Extraction, Phytochemical Screening and Antioxidant Potential of Ethanolic Extract of Drypetes roxburghii

Abstract

Humans are affected by free radicals in over a hundred different ways. Synthetic antioxidants have been linked to harmful health consequences, prompting substantial investigation into naturally occurring antioxidants, particularly those found in plants. The goal of this study was to determine the phytochemical components and in vitro antioxidant activity of Drypetes roxburghii (D. roxburghii, Euphorbiaceae) leaves collected in the Bhopal district of Madhya Pradesh. The 1, 1-diphenyl-2-picrylhydrazyl radical (DPPH) test was used to measure antioxidant activity. The presence of cardiac glycosides, flavonoids, phenol, proteins, carbohydrate, and saponins in the ethanolic extracts of D. roxburghii leaves was discovered using phytochemical screening. The ethanolic and aqueous extracts of D. roxburghii leaves yielded 1.9 and 2.6 percent w/w, respectively. The ethanolic extract of D. roxburghii leaves contained 1.48 and 0.70 mg/100 mg of total phenolic and flavonoids, respectively. When comparing the DPPH scavenging activity of leaf ethanolic extract to Ascorbic acid (standard), the IC50 value was 87.80. It suggests that the plant has the ability to scavenge free radicals, and it might lead to new discoveries in the continuing hunt for natural antioxidants in medicinal plants that can be utilised to treat illnesses caused by free radical reactions.