approaches to Improving Biodegradation of Hydrocarbons for Bioremediation of Crude Oil Pollution [DOI]
  13. 13 Use of solvent extraction to remediate soils contaminated with hydrocarbons [DOI]
  14. 14 Comparison of the phytoremediation potentials of Medicago falcata L. And Medicago sativa L. in aged oil-sludge-contaminated soil [DOI]
  15. 15 Microbial diversity and activity of an aged soil contaminated by polycyclic aromatic hydrocarbons [DOI]
  16. 16 Potentialities of Six Plant Species on Phytoremediation Attempts of Fuel Oil-Contaminated Soils [DOI]
  17. 17 Production of Biosurfactants by Hydrocarbons degrading bacteria isolated from Soummam watershed Sediments of Bejaia in Algeria [DOI]
  18. 18 Compositional Changes of Hydrocarbons of Residual Oil in Contaminated Soil During Ozonation [DOI]
  19. 19 Applied and Environmental Microbiology [DOI]
  20. 20 Applied Microbiology and Biotechnology [DOI]
  21. 21 Journal of Molecular Microbiology and Biotechnology [DOI]
  22. 22 Bioprocess and Biosystems Engineering [DOI]

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