Plant Tissue Culture Regeneration and Aseptic Techniques
E. E. Ikenganyia, M. A. N. Anikwe, T. E. Omeje, J. O. Adinde
Asian Journal of Biotechnology and Bioresource Technology · pp. 1–6 · Published 7 Sep 2017
10.9734/AJB2T/2017/31724Abstract
The science or art of growing plant cells, tissues or organs isolated from the mother plant on artificial media is known as plant tissue culture. The merits of plant tissue culture in crop production is that it helps in disease elimination, quick international distribution of genetic resources, germplasm conservation and reduction in quarantine requirements, time and space of regeneration. This paper looks at the materials for in vitro plant tissue culture, different methods of plant tissue culture media preparations, protocols of hygiene and sterilization in plant tissue culture and techniques in plant tissue culture for crop improvement.
References (9)
- 1 A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures [DOI]
- 2 Plant tissue culture.
- 3 Genetic integrity of cryopreserved plant cells: a review.
- 4 Totipotency and embryogenesis in plant cell and tissue cultures [DOI]
- 5 Production of somatic hybrid plants, ‘Brassicomoricandia’, through protoplast fusion between Moricandia arvensis and Brassica oleracea [DOI]
- 6 Protoplast Fusion for Production of Hybrid Plants between Citrus and Its Related Genera [DOI]
- 7 STUDY OF INTER-GENERIC HYBRIDIZATION POSSIBILITY BETWEEN SALIX AEGYPTICA AND POPULUS CASPICA TO ACHIEVE NEW HYBRIDS [DOI]
- 8 Tissue culture in disease elimination and micropropagation
- 9 Status of plant regeneration of yam.
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