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Preclinical In vivo Acute Toxicity Testing of Hyrdoethanolic Extracts of Ficus thonningii Blume (Moraceae) on Wistar Rat Models

Estella Tembe Fokunang, Ngo Biyiha Marilyn Orlane Ara, Ambassa Pantaleon, Njinkio Nono Borgia Legrand, Ngameni Bathelemy, Fokunang Charles

Journal of Complementary and Alternative Medical Research · pp. 18–34 · Published 24 Apr 2023

10.9734/jocamr/2023/v21i3438

Abstract

Ficus thonningii (Blume) is a medicinal plant whose biological activity has been demonstrated in the management of peptic ulcers. Unfortunately, little scientific data exists on its toxicity. This is why we studied the acute and subacute oral toxicity profile of the hydro-ethanolic extract of the bark of F. thonningii and the systemic exposure of the bioactive components of the plant.Acute toxicity was assessed by administering a single dose of 2000 mg/kg to 5-week-old rats and observed for 14 days. The subacute toxicity study was performed in 6-week-old rats. Animals were orally treated with a daily dose of 125 mg/kg, 250 mg/kg, 500 mg/kg extract for 28 days. A satellite group of animals received 500 mg/kg per day for 28 days and after discontinuation of treatment they were observed for 14 days. Hematological and blood biochemical parameters, as well as kidney and liver histology, were recorded at the end of each experiment. For acute treatment, administration of a dose of 2000 mg/kg did not induce any critical behavioral changes or deaths. Following subacute dosing, biochemical analysis revealed a slight elevation of liver parameters in the 500mg/kg hydroethanolic extract, while no significant increase was observed for kidney parameters. This study showed that daily administration of Ficus thonningii (Blume) for 28 days resulted in the potential to reverse impairment of liver function.

Ficus thonningii Moraceae hydro-ethanolic extract stem bark toxicity Wistar rat

References (20)

  1. 1 Tietz Textbook of Clinical Chemistry and Molecular Diagnostics
  2. 2 Potential toxicity of flavonoids and other dietary phenolics: significance for their chemopreventive and anticancer properties [DOI]
  3. 3 A review on laboratory liver function tests.
  4. 4 Test No. 420: Acute Oral Toxicity - Fixed Dose Procedure [DOI]
  5. 5 Phytochemistry, pharmacology and ethnomedicinal uses of Ficus thonningii (Blume moraceae): A review [DOI]
  6. 6 The Current Medical Diagnosis and Treatment 2009 [DOI]
  7. 7 Dominance and aggression in social groups of male and female rats [DOI]
  8. 8 Biochemical Investigations in the Management of Liver Disease [DOI]
  9. 9 Potential toxicity of flavonoids and other dietary phenolics: significance for their chemopreventive and anticancer properties [DOI]
  10. 10 Abnormalities in measures of liver function and injury in thyroid disorders.
  11. 11 Flavonoids and other bioactive constituents from Ficus thonningii Blume (Moraceae) [DOI]
  12. 12 In VitroandIn VivoAntimalarial Activity ofFicus thonningiiBlume (Moraceae) andLophira alataBanks (Ochnaceae), Identified from the Ethnomedicine of the Nigerian Middle Belt [DOI]
  13. 13 Evaluation of In Vivo Antiulcer Activity of Hydro‐Methanol Extract and Solvent Fractions of the Stem Bark of Ficus thonningii (Moraceae) on Rodent Models [DOI]
  14. 14 Replacing commercial concentrate by Ficus thonningii improved productivity of goats in Ethiopia [DOI]
  15. 15 Alkaline Phosphatases in Biology and Medicine [DOI]
  16. 16 Thonningiiflavanonol A and thonningiiflavanonol B, two novel flavonoids, and other constituents of Ficus thonningii Blume (Moraceae) [DOI]
  17. 17 Enhancement of antibacterial activity through phyto‐fabrication of silver nanoparticles with Ficus thonningii aqueous extracts [DOI]
  18. 18 Ameliorative properties of aqueous extract of Ficus thonningii on erythrocyte osmotic fragility induced by acetaminophen in Rattus norvegicus.
  19. 19 Evaluation of the Toxicity of Secondary Metabolites in Aqueous Extracts of Ficus thonningii Blume in Wistar rats [DOI]
  20. 20 World Health Organization (WHO) [DOI]

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