Skip to content
Research Article Open access CC BY 4.0

mir186 and mir145 In vivo Evaluation and Enrichment in Rats Submitted to Treadmill Strenuous Exercise

Raquel Martins De Freitas, Christina Pacheco, Stela Mirla Da Silva Felipe, Jannison Karlly Ribeiro Cavalcante, Paulo Elesson Oliveira, Denner Silvino Da Silva, Vânia Marilande Ceccatto

International Journal of Biochemistry Research & Review · pp. 31–44 · Published 25 Oct 2021

10.9734/ijbcrr/2021/v30i630274

Abstract

Aims: The present study aimed to identify miRNAs differentially expressed in rats submitted to strenuous exercise and in silico investigation of the biological implication of the findings. Place and Duration of Study: The in vivo experiments and analyses were performed in the Laboratory of Biochemistry and Gene Expression – LABIEX of the Superior Institute of Biomedical Science – ISCB from the State University of Ceará. Between 2017-2020. Methodology: The study was performed using as subjects 2-month-old male wistar rats, which initially were submitted to 2-week adaptation training. Later the animals were separated in two distinct groups, control (C) and trained (T), where only T performed a single session of strenuous exercise, while C were not submitted to this treatment.  The applied exercise protocol consisted in a running training in treadmill with speed constant increasing until the animal exhaustion which was measured by the animal refusal to keep running. After 24h, soleus muscle was desiccated and submitted to RNAseq sequencing protocols. Obtained data were statistically evaluated in R environment with EBSeq package, to characterize and predict the miRNAs and their targets were used bioinformatics tools Gene Cards, mi RBase enrichR and KEGG. Results: Two differentially expressed miRNAs were found, mir145 and mir 186, both with downregulated expression pattern in strenuous exercise. These miRNAs have a total of 1201 predicted target genes, 67 were repeated and mostly correlate to cardiovascular disease pathways, between those 5 were differentially expressed as down-regulated. Conclusion: In conclusion, the findings suggest that mir186 and mir145 down-regulation profile mediated by strenuous exercise implicates in the non-alteration of the target genes expression profile, and consequently did not mediate alterations in the pathways they are evolved, which are mainly related to signaling and disorders.

mir145 and mir186 RNAseq strenuous exercise data mining

References (47)

  1. 1 Enrichr: a comprehensive gene set enrichment analysis web server 2016 update [DOI]
  2. 2 The GeneCards Suite: From Gene Data Mining to Disease Genome Sequence Analyses [DOI]
  3. 3 Overview of MicroRNA Biogenesis, Mechanisms of Actions, and Circulation [DOI]
  4. 4 miRBase: from microRNA sequences to function [DOI]
  5. 5 KEGG for integration and interpretation of large-scale molecular data sets [DOI]
  6. 6 The Sequence Read Archive [DOI]
  7. 7 InteractiVenn: a web-based tool for the analysis of sets through Venn diagrams [DOI]
  8. 8 miR-145 and miR-143 regulate smooth muscle cell fate and plasticity [DOI]
  9. 9 EBSeq: an empirical Bayes hierarchical model for inference in RNA-seq experiments [DOI]
  10. 10 Distribution of miRNA expression across human tissues [DOI]
  11. 11 Deciphering miRNAs’ Action through miRNA Editing [DOI]
  12. 12 Evolution of microRNA diversity and regulation in animals [DOI]
  13. 13 Animal Models are Essential to Biological Research: Issues and Perspectives [DOI]
  14. 14 Clustered miRNAs and their role in biological functions and diseases [DOI]
  15. 15 MiR-145 inhibits cell proliferation of human lung adenocarcinoma by targeting EGFR and NUDT1 [DOI]
  16. 16 Exercise adaptations: molecular mechanisms and potential targets for therapeutic benefit [DOI]
  17. 17 Physical Exercise and Epigenetic Modifications in Skeletal Muscle [DOI]
  18. 18 Circulating miR-155, miR-145 and let-7c as diagnostic biomarkers of the coronary artery disease [DOI]
  19. 19 Changes in vascular and cardiac function after prolonged strenuous exercise in humans [DOI]
  20. 20 Circulating myomiRs: a new class of biomarkers to monitor skeletal muscle in physiology and medicine [DOI]
  21. 21 MicroRNA-143/-145 in Cardiovascular Diseases [DOI]
  22. 22 Acute Exercise Leads to Regulation of Telomere-Associated Genes and MicroRNA Expression in Immune Cells [DOI]
  23. 23 Idiographica: a general-purpose web application to build idiograms on-demand for human, mouse and rat [DOI]
  24. 24 Epigenetic Regulation of microRNAs in Cancer: Shortening the Distance from Bench to Bedside [DOI]
  25. 25 MiR-145: a potential biomarker of cancer migration and invasion.
  26. 26 Circular RNAs and their roles in head and neck cancers [DOI]
  27. 27 Functions and mechanisms of miR-186 in human cancer [DOI]
  28. 28 The role of mTOR in age-related diseases [DOI]
  29. 29 Endurance training: is it bad for you? [DOI]
  30. 30 mTOR and the health benefits of exercise [DOI]
  31. 31 Influence of physical exercise on microRNAs in skeletal muscle regeneration, aging and diseases [DOI]
  32. 32 miR-145 improves metabolic inflammatory disease through multiple pathways [DOI]
  33. 33 Downregulation of miR-31 in Diabetic Nephropathy and its Relationship with Inflammation [DOI]
  34. 34 Strenuous Acute Exercise Induces Slow and Fast Twitch-Dependent NADPH Oxidase Expression in Rat Skeletal Muscle [DOI]
  35. 35 Computational prediction of MicroRNAs targeting GABA receptors and experimental verification of miR-181, miR-216 and miR-203 targets in GABA-A receptor [DOI]
  36. 36 MicroRNAs as Biomarkers of B-cell Lymphoma [DOI]
  37. 37 MicroRNAs in gray and white matter multiple sclerosis lesions: impact on pathophysiology [DOI]
  38. 38 Association of Serum miR-186-5p With the Prognosis of Acute Coronary Syndrome Patients After Percutaneous Coronary Intervention [DOI]
  39. 39 Prioritizing Disease Candidate Proteins in Cardiomyopathy-Specific Protein-Protein Interaction Networks Based on “Guilt by Association” Analysis [DOI]
  40. 40 Inhibition of miR‑186‑5p contributes to high glucose‑induced injury in AC16 cardiomyocytes [DOI]
  41. 41 miR‐186 modulates hepatocellular carcinoma cell proliferation and mobility via targeting MCRS1‐mediated Wnt/β‐catenin signaling [DOI]
  42. 42 Comparative Analysis of Skeletal Muscle Transcriptional Signatures Associated With Aerobic Exercise Capacity or Response to Training in Humans and Rats [DOI]
  43. 43 Orthology for comparative genomics in the mouse genome database [DOI]
  44. 44 Rat models for low and high adaptive response to exercise differ for stress‐related memory and anxiety [DOI]
  45. 45 A compendium of physical exercise-related human genes: an ’omic scale analysis [DOI]
  46. 46 Transcriptional profile in rat muscle: down-regulation networks in acute strenuous exercise [DOI]
  47. 47 Perspective: machines for RNAi [DOI]

Cited by 0

No indexed citations yet.

Related research

Article metrics

Real usage data collected on this platform.

0

Page views

0

PDF downloads

0

Outbound clicks

0

Citations

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

No views recorded yet.

Traffic sources

Referring site, by host.

No traffic recorded yet.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.