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Research Article Open access CC BY 4.0

Immunogenicity Studies of Various Experimental Vaccines in Chickens

Angel Justiz Vaillant, Patrick E. Akpaka

Biotechnology Journal International · pp. 41–55 · Published 9 Jun 2023

10.9734/bji/2023/v27i3685

Abstract

In this paper, the main objective was to raise chickens’ antibodies against three crucial public health microorganisms: the human immunodeficiency virus-1, Salmonella spp, and Staphylococcus aureus. Immunogens were prepared from the said microorganisms. Chickens were vaccinated either orally or intramuscularly. After a booster immunization, mostly eggs were collected and assess for the presence of specific antibodies. The most important results were the production of a large amount of anti-HIV antibodies in chicken’s eggs, and also the synthesis of anti-protein a antibodies with the ability to inhibit the growth of S. aureus in vitro and to serve as anti-anti-idiotypic antibodies with the capacity of neutralizing the original antigen. Enzyme- linked immune absorbent assays detected the presence of these antibodies as anti-Salmonella antibodies that were critical in reducing the bacterial load in the stomach and caeca compared with a control group. The vaccines were effective and safe, but more laboratory work, and economics have to be carried out to start a human trial.

Microbiology vaccinology anti-idiotypic antibodies chickens protein purification

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Cited by 2

Tackling Infectious Diseases in the Caribbean and South America: Epidemiological Insights, Antibiotic Resistance, Associated Infectious Diseases in Immunological Disorders, Global Infection Response, and Experimental Anti-Idiotypic Vaccine Candidates Against Microorganisms of Public Health Importance

Angel Justiz-Vaillant, Sachin Soodeen, Darren Gopaul · Microorganisms · 2025

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