Susceptibility and Resistance Patterns of Bacteria Isolated from Infected Wounds, Burn, Medical Device Tips and Blood to Doxycycline and Septrin from Patients in the City of Benghazi

Abstract

Wound infections and septicemia with drug-resistant bacteria lead to higher mortality and morbidity and increased healthcare costs. We aimed to evaluate the prevalence rate of nosocomial bacterial agents with antimicrobial susceptibility patterns in hospitalized and non-hospitalized patients. Samples are collected from the wound pus, burn; medical device tip and blood of male and female patients and are sent for culture and sensitivity to the Microbiology Department of Al Saleem medical laboratory, Benghazi, Libya, from October 2021 to January 2022. Collected blood samples were directly inoculated into brain heart infusion (BHI) broth, while various swabs were collected by Transport medium and both are cultured on Blood agar, Chocolate agar, MacConkey - aerobic and anaerobic blood agar at 35°C, with CO2. The wound pus was the most samples from which bacteria were isolated, followed by medical device tip swabs, and the least isolation was from a blood specimen. 47 of the specimens were culture-positive. Of these Staph aureus, Strep pneumonia and E. coli were the most abundant bacterial identified in clinical samples. According to susceptibility testing results, antibiotic resistance patterns of Gram-negative bacteria showed that the highest resistance rate was against Septrin (74.4%) and the lowest rate, 17.8%, was related to Doxycycline (44.6%) respectively. The isolates from the nosocomial infection in these patients are resistant to Septrin and Doxycycline antibiotics. We recommend that whenever the diagnosis of nosocomial infection is made, a more effective antibiotic treatment be instituted until the susceptibility of the strain is identified.