In vitro Screening of Natural Metarhizium spp. Isolates for Bioinsecticidal and Biofungicidal Activity
Tinatin Doolotkeldieva, Sagynych Tolonbekova, Saykal Bobusheva
Journal of Advances in Microbiology · pp. 103–121 · Published 28 Feb 2024
10.9734/jamb/2024/v24i2799Abstract
Aims: To increase conidia production of local Metarhizium spp—strains by selecting the low-cost media to test obtained bioformulation against the sucking pests and fungal plant pathogens. Study Design: The phytophages like black bean aphid (Aphis fabae), a scale insect (Aspidiotus nerii) and spider mite (Tetranychus urticae) are economically essential pests in Kyrgyzstan. The current requirement is to use environmentally friendly protection to reduce the number of these pests. The entomopathogenic fungus from the Metarhizium genus was used to reduce harmful pests in vitro and in vivo experiments. Place and Duration of Study: Department of Plant Protection, Kyrgyz-Turkish Manas University between May 2021 and May 2023. Methodology: Natural Metarhizium spp. strains were isolated from dead insect bodies of the Lepidoptera and Apidae families. A laboratory bioassay was conducted to evaluate isolated Metarhizium spp.strains against nymphal stages and adults of sucking pests like A. fabae ((Blackfly), A.nerii (Scale insect) and T.urticae (Spider mite). The inhibitory effect of Metarhizium spp.against Fusarium oxysporum, Alternaria spp. and Rhizoctonia solani was detected using a dual culture technique. Results: The results of in vitro bioassay tests against sucking pests have revealed the LC50 and LT50 values of TLK-1 isolate. The LC50 of this isolate for A. fabae adults was 1 × 106 conidia\ mL-1; for A.nerii, it was lower - 7 × 105 conidia\ ml-1; while for adults T.urticae it was higher- 13 × 106 conidia \ ml-1. Such mortality occurred after 49.63 hours in adults of A. fabae (LT50), after 46.30 hours in A.nerii (LT50), and after 75.87 hours in deutonymph and adults of T. urticae (LT50). Three isolates, TLK-1, TLA-2 and BZТК1, have exhibited biofingicide activity in pathogen fungi like Fusarium oxysporum and Alternaria spp. Conclusion: The found isolates can be simultaneously used against pathogenic fungi, essential for developing inexpensive biological formulas based on these fungi.
References (51)
- 1 MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0 [DOI]
- 2 AMPLIFICATION AND DIRECT SEQUENCING OF FUNGAL RIBOSOMAL RNA GENES FOR PHYLOGENETICS [DOI]
- 3 Mycoinsecticides and Mycoacaricides: A comprehensive list with worldwide coverage and international classification of formulation types [DOI]
- 4 Fungal entomopathogens: new insights on their ecology [DOI]
- 5 Genome Sequencing and Comparative Transcriptomics of the Model Entomopathogenic Fungi Metarhizium anisopliae and M. acridum [DOI]
- 6 The production and uses of Beauveria bassiana as a microbial insecticide [DOI]
- 7 Metarhizium spp., Cosmopolitan Insect-Pathogenic Fungi: Mycological Aspects [DOI]
- 8 Endophytic Insect-Parasitic Fungi Translocate Nitrogen Directly from Insects to Plants [DOI]
- 9 Colony interactions and hyphal interference between Septoria nodorum and phylloplane fungi [DOI]
- 10 A taxonomic revision of Metarhizium based on a phylogenetic analysis of rDNA sequence data [DOI]
- 11 International Research Journal of Biological Sciences
- 12 The insect‐pathogenic fungus Metarhizium robertsii (Clavicipitaceae) is also an endophyte that stimulates plant root development [DOI]
- 13 Experimental and Applied Acarology [DOI]
- 14 Prospects of Botanical Biopesticides in Insect Pest Management [DOI]
- 15 Beauveria bassiana and Metarhizium anisopliae endophytically colonize cassava roots following soil drench inoculation [DOI]
- 16 Microbial biopesticides for invertebrate pests and their markets in the United States [DOI]
- 17 The plant beneficial effects of Metarhizium species correlate with their association with roots [DOI]
- 18 Antioxidant enzymes and their contributions to biological control potential of fungal insect pathogens [DOI]
- 19 Endophytic Metarhizium robertsii promotes maize growth, suppresses insect growth, and alters plant defense gene expression [DOI]
- 20 Algal-fungal symbiosis leads to photosynthetic mycelium [DOI]
- 21 The genome sequence of the biocontrol fungus Metarhizium anisopliae and comparative genomics of Metarhizium species [DOI]
- 22 The multifunctional lifestyles of Metarhizium: evolution and applications [DOI]
- 23 Effect of endophytic entomopathogenic fungi on soybean Glycine max (L.) Merr. growth and yield [DOI]
- 24 Insights into regulatory roles of MAPK-cascaded pathways in multiple stress responses and life cycles of insect and nematode mycopathogens [DOI]
- 25 Effects of Endophytic Entomopathogenic Ascomycetes on the Life-History Traits of Aphis gossypii Glover and Its Interactions with Melon Plants [DOI]
