Immature Embryo-Derived of Two Bread Wheat (Triticum aestivum L.) Varieties Transformation Using Particle Bombardment Method
D. C. Tinak Ekom, S. M. Udupa, M. N. Benchekroun, M. M. Ennaji, R. Abdelwahd, D. Iraqi
Annual Research & Review in Biology · pp. 3904–3914 · Published 12 Jul 2014
10.9734/ARRB/2014/8499Abstract
Aims: Wheat cultivation is still difficult to control because it faces several biotic and abiotic stresses. The transfer of resistance in wheat plants to these stresses through traditional approaches remains limited. However, genetic transformation allows the acquisition of this tolerance while overcoming the difficulties of classical improvement. Methodology: In this context, immature embryos of two Moroccan bread wheat varieties (‘Massira’ and ‘Arréhane’) and the pBY520 plasmid containing the HVA1 gene for drought tolerance as well as the gene bar selection marker for resistance to the herbicide phosphinothricin were used in this study for bombardment protocol. Results: The percentage of calli bombarded with regenerated plantlets was 23.92 % for the variety ‘Massira’ and 5.26% for the variety ‘Arréhane’. The selection was carried out on ½ MS rooting medium lacking hormones and supplemented with phosphinothricin (3 mg l-1); 4.36% and 6% of plantlets of varieties ‘Massira’ and ‘Arréhane’ respectively survived. The resistant plantlets were transferred to the greenhouse and the evaluation of the expression of the bar gene in leaves was positive about 60%. The confirmation by molecular analysis revealed only a transformation efficiency of 0.52 % for the variety ‘Arréhane’.
References (33)
- 1 Herbicide Resistant Fertile Transgenic Wheat Plants Obtained by Microprojectile Bombardment of Regenerable Embryogenic Callus [DOI]
- 2 Stress-induced synthesis of proline confers tolerance to water deficit in transgenic wheat [DOI]
- 3 HVA1, a LEA gene from barley confers dehydration tolerance in transgenic rice (Oryza sativa L.) via cell membrane protection [DOI]
- 4 Genetic engineering of wheat for increased resistance to powdery mildew disease [DOI]
- 5 A rice stress-responsive NAC gene enhances tolerance of transgenic wheat to drought and salt stresses [DOI]
- 6 Identification of highly transformable wheat genotypes for mass production of fertile transgenic plants [DOI]
- 7 Rapid Production of Multiple Independent Lines of Fertile Transgenic Wheat (Triticum aestivum) [DOI]
- 8 Self‐fertile transgenic wheat plants regenerated from isolated scutellar tissues following microprojectile bombardment with two distinct gene constructs† [DOI]
- 9 Wheat transformation: current technology and applications to grain development and composition [DOI]
- 10 Agrobacterium-mediated large-scale transformation of wheat (Triticum aestivum L.) using glyphosate selection [DOI]
- 11 Glyphosate-tolerant CP4 and GOX genes as a selectable marker in wheat transformation [DOI]
- 12 Genotyping with RAPD and microsatellite markers resolves pathotype diversity in the ascochyta blight pathogen of chickpea [DOI]
- 13 The development of a nuclear male sterility system in wheat. Expression of the barnase gene under the control of tapetum specific promoters [DOI]
- 14 Analysis of particle bombardment parameters to optimise DNA delivery into wheat tissues [DOI]
- 15 Procedures allowing the transformation of a range of European elite wheat (Triticum aestivum L.) varieties via particle bombardment [DOI]
- 16 Genetic improvement of wheat via alien gene transfer, an assessment [DOI]
- 17 Phosphomannose isomerase, pmi, as a selectable marker gene for durum wheat transformation [DOI]
- 18 Ammonium nitrate improves direct somatic embryogenesis and biolistic transformation of Triticum aestivum [DOI]
- 19 Rapid and efficient cosmid cloning [DOI]
- 20 Age‐dependent transformation frequency in elite wheat varieties [DOI]
- 21 Green fluorescent protein as a visual marker for wheat transformation [DOI]
- 22 Callus induction and plant regeneration from immature and mature embryos of winter durum wheat genotypes [DOI]
- 23 Comparison of three selectable marker genes for transformation of wheat by microprojectile bombardment [DOI]
- 24 Induced Expression of DREB Transcriptional Factor and Study on Its Physiological Effects of Drought Tolerance in Transgenic Wheat [DOI]
- 25 Genotype-dependent Stable Genetic Transformation in German Spring Wheat Varieties Selected for High Regeneration Potential [DOI]
- 26 Establishment of a Highly Efficient Regeneration System for the Mature Embryo Culture of Wheat
- 27 The Selection of Transgenic Recipients from New Elite Wheat Cultivars and Study on Its Plant Regeneration System
- 28 Wheat Transformation Using Cyanamide as a New Selective Agent [DOI]
- 29 Genetic transformation of wheat using mature seed tissues [DOI]
- 30 Genetic transformation of mature embryos of bread ( T. aestivum ) and pasta ( T. durum ) wheat genotypes [DOI]
- 31 Mature embryo-derived wheat transformation with major stress-modulated antioxidant target gene [DOI]
- 32 Marker Gene Delivery to Mature Wheat Embryos Via Particle Bombardment
- 33 Study on the Factors Influencing the Efficiency of Wheat Transformation
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