Preparation, Characterization and Screening of Gold Nanoparticles Using Phenolic Rich Fractions of Amaranthus gangeticus L. for its in-vitro Anti-oxidant, Anti-Diabetic and Anti-Cancer Activities

Abstract

Aim: In this present study, Gold Nanoparticles are prepared by biosynthesis method using Phenolic Rich Fractions of ethanolic leaf extract of Amaranthus gangeticus L. which was characterized and evaluated for their In-vitro antioxidative, antidiabetic, and anti-cancer potential against HeLa cells. Materials and Methods: Plant mediated AuNPs (gold nanoparticles) were synthesized using phenolic rich fractions of ethanolic extract of Amaranthus gangeticus L. and characterization was done by UV–visible spectroscopy, X-ray diffraction (XRD), Fourier Transform infrared (FT-IR) spectroscopy, Scanning Electronic Microscopy (SEM) and TEM analysis and Selected Area Electron Diffraction SAED pattern. The synthesized AuNPs were assessed for its pharmacological studies. Results: Initially the formation of AuNPs has been identified by its change of colour followed by UV–visible spectroscopy (at 550 nm) due to surface Plasmon resonance. Using XRD pattern the crystalline property of AuNPs were confirmed. The functional group existing in Phenolic Rich Fractions of Amaranthus gangeticus L. responsible for the reduction of gold ion and the stabilization of AuNPs was investigated through FT-IR. The spherical shape of AuNPs were studied by SEM and TEM analysis. From the results, it is confirmed that the synthesized AuNPs having good antidiabetic potential at a very low concentration as well as potent anti-cancer activity against the HeLa cancer cells in a dose-dependent manner. It also observed with potent antioxidant activity. Conclusion: From the present study, the synthesized AuNPs using Amaranthus gangeticus L. were found to be very potent to treat the major diseases like cancer and diabetes.