Heavy Metals Effect on the Activity and Kinetics of Peroxidase Enzyme in Crude Extracts of Rosmarinus officinalis and Eruca sativa
Omar M. Atrooz, Manar A. Al-Btoush, Ibrahim M. Al-Rawashdeh
International Journal of Biochemistry Research & Review · pp. 1–8 · Published 7 Jan 2017
10.9734/IJBCRR/2016/30399Abstract
The catalytic activities and kinetic parameters of the enzyme peroxidase (POX), extracted from two plant species namely Rosmarinus officinalis and Eruca sativa, were investigated in the presence and absence of various heavy metals. The activation effects of heavy metals Co2+, Fe3+ and Pb2+ have performed a noncompetitive inhibition on the enzyme activity in the crude extract of Eruca sativa. In contrast, Cd2+, Ni+ and Cu2+ have an uncompetitive inhibition for the two selected plants. Furthermore, Fe3+, Al3+, Pb2+ and Mo2+ were found to be acted as noncompetitive inhibitors on the enzyme activity in the crude extract of Rosmarinus officinalis. Presence of heavy metals altered the enzyme activity by acting as uncompetitive or noncompetitive inhibitors depending on the type of heavy metals.
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