Formulation and Characterization of Nystatin-loaded Nanostructured Lipid Carriers for Topical Delivery against Cutaneous Candidiasis
Rawia M. Khalil, A. Abd-Elbary, Mahfouz A. Kassem, Mohamed S. El Ridy, Mona M. Abou Samra, Ghada E. A. Awad, Soheir S. Mansy
Journal of Pharmaceutical Research International · pp. 490–512 · Published 24 Dec 2013
10.9734/BJPR/2014/7055Abstract
Aims: The objective of the current study was to formulate nystatin (Nyst) into nanostructured lipid carriers (NLCs) to enhance its antifungal activity. Place and Duration of Study: Department of pharmaceutical technology, national research centre, Egypt, between mars 2011 to april 2013 Methodology: Nyst loaded NLCs (NYST-NLCs) were prepared by the hot homogenization and ultrasonication method followed by evaluation of its topical effect on the cutaneous candidiasis. The prepared Nyst-NLCs were characterized for entrapment efficiency, particle size, zeta potential, morphology (transmission electron microscopy), thermal characterisation (differential scanning calorimetry) and in vitro drug release. The study design involves the investigation of the effect of three independent variables namely liquid lipid type (Miglyol 812 and Squalene), liquid lipid concentration (30 and 50%) and Nyst concentration (0.125 and 0.25%). A stability study for 6 months was performed. A microbiological study was conducted in male rats infected with Candida albicans. Results: NLC dispersions were spherical in shape with particle size ranging from 68.06±6.56 to 141.8±3.33 nm. The entrapment efficiencies ranged from 45.50±2.34 to 92.73±0.33% with zeta potential ranging from -26.2 to -39.2 mV. The stability studies done for 6 months indicated that Nyst-NLCs were stable for more than 6 monthes.the microbiological studies showed A least number of colonies forming units (cfu/ml) were recorded for the selected Nyst-NLCs compared to the drug solution and the Nystatin® cream present in the market. Conclusion: It can be fulfilled from this work that NLCs represent promising carrier for topical delivery of Nyst offering good physical stability, high entrapment efficiency and controlled drug release. Nyst-NLCs are a good candidate for cutaneous treatment of fungal infection.
References (28)
- 1 Solid lipid nanoparticles (SLN) for controlled drug delivery – a review of the state of the art [DOI]
- 2 Solid lipid nanoparticles Production, characterization and applications [DOI]
- 3 Solid lipid nanoparticles (SLN) and nanostructured lipid carriers (NLC) in cosmetic and dermatological preparations [DOI]
- 4 Development of a controlled release formulation based on SLN and NLC for topical clotrimazole delivery [DOI]
- 5 Analysis of Data on the Medicament Release from Ointments [DOI]
- 6 Are nanostructured lipid carriers (NLCs) better than solid lipid nanoparticles (SLNs): Development, characterizations and comparative evaluations of clotrimazole-loaded SLNs and NLCs? [DOI]
- 7 Lipid nanoparticles as vehicles for topical psoralen delivery: Solid lipid nanoparticles (SLN) versus nanostructured lipid carriers (NLC) [DOI]
- 8 Body distribution in mice of intravenously injected camptothecin solid lipid nanoparticles and targeting effect on brain [DOI]
- 9 SOLID LIPID NANOPARTICLES: A REVIEW
- 10 Superior Penetration and Retention Behavior of 50 nm Gold Nanoparticles in Tumors [DOI]
- 11 Effects of lipophilic emulsifiers on the oral administration of lovastatin from nanostructured lipid carriers: Physicochemical characterization and pharmacokinetics [DOI]
- 12 Development and optimization of solid lipid nanoparticle formulation for ophthalmic delivery of chloramphenicol using a Box-Behnken design [DOI]
- 13 Lipid nanoparticles for prolonged topical delivery: An in vitro and in vivo investigation [DOI]
- 14 Formulation, characterization and pulmonary deposition of nebulized celecoxib encapsulated nanostructured lipid carriers [DOI]
- 15 Diazepam-Loaded Solid Lipid Nanoparticles: Design and Characterization [DOI]
- 16 Vaginal candidosis: epidemiological and etiological factors [DOI]
- 17 Formulation and Evaluation of Solid Lipid Nanoparticles of Ramipril [DOI]
- 18 Studies on oral absorption of stearic acid SLN by a novel fluorometric method [DOI]
- 19 Development, characterization and in vivo assessment of effective lipidic nanoparticles for dermal delivery of fluconazole against cutaneous candidiasis [DOI]
- 20 Nanostructured Lipid Carriers (NLC)-Based Gel for Topical Delivery of Aceclofenac: Preparation, Characterization and In Vivo Evaluation [DOI]
- 21 Some histological methods: Trichrome staining and their preliminary technique
- 22 Glucocorticoid entrapment into lipid carriers — characterisation by parelectric spectroscopy and influence on dermal uptake [DOI]
- 23 Antifungal activity of silver nanoparticles in combination with nystatin and chlorhexidine digluconate against C andida albicans and C andida glabrata biofilms [DOI]
- 24 Nanostructured lipid carriers (NLCs) versus solid lipid nanoparticles (SLNs) for topical delivery of meloxicam [DOI]
- 25 Chitosan-associated SLN:in vitroandex vivocharacterization of cyclosporine A loaded ophthalmic systems [DOI]
- 26 Formulation and characterization of nystatin gel.
- 27 Novel self-assembled amphiphilic poly(ε-caprolactone)-grafted-poly(vinyl alcohol) nanoparticles: hydrophobic and hydrophilic drugs carrier nanoparticles [DOI]
- 28 A novel model of cutaneous candidiasis produced in prednisolone-treated guinea-pigs [DOI]
Cited by 49
Bharti Gaba, Mohammad Fazil, S. Khan · 2015
S. Kokare, Shubham V. Khot, O. Daware · Next Nanotechnology · 2025
Andrei Vinícius Englert, C. M. Verdi, R. C. V. Santos · Brazilian Archives of Biology and Technology · 2020
Nikita Nandvikar, R. Lala, Amol A. Shinde · 2019
Nayyer Karimi, B. Ghanbarzadeh, H. Hamishehkar · 2018
Nidhi Bais, Anand Birthare, A. Dubey · 2016
Showing 26 of 49 known citations — external sources report more than can currently be individually listed.
Related research
- Knowledge, Attitude and Predisposing Risk Factors of Non-Albican Vulvovaginal Candidiasis among Symptomatic Women in Port Harcourt — shares topic coverage
- Aetiologic Agents of Vaginitis in Sexually Active Women in a Traditional Sprawling Town in the Niger Delta, South – South Nigeria — shares topic coverage
- The Prevalence of Candida albicans and Trichomonas vaginalis among First Year Female Students of a Tertiary Institution in Southeastern Nigeria — shares topic coverage
- Biodegradation of Palm Oil Mill Effluent (POME) and Lipase Activity by Pseudomonas aeruginosa, Bacillus subtilis and Candida albicans — shares topic coverage
- Characterization of Vaginal Lactobacillus Strains and Their Potential Antagonistic Effects on Candida albicans — shares topic coverage
Article metrics
Real usage data collected on this platform.
26
Page views
0
PDF downloads
7
Outbound clicks
49
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.