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Research Article Open access CC BY 4.0

Gliricidia sepium Aqueous Leaf Extract Possesses Antisickling Property

T. Oduola, M. K. Dallatu, A. O. Muhammed, M. A. Ndakotsu, I. M. Adebisi, S. W. Hassan

International Blood Research & Reviews · pp. 1–6 · Published 14 Apr 2016

10.9734/IBRR/2016/25181

Abstract

Background: Extracts of leaf, flower and bark of Gliricidia sepium have been used in the treatment of patients with pathogenic bacterial infections, skin diseases, nematodes and antioxidants disturbances. We recently had information of its use in the treatment of sickle cell disease in some parts of Nigeria. Aim: The aim of this study is to investigate the in-vitro antisickling properties of Gliricidia sepium aqueous leaf extract. Place and Duration of the Study: This study was carried out in the Department of Chemical Pathology, Faculty of Medical Laboratory Sciences, Usmanu Danfodiyo University, Sokoto, Nigeria. Methods: Air-dried leaves were ground and an aliquot, 100 g was extracted by maceration in 1.0 L of distilled water as solvent for 72 h with periodic stirring. The same procedure was repeated with residues and the mixture was filtered and concentrated to dryness. Different concentrations (5 mg, 10 mg, 15 mg, 20 mg, 25 mg, 30 mg, 35 mg, 40 mg, 45 mg and 50 mg) were made from the extract and then tested for their antisickling activities against haemoglobin SS blood using freshly prepared 2% sodium metabisulphite. Results: Our findings revealed strong antisickling activity; 20% antisckling at 5 mg, 50% at 10 mg, 80% at 15 mg and 100% antisickling from 20 mg upward for the leaf extract. Conclusion: It has been shown for the first time, from this study that aqueous extract of Gliricidia sepium has strong antisickling properties justifying its use by the herbalists in the management of sickle cell disease. Toxicity studies, isolation and characterization of active compound are on-going in our laboratories.      

Gliricidia sepium aqueous extract antisickling herbalist

References (15)

  1. 1 Screening for antimicrobial activity of ten medicinal plants used in Colombian folkloric medicine: A possible alternative in the treatment of non-nosocomial infections [DOI]
  2. 2 Vaso-occlusion in sickle cell disease: pathophysiology and novel targeted therapies [DOI]
  3. 3 Mortality in Sickle Cell Anemia in Africa: A Prospective Cohort Study in Tanzania [DOI]
  4. 4 Plants used in guatemala for the treatment of dermatophytic infections. 1. Screening for antimycotic activity of 44 plant extracts [DOI]
  5. 5 The in vitro antisickling and antioxidant effects of aqueous extracts Zanthoxyllum heitzii on sickle cell disorder [DOI]
  6. 6 Antisickling activity of butyl stearate isolated from Ocimum basilicum (Lamiaceae) [DOI]
  7. 7 Antisickling Activity and Membrane Stabilizing Effect of Anthocyanins Extracts from Adansonia digitata L. Barks on Sickle Blood Cells [DOI]
  8. 8 Niprisan (Nix‐0699) improves the survival rates of transgenic sickle cell mice under acute severe hypoxic conditions [DOI]
  9. 9 Antisickling agent in an extract of unripe pawpaw (Carica papaya): Is it real? [DOI]
  10. 10 Molecular mechanisms underlying synergistic adhesion of sickle red blood cells by hypoxia and low nitric oxide bioavailability [DOI]
  11. 11 Antibacterial, Phytochemical and Antioxidant Activities of the Leaf Extracts of Gliricidia sepium and Spathodea campanulata
  12. 12 Studies on the Constituents of Gliricidia sepium (Leguminosae) Leaves and Roots: Isolation and Structure Elucidation of New Triterpenoid Saponins and Aromatic Compounds [DOI]
  13. 13 The Reliability of Sickling and Solubility Tests and Peripheral Blood Film Method for Sickle Cell Disease Screening at District Health Centers in Uganda [DOI]
  14. 14 EVALUATION OF ANTIBACTERIAL ACTIVITY OF THE BARK, FLOWER AND LEAF EXTRACTS OF GLIRICIDIA SEPIUM FROM SOUTH INDIA
  15. 15 In vivored blood cell sickling and mechanism of recovery in whiting,Merlangius merlangus [DOI]

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