Skip to content
Research Article Open access CC BY 4.0

Heat-shock Technique for Isolation of Soil Bacillus Species with Potential of Antibiotics Secretion in Saudi Arabia

N. A. Al-Humam

Microbiology Research Journal International · pp. 1–6 · Published 25 Oct 2016

10.9734/BMRJ/2016/29126

Abstract

The present research aimed to isolate soil Bacillus spp. from Al-Ahsa Province, Kingdom of Saudi Arabia, and test their potential of antibiotics production. Out of 21 soil samples, 34 bacterial isolates were obtained and following identification tests, 26 bacterial isolates in an isolation rate of 76.5% were identified as Bacillus spp by conventional methods and commercial automated identification system.  Identified species were Bacillus subtilis (7 strains), B. polymyxa (4 strains), B. licheniformis (4 strains), B. cereus (3 strains), B. mycoides (3 strains), B. pumilus (1 strains), and Bacillus spp. (4 strains). Disk diffusion test was employed to test the metabolites in sterilized cell free supernatant of isolates against some selected pathogenic bacterial species. The test indicated that B. polymyxa, B. licheniformis, B. cereus, B. mycoides and to a lesser degreeB. subtilis produced promising anti-microbial effects on tested bacterial species comparable to standard antibiotic disk. The study suggests that some of Bacillus spp from the study area have potential to produce antibiotics that may be used to control microbial growth.

Antibiotics soil heat treatment Bacillus species gram-positive Saudi Arabia

References (18)

  1. 1 On the Antibacterial Action of Cultures of a Penicillium, with Special Reference to their Use in the Isolation of B. influenzæ
  2. 2 Color Atlas and Textbook of Diagnostic Microbiology. [DOI]
  3. 3 Bacillus subtilis and other gram-positive bacteria : biochemistry, physiology, and molecular genetics
  4. 4 Ribosomally synthesized antimicrobial peptides: their function, structure, biogenesis, and mechanism of action [DOI]
  5. 5 Antimicrobial activities of some Bacillus spp. strains isolated from the soil [DOI]
  6. 6 Recurrent Challenges for Clinicians: Emergence of Methicillin-resistant Staphylococcus aureus, Vancomycin Resistance, and Current Treatment Options [DOI]
  7. 7 Improvements on colony morphology identification towards bacterial profiling [DOI]
  8. 8 Automated systems for identification of microorganisms [DOI]
  9. 9 Lipopeptides Produced by a Soil Bacillus Megaterium Strain [DOI]
  10. 10 Isolation and Identification ofBacillusSpecies From Soil and Evaluation of Their Antibacterial Properties [DOI]
  11. 11 What's new on the antimicrobial horizon? [DOI]
  12. 12 Determination of Some Properties of Bacillus Isolated from Soil
  13. 13 Purification and characterization of a new peptide antibiotic produced by a thermotolerant Bacillus licheniformis strain [DOI]
  14. 14 [Antimicrobial resistance in developing countries].
  15. 15 Detection and characterization of cerein 7, a new bacteriocin produced by Bacillus cereus with a broad spectrum of activity [DOI]
  16. 16 Evaluation of the VITEK2 BCL card for identification of Bacillus species and other aerobic endosporeformers [DOI]
  17. 17 Automated systems for identification of microorganisms. [DOI]
  18. 18 Identification of 4″-isovaleryl-spiramycin III produced by Bacillus sp. fmbJ [DOI]

Cited by 12

Improving the nutritional value and bioactivity of soybean meal in solid-state fermentation using Bacillus strains newly isolated from the gut of the termite Termes propinquus

Maneeploy Nualkul, Bundit Yuangsoi, Yuichi Hongoh · FEMS Microbiology Letters · 2022

Showing 11 of 12 known citations — external sources report more than can currently be individually listed.

Related research

Article metrics

Real usage data collected on this platform.

89

Page views

0

PDF downloads

19

Outbound clicks

12

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.