Pharmacological Investigation of In-vitro Anti-inflammatory, Antimicrobial, Thrombolytic, Cytotoxic and In vivo Analgesic Activities of Ethanolic Leaf Extract of Diospyros malabarica
Maria Chowdhury, Lamia Akter Sultana, Azijun Chowdhury Joya, Hasib Khan Shomudro
Journal of Advances in Medical and Pharmaceutical Sciences · pp. 1–11 · Published 28 Aug 2023
10.9734/jamps/2023/v25i8630Abstract
Aims: This study was designed to investigate the effects of leaf extracts of Diospyros malabarica plant (EEDML) on a number of in vitro activities, including antibacterial, anti-inflammatory, cytotoxic, and thrombolytic activities, as well as in vivo analgesic activities. Place and Duration of Study: The Laboratory of Phytochemistry and Pharmacology at the Department of Pharmacy and the Laboratory of Microbiology at Stamford University Bangladesh and the Bangladesh Council of Scientific and Industrial Research, Dhaka, conducted this investigation from January 2022 to June 2023. Methodology: The study employed the Egg Albumin Protein Denaturation Assay to evaluate the anti-inflammatory effects of various doses. The Disk Diffusion Method was employed for conducting an antimicrobial assay. Different tests were conducted to evaluate the effects of thrombolytic and cytotoxic substances. These tests included the Clot Lysis assay and the Brine Shrimp Lethality Assay. In-vivo analgesic testing was conducted using the hot plate method and the acetic acid-induced writhing test. Results: The experimental tests showed significant pain-relieving effects, with inhibition percentages of 46.2% and 66.61% observed at doses of 200 and 400 mg/kg, respectively. The anti-inflammatory test showed a significant inhibition of EEDML at a concentration of 1000μg/mL, with an inhibition rate of 85.45%. The antimicrobial test showed a moderate antimicrobial effect, with a zone of inhibition ranging from 07-15, depending on the microorganism and dosage. The thrombolytic test showed a clot lysis rate of 85.37%, while the cytotoxic test indicated an LC50 value of 1.47. Conclusion: EEDML's phytochemical displays strong pharmacological properties, suggesting its potential for drug discovery in diverse areas.
References (13)
- 1 Natural products: A continuing source of novel drug leads [DOI]
- 2 In vitro antimicrobial activity of ethanol and water extracts of Cassia alata [DOI]
- 3 Herbal Drugs: Ethnomedicine to Modern Medicine [DOI]
- 4 Identification of bioactive molecule from Withania somnifera (Ashwagandha) as SARS-CoV-2 main protease inhibitor [DOI]
- 5 Anti-inflammatory and analgesic properties of Moroccan medicinal plants: Phytochemistry, in vitro and in vivo investigations, mechanism insights, clinical evidences and perspectives [DOI]
- 6 Zebrafish: A Model Deciphering the Impact of Flavonoids on Neurodegenerative Disorders [DOI]
- 7 Antibacterial and cytotoxic activity of Brazilian plant extracts - Clusiaceae [DOI]
- 8 Anti-inflammatory and analgesic activity based on polymorphism of cedrol in mice [DOI]
- 9 Diospyros malabarica Fruit Extract Derived Silver Nanoparticles: A Biocompatible Antibacterial Agent [DOI]
- 10 In-vitro and In-vivo Pharmacological Evaluation of Persicaria lapathifolia Available in Bangladesh [DOI]
- 11 Screening of Ethanolic Extract of Diospyros malabarica Desr. Bark for Anti-diabetic and Antioxidant Potential. [DOI]
- 12 In-Vitro Antioxidant, Anti-Arthritis, Anti-inflammatory, Thrombolysis, Anti-bacterial, and in-Vivo Neuropharmacological Activities of Bioactive Metabolites of Solanum americanum Mill. [DOI]
- 13 Review Article: Review Article [DOI]
Cited by 8
Maria Chowdhury, Tajmim Jahan Ritu, Nazmun Nahar · Journal of Applied Life Sciences International · 2024
Sheikh Hasibur Rahman, Md. Shafiqul Islam, Kazi Nadim Hossain · Pharmacological Research - Natural Products · 2025
Aishwarya Jain, Kiran Bhise · Drug Development and Industrial Pharmacy · 2025
Aimé G. Fankam, Vaderament-A. Nchiozem-Ngnitedem, Armel J. Seukep · Journal of Ethnopharmacology · 2026
Showing 4 of 8 known citations — external sources report more than can currently be individually listed.
Related research
- Review: Parkia speciosa as Valuable, Miracle of Nature — shares topic coverage
- Urinary Pathogens amongst Parturients at the University of Port Harcourt Teaching Hospital, South-South, Nigeria — shares topic coverage
- Antimicrobial Studies on Leaf and Stem Extracts of Solanum erianthum — shares topic coverage
- An in-vitro Analysis of the Antioxidant and Antimicrobial Properties of the Methanol Extract of Hypoxis hemerocallideacorm (MEHHC) from Botswana — shares topic coverage
- Evaluation of Antimicrobial Potency and Phytochemical Screening of Persea americana Leaf Extracts against Selected Bacterial and Fungal Isolates of Clinical Importance — shares topic coverage
Article metrics
Real usage data collected on this platform.
59
Page views
0
PDF downloads
19
Outbound clicks
8
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.