Secondary Metabolite Profiling of the Endophytic Fungus Trichoderma longibrachiatum L2D2 Isolated from Anaphalis contorta
Kistu Singh Nongthombam, Shyamkesho Singh Mutum, Radha Raman Pandey
Annual Research & Review in Biology · pp. 1–15 · Published 8 Feb 2024
10.9734/arrb/2024/v39i230628Abstract
In the last few decades, research on endophytic fungi has been increasing due to its applications in agriculture, medicine, and the pharmaceutical industry. Many studies indicate that fungal endophytes produce various types of bioactive compounds. In this study, the endophytic fungus Trichoderma longibrachiatum L2D2 was isolated from the leaf of the medicinal plant Anaphalis contorta and secondary metabolites were collected. The crude extract was analyzed for the presence of seven phytochemicals, and it was observed that alkaloids, flavonoids, phenolics, steroids, tannins, and terpenoids were produced. It was also found that the endophyte extract had a total flavonoid content of 126.07 (µg of quercetin equivalent/mg) and a total phenolic content of 43.97 (µg of gallic acid equivalent/mg). The Gas Chromatography–Mass Spectrometry (GC-MS) analysis reveals the presence of the major compound Dithiooxamide, which has a retention time (RT) of 50.030%, and the Liquid Chromatography–Mass Spectrometry (LC-MS) study shows the most abundant compounds as Hexanal (RT of 3.687 min) in positive ionization and Rosamultin (RT of 24.387 min) in negative ionization. Fourier-Transform Infrared (FTIR) Spectroscopy analysis also shows the presence of alcohol, aldehyde, aliphatic ether, aliphatic primary amine, alkene, alkyl halide, amine, aromatic amine, aromatic compound, carboxylic acid, ketone, phenol, and sulfoxide as functional groups. Further studies, like the purification of bioactive compounds and their biological activities, will reveal the applicability of T. longibrachiatum L2D2 in industries.
References (58)
- 1 Antimicrobial resistance: a global multifaceted phenomenon [DOI]
- 2 Bioactive Microbial Metabolites [DOI]
- 3 How to Read and Interpret FTIR Spectroscope of Organic Material [DOI]
- 4 ITS as an environmental DNA barcode for fungi: an in silico approach reveals potential PCR biases [DOI]
- 5 Multifaceted Interactions Between Endophytes and Plant: Developments and Prospects [DOI]
- 6 Advances in Fourier transform infrared (FTIR) spectroscopy of biological tissues [DOI]
- 7 Fungal endophytes as prolific source of phytochemicals and other bioactive natural products: A review [DOI]
- 8 The Use of Endophytes to Obtain Bioactive Compounds and Their Application in Biotransformation Process [DOI]
- 9 Defensive mutualism between plants and endophytic fungi? [DOI]
- 10 Terpenoids [DOI]
- 11 Untapped Potentials of Endophytic Fungi: A Review of Novel Bioactive Compounds with Biological Applications [DOI]
- 12 Extraction and identification of bioactive compounds (eicosane and dibutyl phthalate) produced by Streptomyces strain KX852460 for the biological control of Rhizoctonia solani AG-3 strain KX852461 to control target spot disease in tobacco leaf [DOI]
- 13 Evaluation of bioactive secondary metabolites from endophytic fungus Pestalotiopsis neglecta BAB-5510 isolated from leaves of Cupressus torulosa D.Don [DOI]
- 14 Isolation Procedures for Endophytic Microorganisms [DOI]
- 15 Plant-microbe interactions in the phyllosphere: facing challenges of the anthropocene [DOI]
- 16 Diterpenes from the berries of Juniperus excelsa [DOI]
- 17 Benserazide, a dopadecarboxylase inhibitor, suppresses tumor growth by targeting hexokinase 2 [DOI]
- 18 Determination of Antioxidant Activity of Marshmallow Flower ( Althaea officinalis L.) [DOI]
- 19 Isolation and characterization of anticancer flavone chrysin (5,7-dihydroxy flavone)-producing endophytic fungi from Passiflora incarnata L. leaves [DOI]
- 20 Alkaloids [DOI]
- 21 Angularly Prenylated Indole Alkaloids with Antimicrobial and Insecticidal Activities from an Endophytic Fungus Fusarium sambucinum TE-6L [DOI]
- 22 Biological potential and chemical composition of bioactive compounds from endophytic fungi associated with thai mangrove plants [DOI]
- 23 Vincristine and Vinblastine Anticancer Catharanthus Alkaloids: Pharmacological Applications and Strategies for Yield Improvement [DOI]
- 24 Antimicrobial and Phytochemical Screening of Endophytic Fungi Isolated from Spikes of Pinusroxburghii
- 25 Endophytic fungi from medicinal plants: biodiversity and biotechnological applications [DOI]
- 26 Metabolites of Trichoderma longibrachiatum EF5 inhibits soil borne pathogen, Macrophomina phaseolina by triggering amino sugar metabolism [DOI]
- 27 Phytochemical analysis and enzyme analysis of endophytic fungi from Centella asiatica [DOI]
