Structure of the Small Intestinal Mucosa after Acute Hemorrhagic Shock and Reperfusion of the Ischemic Limbs

Abstract

Aims: The purpose of the report is to study Structure of the Small Intestinal Mucosa after Acute Hemorrhagic Shock and Reperfusion of the Ischemic Limbs. Place and Duration of Study: The Department of Histology and Medical Biology, Tashkent State Institute of Dentistry, between March 2013 and March 2015. Methodology and Study Design: Acute hemorrhagic shock was developed by using Wigger modified method. 76 Shinshilla rabbits having 2.6 + 0.2 kg of body weight fasted for 15 hours before they were undergone acute hemorrhagic shock. In one hour the clamps were removed and simultaneously with the blood flow restoration the ischemic limbs were injected with physiologic solution (II), Rheopolyglukin (III; 20 mg/kg), Succinasol (IV; 1.2 mg/kg). The functional system of digestion and absorbtion integrated with the immune system of the small intestional mucosa which normally regulates homeostasis of the inner medium of an organism was defected one hour after reperfusion of the ischemic limbs of rabbits. Results: The basement membrane beneath enterocytes had usual thickness, stroma of villi contained large number of lymphocytes, functionally active eosinophils and mast cells, the plasmatic cells were large in size and contained rough endoplasmic network with dilated profiles. Blood vessels had normal structure. Conclusion: Structural and functional lesions of the supraepithelial layer of mucus in small intestinal mucosa at the acute hemorrhagic shock and reperfusion have led to direct interactions of microorganisms and microvilli of enterocytes, their translocation into the absorbing cells and stroma of villi, phagocytosis and depletion by macrophages, activation of immunocytes and other cells of the connective tissue.  The results indicated the Succinasol had prevented effectively the structural and functional lesions of the small intestinal mucosa and, as a consequence, the interactions of intestinal microorganisms with glycocalix and microvilli and their translocation into the inner medium.