- 26 Selection of Entomopathogenic Fungi for Aphid Control [DOI]
- 27 Characterization of Metarhizium species and varieties based on molecular analysis, heat tolerance and cold activity [DOI]
- 28 Influence of Moisture Content on Temperature Tolerance and Storage of Metarhizium flavoviride Conidia in an Oil Formulation [DOI]
- 29 Fungal entomopathogens as endophytes reduce several species of Fusarium causing crown and root rot in sweet pepper (Capsicum annuum L.) [DOI]
- 30 Species limits, phylogeography and reproductive mode in the Metarhizium anisopliae complex [DOI]
- 31 Assessment of entomopathogenic fungi and their extracts against a soil-dwelling pest and soil-borne pathogens of olive [DOI]
- 32 Native entomopathogenic Metarhizium spp. from Burkina Faso and their virulence against the malaria vector Anopheles coluzzii and non-target insects [DOI]
- 33 Effect of Metarhizium anisopliae inoculation on the mating behavior of three species of African Tephritid fruit flies, Ceratitis capitata, Ceratitis cosyra and Ceratitis fasciventris [DOI]
- 34 Evaluation of virulence, tolerance to environmental factors and antimicrobial activities of entomopathogenic fungi against two-spotted spider mite, Tetranychus urticae [DOI]
- 35 Transmission potential of the entomopathogenic fungi Isaria fumosorosea and Beauveria bassiana from sporulated cadavers of Diaphorina citri and Toxoptera citricida to uninfected D. citri adults [DOI]
- 36 Influence of two formulation types and moisture levels on the storage stability and insecticidal activity of Beauveria bassiana [DOI]
- 37 Systemic Colonization by Metarhizium robertsii Enhances Cover Crop Growth [DOI]
- 38 Entomopathogen Metarhizium anisopliae promotes the early development of peanut root [DOI]
- 39 Biological control of papaya aphid (Aphis gossypii Glover) using entomopathogenic fungi [DOI]
- 40 Susceptibility of developmental stages of Tetranychus urticae (Acari: Tetranychidae) to infection by Beauveria bassiana and Metarhizium anisopliae (Hypocreales: Clavicipitaceae) [DOI]
- 41 Can Potato Plants Be Colonized with the Fungi Metarhizium and Beauveria under Their Natural Load in Agrosystems? [DOI]
- 42 Virulence and transgenerational effects of Metarhizium anisopliae on Oxycarenus hyalinipennis [DOI]
- 43 Prospects for biopesticides for aphid control [DOI]
- 44 Beauveria Bassiana Pathogenicity to the Citrus Rust Mite Phyllocoptruta Oleivora [DOI]
- 45 External events related to the infection process of Cornitermes cumulans (Kollar) (Isoptera: Termitidae) by the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae [DOI]
- 46 Metarhizium aciculare sp. nov. for euvesperins A and B producing Metarhizium strains [DOI]
- 47 Screening of Fungal Strains and Formulations of Metarhizium anisopliae to Control Phyllotreta striolata in Chinese Flowering Cabbage [DOI]
- 48 Observations on the Relationships between Endophytic Metarhizium robertsii, Spodoptera frugiperda (Lepidoptera: Noctuidae), and Maize [DOI]
- 49 Virulence of Some Entomopathogenic Fungi Isolates of Beauveria bassiana (Hypocreales: Cordycipitaceae) and Metarhizium anisopliae (Hypocreales: Clavicipitaceae) to Aulacaspis tubercularis (Hemiptera: Diaspididae)and Icerya seychellarum (Hemiptera: Monophlebidae) on Mango Crop [DOI]
- 50 Biomass Production of Entomopathogenic Fungi using various Agro Products in Kota Region, India
- 51 Species Discrimination within the Metarhizium PARB Clade: Ribosomal Intergenic Spacer (rIGS)-Based Diagnostic PCR and Single Marker Taxonomy [DOI]
Cited by 0
No indexed citations yet.
Related research
- Yield of Metarhizium anisopliae Conidia on Four Tropical Cereals and in vitro Evaluation of Virulence against Cowpea Weevil, Callosobruchus maculatus (Fabricius) (Coleoptera: Chrisomelidae) — shares topic coverage
- Natural Infestation of Coelaenomenodera lameensis (Coleoptera: Chrysomelidae-Hispinae) by Entomopathogenic Fungi in Oil Palm Groves in the Southern Part of Côte d’Ivoire — shares topic coverage
- Seasonal Incidence and Eco Friendly Management of White Grub in Sugarcane Using Entomopathogenic Nematode and Entomopathogenic Fungus in Andhra Pradesh, India — shares topic coverage
- Determination of Optimal Culture Medium Conditions for Mass Production of Beauveria bassiana Clone EF 46 — shares topic coverage
- Isolation and Characterization of Indigenous Isolates of the Entomopathogenic Fungus Aschersonia placenta Berk. — shares topic coverage
Article metrics
Real usage data collected on this platform.
0
Page views
0
PDF downloads
0
Outbound clicks
0
Citations
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
No views recorded yet.
Traffic sources
Referring site, by host.
No traffic recorded yet.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.