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HPLC-MS Evaluation of Mannuronic and Guluronic Acid in Bacterial Alginate from Azotobacter vinelandii and the Effect on Glyceollin Induction and Accumulation in Soybeans

Ojokoh Eromosele

Asian Food Science Journal · pp. 80–91 · Published 17 Oct 2023

10.9734/afsj/2023/v22i10675

Abstract

Aims: The objective of this study was to investigate the eliciting efficacy of bacterial alginate which was extracted from Azotobacter vinelandii in the induction of glyceollins and accumulation in soybeans. Methodology: The preparative high performance liquid chromatography (HPLC) was used to detect, separate and purify the alginate after extraction and ultra performance liquid chromatography – mass spectrometry (UPLC-MS) was used to detect the molecular weights of both the extracted alginate and the induced glyceollins in soybeans. Results: Bacterial alginate induced glyceollins synthesis in soybean seeds in high quantity. The optimal conditions of elicitation were as follows: concentration of bacterial alginate 60 µL presoaked in the sterile water for 5h, incubated at a controlled temperature of 30℃ and in the dark for 4 days. Alginate induction ability exhibited glyceollin-inducing activities of 1.775 (mg/g dry weight) while the control had no record of glyceollin induction.  The results demonstrated that a larger molecular weight or a higher G/M ratio might correlate with a higher glyceollin-induction activity. Conclusion: In conclusion; alginate could be introduced as relatively safe and efficient elicitor of high glyceollin accumulation in soybeans. The method for bacterial alginate extraction and its application in soybean seeds for glyceollin induction may be of practical use in the food.

Glyceollins alginate elicitation accumulation and Azotobacter vinelandii

References (36)

  1. 1 REACTIVE OXYGEN SPECIES: Metabolism, Oxidative Stress, and Signal Transduction [DOI]
  2. 2 Plant defense in the absence of jasmonic acid: The role of cyclopentenones [DOI]
  3. 3 Alginates as a useful natural polymer for microencapsulation and therapeutic applications [DOI]
  4. 4 Monomer sequence and acetylation pattern in some bacterial alginates [DOI]
  5. 5 The influence of different biotic and abiotic elicitors on the production and profile of tropane alkaloids in hairy root cultures of Brugmansia candida [DOI]
  6. 6 Radiation-induced degradation of sodium alginate [DOI]
  7. 7 Soy Protein Reduces Serum Cholesterol by Both Intrinsic and Food Displacement Mechanisms [DOI]
  8. 8 Genetic and metabolic engineering of isoflavonoid biosynthesis [DOI]
  9. 9 Alginic acids in Lessonia vadosa: Partial hydrolysis and elicitor properties of the polymannuronic acid fraction [DOI]
  10. 10 Isolation and characterization of alginate-derived oligosaccharides with root growth-promoting activities [DOI]
  11. 11 Stimulation of human keratinocyte growth by alginate oligosaccharides, a possible co‐factor for epidermal growth factor in cell culture [DOI]
  12. 12 Antiestrogenic Glyceollins Suppress Human Breast and Ovarian Carcinoma Tumorigenesis [DOI]
  13. 13 Alginate oligosaccharides stimulate VEGF-mediated growth and migration of human endothelial cells [DOI]
  14. 14 Monomer composition and sequence of alginates from Pseudomonas aeruginosa [DOI]
  15. 15 Characterization of the Hydrolysis Mechanism of Polyalternating Alginate in Weak Acid and Assignment of the Resulting MG-Oligosaccharides by NMR Spectroscopy and ESI−Mass Spectrometry [DOI]
  16. 16 Sequence analysis of alginate-derived oligosaccharides by negative-ion electrospray tandem mass spectrometry [DOI]
  17. 17 Nitric oxide mediates alginate oligosaccharides-induced root development in wheat (Triticum aestivum L.) [DOI]
  18. 18 Effects of alginate oligosaccharides with different molecular weights and guluronic to mannuronic acid ratios on glyceollin induction and accumulation in soybeans [DOI]
  19. 19 Glyceollin improves endoplasmic reticulum stress-induced insulin resistance through CaMKK-AMPK pathway in L6 myotubes [DOI]
  20. 20 Alginate from Pseudomonas fluorescens and P. putida: production and properties [DOI]
  21. 21 Lactofen induces isoflavone accumulation and glyceollin elicitation competency in soybean [DOI]
  22. 22 Glyceollin-Elicited Soy Protein Consumption Induces Distinct Transcriptional Effects As Compared to Standard Soy Protein [DOI]
  23. 23 Purification of pectate oligosaccharides showing root-growth-promoting activity in lettuce using ultrafiltration and nanofiltration membranes [DOI]
  24. 24 Alleviation Effect of Alginate-Derived Oligosaccharides on Vicia faba Root Tip Cells Damaged by Cadmium [DOI]
  25. 25 Induction of phytochemical glyceollins accumulation in soybean following treatment with biotic elicitor (Aspergillus oryzae) [DOI]
  26. 26 Effects of biotic and abiotic elicitors on phytoalexin metabolism in soybean [DOI]
  27. 27 Alginate production by Azotobacter vinelandii in a membrane bioreactor [DOI]
  28. 28 Synergistic effect of active oxygen species and alginate on chitinase production by Wasabia japonica cells and its application [DOI]
  29. 29 Detection of Alginate Oligosaccharides from Mollusks [DOI]
  30. 30 Analysis of carbohydrates using different quaternized polystyrene-divinylbenzene particles and pulsed amperometric detection [DOI]
  31. 31 Effects of Alginate Oligosaccharides on the Accumulation of Glyceollins in Soybean
  32. 32 Regulation by sulfhydryl groups of glyceollin accumulation in soybean hypocotyls [DOI]
  33. 33 Bacterially-induced glyceollin production in soybean cell suspension cultures [DOI]
  34. 34 Experimental strategy to assess the main engineering parameters characterizing sodium alginate recovery from model solutions by ceramic tubular ultrafiltration membrane modules [DOI]
  35. 35 Journal of Food Science and Technology [DOI]
  36. 36 Journal of the science of food and agriculture [DOI]

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