Skip to content
Research Article Open access CC BY 4.0

Identification of Immunogenic T and B-Cell Epitope Peptides of Rubella Virus E1 Glycoprotein towards the Development of Highly Specific Immunoassays and Vaccine

Prashanth Rajendiran, Nithiyanandan Saravanan, Mageshbabu Ramamurthy, Rajasekar Aruliah, Balaji Nandagopal

Journal of Advances in Biology & Biotechnology · pp. 37–43 · Published 18 Oct 2022

10.9734/jabb/2022/v25i7588

Abstract

Introduction: The Rubella virus has a worldwide occurrence and congenital Rubella syndromes are widely recognized as an emerging infection in several parts of the world. Miscarriage, perinatal mortality, and stillbirth can develop in pregnancy during the first trimester. The most frequent techniques for laboratory diagnosis of Rubella virus infection are IgM and IgG-based serological detection methods. Such emerging viral and bacterial pathogen emphasizes the development of fast diagnostic devices; there is a need for enhanced and quicker methods. Materials and Methods: Search for peptide vaccine with specific T and B-cell epitopes was identified through bioinformatics-based approaches. These were identified utilizing available Rubella virus E1 glycoprotein sequence databases. The outer-membrane glycoprotein, E1 is a target protein for the prediction of best antigens. Results: Using BepiPred2 program, the potential B-cell epitope PFCNTPHGQLEVQVPPDPGD    with high conservation among E1 glycoprotein of rubella virus and the maximum surface exposed residues was identified.  Using IEDB, NetMHCpan, and NetCTL programs, T-cell epitope RPVALPRAL was identified. Predicted epitopes were found to have promiscuous class-I major histocompatibility complex binding affinity to major histocompatibility complex super types, antigenicity scores, and high proteasomal cleavage. The three-dimensional modeled structures were created using I-TASSER online server for highlighting the predicted T- and B- cell epitopes. Conclusion: The predicted T and B cell epitope could be used for the development of immunoglobulin assay and vaccine candidate peptide.

Epitope Rubella virus peptide vaccine B and T cell peptide

References (18)

  1. 1 The I-TASSER Suite: protein structure and function prediction [DOI]
  2. 2 Epidemiology and Prevention of Vaccine-Preventable Diseases
  3. 3 BepiPred-2.0: improving sequence-based B-cell epitope prediction using conformational epitopes [DOI]
  4. 4 Gapped sequence alignment using artificial neural networks: application to the MHC class I system [DOI]
  5. 5 Reliable B Cell Epitope Predictions: Impacts of Method Development and Improved Benchmarking [DOI]
  6. 6 Classification of Nuclear Receptors Based on Amino Acid Composition and Dipeptide Composition [DOI]
  7. 7 Adverse Events Following Measles, Mumps, and Rubella Vaccine in Adults Reported to the Vaccine Adverse Event Reporting System (VAERS), 2003–2013 [DOI]
  8. 8 Molecular aspects of the teratogenesis of rubella virus [DOI]
  9. 9 Cryo-Electron Tomography of Rubella Virus [DOI]
  10. 10 Sequence of the genome RNA of rubella virus: Evidence for genetic rearrangement during togavirus evolution [DOI]
  11. 11 Anaphylactic reactions to measles–mumps–rubella vaccine in three children with allergies to hen’s egg and cow’s milk [DOI]
  12. 12 Sero-prevalence of rubella among pregnant women in India, 2017 [DOI]
  13. 13 The key role of rubella virus glycoproteins in the formation of immune response, and perspectives on their use in the development of new recombinant vaccines [DOI]
  14. 14 Identification of rubella virus T-cell epitopes recognized in anamnestic response to RA27/3 vaccine: associations with boost in neutralizing antibody titer [DOI]
  15. 15 Expression and characterization of a soluble rubella virus E1 envelope protein [DOI]
  16. 16 A synthetic peptide ELISA for the screening of rubella virus neutralizing antibodies in order to ascertain immunity [DOI]
  17. 17 Centers for Disease Control and Prevention [DOI]
  18. 18 Genomic scans for selective sweeps using SNP data [DOI]

Cited by 0

No indexed citations yet.

Related research

Article metrics

Real usage data collected on this platform.

17

Page views

0

PDF downloads

7

Outbound clicks

0

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.