Phytochemical Characterization and Radical Scavenging Activity of Aqueous Extracts of Medicinal Plants from Portugal
Ana F. Vinha, Marta O. Soares, Ana Castro, António Santos, Maria Beatriz P.P. Oliveira, Marisa Machado
European Journal of Medicinal Plants · pp. 335–347 · Published 12 Nov 2012
10.9734/EJMP/2012/1946Abstract
Aims: The aim of this study was to evaluate the total phenolic and flavonoid contents of four medicinal plants from Portugal usually consumed as tea infusion, Melissa officinalis (Lamiaceae) – lemon balm, Matricaria chamomilla (Asteraceae) – chamomile, Olea europaea (Oleaceae) – olive leaves, and Aloysia triphylla (Verbenaceae) – lemon verbena. Place and Duration of Study: Air-dried leaves of four of the most consumed medicinal plants from Portugal were analyzed in the Department of Pharmacognosy (UFP) and Laboratory of Bromatology (FFUP) and, Department of Pharmacy, and Chemical Laboratory, Health Technology Research Center (CITS) between June 2010 and September 2011. Methodology: Qualitative phytochemical analysis was done for several phytoconstituents (alkaloids, steroids, terpenoids, flavonoids, and tannins). Total phenolic, flavonoid, and tannin contents were quantified by UV-Vis spectrophotometry. It was also analyzed the possible correlation between antioxidant activity (in vitro) and the synergistic effect between different phytochemicals, using the free radical 2,2’-diphenyl-1-picrylhydrazyl (DPPH•). Results: Steroids, terpenoids, flavonoids and tannin, were present in Melissa officinalis and Matricaria chamomilla. In Olea europaea leaves, all the chemical constituents were present except alkaloids, and terpenoids. Aloysia triphylla showed the presence of all the constituents. Total phenol contents ranged from 12.91mg.100g-1 to 87.25 mg.100g-1 and flavonoid contents ranged from 25.17mg.100g-1 to 57.28mg.100g-1. The screening of the leaf of the four selected medicinal plants indicates that the presence of high phenolic content may be due to the presence of tannins and flavonoids which are known to possess antioxidant activities. A slight correlation has been observed between total phenolics and antioxidant activity. Conclusion: Our findings provided evidence that aqueous extracts of these tested plants from Portugal contain medicinally important bioactive compounds. Results showed that plants from Portugal usually used as tea infusions are a good source of phytochemical compounds presenting antioxidant activity, so their consumption must be incremented in younger generations which usually consume other less beneficial drinks.
References (43)
- 1 Use of a free radical method to evaluate antioxidant activity [DOI]
- 2 Free radicals and antioxidants in normal physiological functions and human disease [DOI]
- 3 The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals [DOI]
- 4 Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis
- 5 Plant Phenolics: Extraction, Analysis and Their Antioxidant and Anticancer Properties [DOI]
- 6 Antioxidant Activity and Total Phenolics in Selected Fruits, Vegetables, and Grain Products [DOI]
- 7 Medicinal plants and traditional medicine in Africa
- 8 Screening of radical scavenging activity of some medicinal and aromatic plant extracts [DOI]
- 9 Screening of Plant Extracts for Antioxidant Activity: a Comparative Study on Three Testing Methods [DOI]
- 10 Screening of 70 medicinal plant extracts for antioxidant capacity and total phenols [DOI]
- 11 Dietary Polyphenols and Their Biological Significance [DOI]
- 12 Antioxidant activity and total phenolic content of Iranian Ocimum accessions [DOI]
- 13 Antimicrobial and Antioxidant Properties of Rosemary and Sage (Rosmarinus officinalis L. and Salvia officinalis L., Lamiaceae) Essential Oils [DOI]
- 14 Lippia: traditional uses, chemistry and pharmacology: a review [DOI]
- 15 Olive tree ( Olea europaea ) leaves: potential beneficial effects on human health [DOI]
- 16 Bacterial lipid composition and the antimicrobial efficacy of cationic steroid compounds (Ceragenins) [DOI]
- 17 Phenolic profiles of Portuguese olive fruits (Olea europaea L.): Influences of cultivar and geographical origin [DOI]
- 18 Pharmacological profile of apigenin, a flavonoid isolated from Matricaria chamomilla [DOI]
- 19 Comparison of polyphenolic composition and antioxidant activity of wild Italian blueberries and some cultivated varieties [DOI]
- 20 Screening of some medicinal plants of Ethiopia for their anti-microbial properties and chemical profiles [DOI]
- 21 Solvent extraction study of antioxidants from Balm (Melissa officinalis L.) leaves [DOI]
- 22 Determination of polyphenolic compounds in commercial herbal drugs and spices from Lamiaceae: thyme, wild thyme and sweet marjoram by chromatographic techniques [DOI]
- 23 Evaluation of antioxidant potential of ethyl acetate extract/fractions of Acacia auriculiformis A. Cunn [DOI]
- 24 Antimicrobial properties of grape seed extracts and their effectiveness after incorporation into pea starch films [DOI]
- 25 Flavonoids, Vascular Function and Cardiovascular Protection [DOI]
- 26 Optimization of extraction process for phenolic acids from black cohosh (Cimicifuga racemosa) by pressurized liquid extraction [DOI]
- 27 Free Radicals and Antioxidants: Human and Food System
- 28 Antioxidant Activity of Aqueous Tea Infusions Prepared from Oregano, Thyme and Wild Thyme
- 29 The spasmolytic effect of Aloysia citriodora, Palau (South American cedrón) is partially due to its vitexin but not isovitexin on rat duodenums [DOI]
- 30 ANTIOXIDANT ATTRIBUTES OF FOUR LAMIACEAE ESSENTIAL OILS
- 31 The gastroprotective effect of tannins extracted from duhat (Syzygium cumini Skeels) bark on HCl/ethanol induced gastric mucosal injury in Sprague-Dawley rats.
- 32 Water-soluble polysaccharide from Bupleurum chinense DC: Isolation, structural features and antioxidant activity [DOI]
- 33 Antioxidant activity of supercritical extract of Melissa officinalis subsp. officinalis and Melissa officinalis subsp. inodora [DOI]
- 34 Antimicrobial, antioxidant, and antiviral activities of Retama raetam (Forssk.) Webb flowers growing in Tunisia [DOI]
- 35 Effects of soaking conditions on the antioxidant potentials of oolong tea [DOI]
- 36 Chemical composition and in vitro antimicrobial and antioxidant activities of the essential oil of Nepeta nuda L. subsp. Albiflora (Boiss.) gams [DOI]
- 37 Antidiarrhoeal activity of Geranium incanum Burm. f. (Geraniaceae) leaf aqueous extract in mice [DOI]
- 38 Structure elucidation and antioxidant activity of a novel α-(1→3),(1→4)-d-glucan from Aconitum kusnezoffii Reichb. [DOI]
- 39 Zur Bestimmung der Gerbstoffe mit Casein [DOI]
- 40 Chemical Composition and Antioxidant Activity of Portuguese Diospyrus Kaki Fruit by Geographical Origins [DOI]
- 41 The chemistry of the genus Lippia (Verbenaceae) [DOI]
- 42 Anti oxidant activity of methanolic extracts of female Borassus Flabellifer leaves and roots
- 43 Antioxidants and Beneficial Microvascular Effects [DOI]
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