Improved Decipherment of the Protein Database of Human Proteins in the PDMD (Protein-Direct-Microsequencing-Deciphering) Method
Annual Research & Review in Biology · pp. 47–49 · Published 25 Apr 2022
10.9734/arrb/2022/v37i430501Abstract
Human proteins seem to be processed by Human serum biotinidase, and Human excreted proteins seem to be handled with by Human serum biotinidase and Human Chymotrypsin A. Therefore, we must improve PDMD method by using these new findings. Protein determination is performed by the highly sensitive HPLC-SEC-photometric method at UV 210 nm; i.e., c.a. 200-fold sensitive than Lowry’s method. Human proteins are found to be not metabolized at membrane inserted portions. Membrane and Hydrophobic proteins of Humans are defined as the precipitable proteins at 100,000 x g for 90 min at 4 C, and have hydrophobicities larger than 0.515.
References (8)
- 1 Size-exclusion chromatography of biological samples which contain extremely alkaline proteins [DOI]
- 2 Urinary Biotinidase and Alanine Excretion in Patients with Insulin-Dependent Diabetes Mellitus [DOI]
- 3 Lipoamidase activity in normal and mutagenized pancreatic cholesterol esterase (bile salt-stimulated lipase) [DOI]
- 4 Protein determination by high-performance gel-permeation chromatography: applications to human pancreatic juice, human bile and tissue homogenate [DOI]
- 5 Structural changes in the activation of chymotrypsinogen and trypsinogen. Effect of urea on chymotrypsinogen and delta-chymotrypsin [DOI]
- 6 Comparative study of human milk and serum biotinidase [DOI]
- 7 Enkephalin hydrolysis by human serum biotinidase [DOI]
- 8 Improved recovery of ovalbumin by reversed-phase high-performance liquid chromatography [DOI]
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