In-vitro Propagation of Aloe vera (L.) Burm. f
Sharmistha Gupta, Pankaj K. Sahu, Devki L. Sen, Priyanka Pandey
Biotechnology Journal International · pp. 806–816 · Published 12 Jul 2014
10.9734/BBJ/2014/9747Abstract
Department of Botany, Dr. C.V. Raman University, Bilaspur, (C.G.) India Aim: In vitro culture is used for commercial production and is achieved in aseptic condition using different concentration and combination of Plant Growth Regulators (PGR). Material and Methods: In present work studied the effects of different plant growth regulator singly or in combination in tissue culture of Aloe vera (L.) Burm. f. belongs to the family Liliaceae or Asphodelaceae, used in ayurveda as well as pharmaceutical industry. Murashige and Skoog (MS) media with different combinations and concentration of growth promoters i.e. Auxin (Indole-3-butyric acid (IBA), α-Naphthalene acetic acid (NAA) and Indole-3-acetic acid (IAA) and cytokinin (Benzyl Amino Purine (BAP) were used in this study. Surface sterilization was standardised with HgCl2 with various concentration and time. Observation and Result: The development of callus type was observed in the MS media supplemented with BAP for best regeneration, IBA for root formation and NAA, was found the best media for root formation (0.5mg/l) were seen to grow onwards from the tenth day of culture and 90% of root formation took place within a span of 3-4 weeks for maximum callus induction. The maximum number of root & shoot produced is 4.8±0.53 with average length of 3.5±0.35cm. Discussion: The present work deals with in vitro plant growth of Aloe vera through tissue culture for propagation and ex situ conservation. Regenerated plants after acclimatization were transferred to soil and they showed 85% survival. The culture response was maximum in apical buds (100%) and minimum time required is 9.67 days in the same media. The average length of shoots per culture was 4.0±0.16cm. In present study among the three types of auxins, NAA was found to be the best for root induction.
References (16)
- 1 A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures [DOI]
- 2 Systematic Review of Herbs and Dietary Supplements for Glycemic Control in Diabetes [DOI]
- 3 A review of the genetic effects of naturally occurring flavonoids, anthraquinones and related compounds [DOI]
- 4 Therapeutic and Medicinal Uses of Aloe vera: A Review [DOI]
- 5 Micropropagation of the endangered Aloe polyphylla [DOI]
- 6 Tissue Culture Propagation of Elite Plant of Aloe vera Linn [DOI]
- 7 Rapid in vitro propagation of Aloe barbadensis Mill [DOI]
- 8 Micropropagation of Aloe vera L. Grown in South Iran [DOI]
- 9 Studies on stem tissue culture and organogenesis of Aloe vera.
- 10 Whole plant regeneration of Cicer arietinum from callus cultures via organogenesis [DOI]
- 11 Establishment of Aloe, Gasteria, and Haworthia Shoot Cultures from Inflorescence Explants [DOI]
- 12 In vitro propagation of monocotyledonous bulbs and corms
- 13 A preliminary study on the induction and propagation of adventitious buds for Aloe vera L.
- 14 Introduction of the Most Suitable Culture Media for Micropropagation of a Medicinal Plant Aloe (Aloe barbadensis Mill.)
- 15 STUDY ON PROPAGATION IN VITRO OF ALOE
- 16 Plant Tissue Culture: Theory and Practice
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