Seed bio-priming with Trichoderma harzianum isolates and its Rhizosphere Colonization in Wheat

Abstract

Aims: In this study, the different isolates of Trichoderma harzianum have abilities like antifungal, insecticidal, soil- remediation, plant growth promoting and rhizosphere colonizing properties which assist its biocontrol action were investigated in vitro. Study Design: CRD (Completely Randomized Design). Place and Duration of Study: An experiment was conducted at Plant Pathology Section, College of Agriculture, Nagpur during 2023-2024. Methodology: A seven days sporulated culture of Trichoderma harzianum isolates (ATH, BTH, CTH, DAG, Thdept) and wheat seeds were collected from Plant Pathology Section, College of Agriculture, Nagpur. The best isolate was identified by using biopriming technique on wheat seeds and the root colonization was also observed. Results: Among all the isolates, the best treatment having highest germination per cent, root and shoot length and SVI is T5 (Thdept) i.e. 72.50%, 23.60 cm, 15.97 cm, 2854.50 respectively followed by T2 (BTH). These results showed that two isolates of Trichoderma harzianum could be used as effective biocontrol for promoting the growth of wheat crop in vitro. Conclusion: The growth and yield will increase by adding Trichoderma spp. in soil and make it most resistant wheat crop against different fungus. So by identifying the best isolate or combination of isolates will improve the health and quality of the product.