- 28 A novel antibacterial and antifungal phenolic compound from the endophytic fungusPestalotiopsis mangiferae [DOI]
- 29 Biological properties of Endophytic Fungi [DOI]
- 30 Endophytic fungi from Passiflora incarnata: an antioxidant compound source [DOI]
- 31 New Insights into Detection of a Dendrobine Compound From a Novel Endophytic Trichoderma longibrachiatum Strain and Its Toxicity Against Phytopathogenic Bacteria [DOI]
- 32 Recent topics of iridium-catalyzed hydrosilylation of tertiary amides to silylhemiaminals [DOI]
- 33 Hexanal Vapor Induced Resistance against Major Postharvest Pathogens of Banana (Musa acuminata L.) [DOI]
- 34 Novel antimicrobial and antioxidative activity by endophytic Penicillium roqueforti and Trichoderma reesei isolated from Solanum surattense [DOI]
- 35 Bioactive Products from Fungi [DOI]
- 36 Anti-HIV Protease Activity from Rosa Family Plant Extracts and Rosamultin from Rosa rugosa [DOI]
- 37 Tricycloalternarenes A – E: Five New Mixed Terpenoids from the Endophytic Fungal Strain Alternaria alternata Ly83 [DOI]
- 38 Harziane and cadinane terpenoids from the alga-endophytic fungus Trichoderma asperellum A-YMD-9-2 [DOI]
- 39 Synthesis Characterization and Biological Activity Study of New Schiff and Mannich Bases and Some Metal Complexes Derived from Isatin and Dithiooxamide [DOI]
- 40 Rare C25 Steroids Produced by Penicillium chrysogenum P1X, a Fungal Endophyte of Huperzia serrata [DOI]
- 41 Endophytic Mycoflora as a Source of Biotherapeutic Compounds for Disease Treatment [DOI]
- 42 Chemical Composition and Insecticidal Activity of Origanum syriacum L. Essential Oil Against Sitophilus oryzae and Rhyzopertha dominica [DOI]
- 43 Rosamultin from Potentilla anserine L. exhibits nephroprotection and antioxidant activity by regulating the reactive oxygen species/C/EBP homologous protein signaling pathway [DOI]
- 44 Assessing Fungal Biodiversity Using Molecular Markers [DOI]
- 45 Aspergilolide, a steroid lactone produced by an endophytic fungus Aspergillus sp. MBL1612 isolated from Paeonia ostii [DOI]
- 46 Chemical Composition of the Essential Oil of Anaphalis contorta Hook f. [DOI]
- 47 Phytochemical Analysis and Antioxidant Activity Essential Oil of Anaphalis Contorta from Uttrakhand Himalayas [DOI]
- 48 One stain-many compounds (OSMAC) method for production of phenolic compounds using Camarops sp., an endophytic fungus from Alibertia macrophylla (Rubiaceae) [DOI]
- 49 Chapter 12: What does the Anthropocene hold for citizenship? [DOI]
- 50 Plant Secondary Metabolites as a Tool to Investigate Biotic Stress Tolerance in Plants: A Review [DOI]
- 51 A Novel Endophytic Trichoderma longibrachiatum WKA55 With Biologically Active Metabolites for Promoting Germination and Reducing Mycotoxinogenic Fungi of Peanut [DOI]
- 52 LC–MS/MS and GC–MS based phytochemical perspectives and antimicrobial effects of endophytic fungus Chaetomium ovatoascomatis isolated from Euphorbia milii [DOI]
- 53 Cinchonine: A Versatile Pharmacological Agent Derived from Natural Cinchona Alkaloids [DOI]
- 54 Phytochemical Analysis and Antioxidant Activity of Endophytic Fungi Isolated from Dillenia indica Linn. [DOI]
- 55 Phenolic compounds and bioactive extract produced by endophytic fungus Coriolopsis rigida [DOI]
- 56 Ethnomedicinal Plant Use and Practice in Traditional Medicine [DOI]
- 57 A new diketopiperazine-type alkaloid from the endophytic fungus Penicillium expansum [DOI]
- 58 10.13040/ijpsr.0975-8232.11(5).2065-74 [DOI]
Cited by 12
Bishal Pun, Santa Ram Joshi · Systems Microbiology and Biomanufacturing · 2024
Kistu Singh Nongthombam, Surendirakumar Kannaiah, Prabhu Raju · Journal of Inorganic and Organometallic Polymers and Materials · 2024
Baha'uddeen Salisu, Abdullahi Dalhatu Ibrahim, Mukhtar Lawal Gambo · UMYU Scientifica · 2025
Showing 3 of 12 known citations — external sources report more than can currently be individually listed.
Related research
- Effects of the Ethanol Extract of the Stem Bark of Nauclea latifolia Smith [Rubiaceae] on Certain Biochemical and Haematological Indices of Swiss Albino Mice — shares topic coverage
- Antifungal Activities of Azardirachta indica Crude Extracts and Fractions against Strain 1161, P37005 and RM1000 — shares topic coverage
- Phytochemical Analysis of Different Plant Extracts and their Inhibitory Effects on Aspergillus niger and Fusarium solani — shares topic coverage
- Comparative Review of Bioactive Molecules for Antidiabetic Properties — shares topic coverage
- Antimicrobial and Phytochemical Analyses of Extracts of Diplazium sammatii and Pneumatopteris afra on Selected Clinical Strains of Bacteria — shares topic coverage
Article metrics
Real usage data collected on this platform.
46
Page views
0
PDF downloads
4
Outbound clicks
12
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.