Identification of Mixed Virus Infection on Trichosanthes cucumerina L. in Akamkpa,  Southern Cross River State, Nigeria

O. I. Eyong; E. E. Ekpiken; D. E. Akam; A. T. Owolabi

doi:10.9734/ajaar/2021/v15i230150

Research Article Open access CC BY 4.0

Identification of Mixed Virus Infection on Trichosanthes cucumerina L. in Akamkpa, Southern Cross River State, Nigeria

O. I. Eyong, E. E. Ekpiken, D. E. Akam, A. T. Owolabi

Asian Journal of Advances in Agricultural Research · pp. 53–58 · Published 19 May 2021

10.9734/ajaar/2021/v15i230150

Abstract

Trichosanthes cucumerina L is a tropical or subtropical fruit bearing crop of the Cucurbitaceae family. Field survey during 2020 planting season in Akamkpa, Southern Cross River State, Nigeria revealed widespread virus infection of the crop. Symptoms observed included severe leaf malformation/reduction and rugosity. This research was therefore aimed at identifying viruses infecting Trichosanthes cucumerina in this location. Infected leaf samples were collected and virus maintained on young seedlings of T. cucumerina in the Botanical Garden of University of Calabar, Nigeria. Diagnostic tools included ACP-ELISA and gene sequence analysis. The ELISA result revealed two viruses belonging to the genus Cucumovirus and Potyvirus. The identity of the viruses were determined after virus sequences were compared with other viruses available in the GenBank of National Centre for Biotechnology Information (NCBI) using the basic local alignment search tool (BLASTn). The result revealed the Cucumovirus to be Cucumber mosaic virus (CMV) while the Potyvirus was revealed to be Potato virus Y (PVY). This is the first report of mixed virus infection on Trichosanthes cucumerina in Nigeria.

Cucurbitaceae ELISA cucumovirus amplicon potyvirus

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O. I. Eyong, E. E. Ekpiken, D. E. Akam, A. T. Owolabi (2021). Identification of Mixed Virus Infection on Trichosanthes cucumerina L. in Akamkpa, Southern Cross River State, Nigeria. Asian Journal of Advances in Agricultural Research , 53–58. https://doi.org/10.9734/ajaar/2021/v15i230150

Affiliations

O. I. Eyong — Department of Forestry and Wildlife Management, Cross River University of Technology, Nigeria.
E. E. Ekpiken — Department of Plant Science and Biotechnology, Cross River University of Technology, Nigeria.
D. E. Akam — Department of Plant Science and Biotechnology, Cross River University of Technology, Nigeria.
A. T. Owolabi — Department of Plant and Ecological Studies, University of Calabar, Calabar, Nigeria.

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