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Research Article Open access CC BY 4.0

Ileum Histoarchitecture Following Aspartame Consumption: Assessment of Actions of Moringa oleifera Seed Extract

Christopher Chiedozie Mbadugha, Donatus Gabriel Edem, Iboro Efiong Edet, Iberi Patrick Amagwu, Francis Nkem Odinukaeze, Abasikponke Ralphael Godwin

Journal of Advances in Medical and Pharmaceutical Sciences · pp. 1–10 · Published 1 Aug 2022

10.9734/jamps/2022/v24i730311

Abstract

This study aims to determine the effect of Moringa oleifera seed extract on ileum histoarchitecture following aspartame consumption. Twenty-seven adult male Albino Wistar rats were used for the study. They were divided into nine groups comprising of: group A (control), administered distilled water; group B, given 0.4 ml (low dose) aspartame; group C, given 1.5 ml (high dose) aspartame; group D1, given 0.6 ml (low dose) aspartame for the first three weeks followed by 1.5 ml (low dose) ethanolic extract of M. oleifera seed on the fourth week; group D2 given 0.5 ml (low dose) aspartame for the first three weeks followed by 1.30 ml (low dose) n-hexane extract of M. oleifera seed on the fourth week; group E1, given 1.5 ml (high dose) aspartame for the first three weeks and on the fourth week 2.5 ml (high dose) ethanolic extract of M. oleifera seed; group E2, given 1.5(high dose) aspartame for the first three weeks and on the fourth week 3.0 ml (high dose) n-hexane extract of M. oleifera seed; group F1 given 1.4ml (high dose) aspartame followed by 2.8ml (high dose) ethanolic extract of M. oleifera seed for four weeks; group F2, given 1.3 ml (high dose) aspartame followed by 2.5 ml (high dose) n-hexane extract of M. oleifera seed for four weeks. Weight measurement of rats was taken once a week throughout the twenty eight days duration of the study. Following anaesthetization, their ileum were harvested and fixed in 10 % buffered formalin. Hematoxylin and Eosin (H&E) staining technique was adopted for routine histological procedure. Data were expressed as mean ± standard error of mean (M ± SEM) and subjected to one-way analysis of variance (ANOVA). At 95 % confidence interval, P is significant (P< 0.05). There was a significant increase (P= 0.05) in body weight of both the control and the experimental groups as the experiment progressed. Histological findings revealed that aspartame both in low and high doses has disruptive effects on the histoarchitecture of ileum and high dose ethanolic extract of M. oleiferia seed has the capacity to ameliorate the effects. Hence, caution is needed in aspartame consumption.

Albino wistar rats aspartame ethanolic extract Moringa oleifera seed N–hexane extract Moringa oleifera seed

References (16)

  1. 1 Artificial sweeteners induce glucose intolerance by altering the gut microbiota [DOI]
  2. 2 Clinically Oriented Anatomy
  3. 3 Phytochemical screening of Nigerian medicinal plants II.
  4. 4 Lipopolysaccharide Regulation of Intestinal Tight Junction Permeability Is Mediated by TLR4 Signal Transduction Pathway Activation of FAK and MyD88 [DOI]
  5. 5 Nutritional potential of two leafy vegetables: Moringa oleifera and Ipomoea batatas leaves [DOI]
  6. 6 diFiore's Atlas of Histology with Functional Correlations
  7. 7 Reshaping the gut microbiota: Impact of low calorie sweeteners and the link to insulin resistance? [DOI]
  8. 8 A review of the effectiveness of aspartame in helping with weight control [DOI]
  9. 9 Artificial Sweeteners Disrupt Tight Junctions and Barrier Function in the Intestinal Epithelium through Activation of the Sweet Taste Receptor, T1R3 [DOI]
  10. 10 Gain weight by "going diet?"Artificial sweeteners and the neurobiology of sugar cravings
  11. 11 Long-Term Aspartame Administration Leads to Fibrosis, Inflammasome Activation, and Gluconeogenesis Impairment in the Liver of Mice [DOI]
  12. 12 Effects of artificial sweeteners on body weight, food and drink intake [DOI]
  13. 13 An evaluation of the effect of aspartame on weight loss [DOI]
  14. 14 Low-energy substitutes for sugars and fats in the human diet: Impact on nutritional regulation [DOI]
  15. 15 Acute Impact of the Artificial Sweetener Aspartame on the Ultrastructures of Hepatocyte in Mice [DOI]
  16. 16 Moringa oleifera Seed Actions Following Aspartame Consumption: A Systematic Evaluation of the Large Intestine [DOI]

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