Impact of Acidic Beverages on Colour Stability of Enamel Using Different Dentrifice: A Pilot Study

Abstract

Background: The frequent consumption of beverages containing high concentrations of organic pigments, such as tea, coffee, acai, wine, and cola-based soft drinks, can lead to tooth staining. Due to the increased demand for patients seeking white and stain-free teeth, the dental products industry has launched mouthwashes and dentifrices that claim to whiten teeth. Aim: The study aimed to evaluate the effect of whitening dentifrice containing BC on the colour of enamel stained with different beverages. Methodology: This is an experimental in vitro study, comparative, with parallel groups, controlled, and randomised. Fifteen molars were sectioned to obtain enamel specimens, which were sectioned and divided into 6 groups: distilled water, cola-based soft drink, wine, acai, coffee, and dark beer. The colour of the specimens was evaluated using a spectrophotometer. Subsequently, they were immersed in the beverages for 6 hours/day, for a total of 30 days. Each specimen was inserted into a brushing machine containing a solution with BC and immersed in 5 mL of a solution containing BC-based whitening dentifrice (Close Up White Now Glacier Fresh; Unilever, São Paulo, SP, Brazil), artificial saliva, and distilled water, obtained by diluting equal volumes of each component (1:1:1). All data were analysed by ANOVA and the Student-Newman-Keuls test (α=0.05). Results: As for Δb*, no statistical difference was observed among the distilled water, cola-based soft drink, wine, and coffee groups (p>0.05). Also, no statistical difference was found between the acai and dark beer groups (p>0.05). Splenic diameter ratio (1676.7 to 824.6) declined significantly. Logistic regression showed splenic collaterals and platelets are significantly but negatively associated with oesophageal varices grades. Conclusion: The whitening dentifrice containing BC was effective in altering the colour of tooth enamel stained with coffee, the cola-based soft drink, wine, acai, and dark beer.