Skip to content
Research Article Open access CC BY 4.0

Phylogenetic Relationship of Some Species of Genus Junonia Butterfly Using RAPD

Pallavi V. Singh

Asian Journal of Research in Zoology · pp. 37–43 · Published 22 Feb 2023

10.9734/ajriz/2023/v6i1105

Abstract

The present investigation was carried out to find out similarities and diversities between four species of the genus Junonia belonging to Nymphalidae, the largest family of butterflies. The genomic DNA eluded from the species, J. lemonias, J. alamana, J. hierta and J. orithiya were screened by nine universal primers OPA-1, OPA-2, OPA-3, OPA-4, OPA-5, OPP-9, OPP-18, OPN-17, OPN-16 through RAPD - PCR. Primer OPN-17 did not produce any bands, whereas, the discrete banding pattern was observed in the remaining 8 primers. Primer OPP-18 distinctly highlights only species-specific bands ranging below 200 base pairs. Primer OPA-2 that produced significant banding patterns was taken into consideration for further analysis. The dendrogram was constructed by using binary data interpreted from the RAPD gel image. Two clades were obtained, where in one clade taxa J. hierta and J. lemonias cluster as sister taxa, and in the other clade taxa J. alamana and J. orithiya cluster as sister taxa. The investigation was supported by the Jaccard similarity and distance indices matrix and Principal component analysis. The interpretation from obtained dendrogram and distance matrix reveals that there is closeness within two species in their genetic makeup, whereas some genetic characters are expressed as species-specific. Henceforth, these patterns produced by respective primers can be considered as diagnostic bands and may contribute to molecular markers for Junonia species identification. Thus, in related evolutionary studies whenever be under investigation these markers will play a pivotal role in concluding the direction of evolution.

Dendrogram DNA Junonia molecular marker PCA polymorphism RAPD-PCR

References (14)

  1. 1 MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods [DOI]
  2. 2 DNA polymorphisms amplified by arbitrary primers are useful as genetic markers [DOI]
  3. 3 Evolution of Protein Molecules [DOI]
  4. 4 The Unusual Origin of the Polymerase Chain Reaction [DOI]
  5. 5 Butterflies of the Indian Region
  6. 6 The book of Indian butterflies
  7. 7 The identification of Indian butterflies [DOI]
  8. 8 Principal Component Analysis [DOI]
  9. 9 Ecology of tropical butterflies in rainforest gaps [DOI]
  10. 10 THE ROLE OF NECTAR SOURCE DISTRIBUTION IN HABITAT USE AND OVIPOSITION BY THE TIGER SWALLOWTAIL BUTTERFLY [DOI]
  11. 11 Ecology and conservation needs of nymphalid butterflies in disturbed tropical forest of eastern Himalayan biodiversity hotspot, Assam, India [DOI]
  12. 12 Phylogenetic relationship and morphological evolution in the subfamily Limenitidinae (Lepidoptera: Nymphalidae) [DOI]
  13. 13 RAPD markers system as a useful tool for rapid identification of the origin of lizard contaminants in food [DOI]
  14. 14 Butterfly Species Richness in Selected West Albertine Rift Forests [DOI]

Cited by 0

No indexed citations yet.

Related research

Article metrics

Real usage data collected on this platform.

12

Page views

0

PDF downloads

8

Outbound clicks

0

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.