Skip to content
Research Article Open access CC BY 3.0

Synthesis of Selenium Nanoparticles by Bacillus laterosporus Using Gamma Radiation

A. I. El-Batal, Tamer M. Essam, Dalia A. El-Zahaby, Magdy A. Amin

Journal of Pharmaceutical Research International · pp. 1364–1386 · Published 27 May 2014

10.9734/BJPR/2014/10412

Abstract

Aim: This study shows the possible synthesis of Selenium Nanoparticles (SeNPs) in aerobic optimized conditions using Bacillus laterosporus (B. laterosporus) bacterial strain. Methodology: B. laterosporus was used to reduce selenium ions (selenite anions) to SeNPs by fermentation in Luria-Bertani Enrichment (EM) medium. Optimization of fermentation conditions using two-level full factorial design was performed. SeNPs were further characterized by UV-Vis., DLS, TEM, FT-IR, EDX and XRD analysis. SeNPs synthesis by Gamma irradiated B. laterosporus cells at different radiation doses was reported. Evaluation the probability of B. laterosporus to synthesis SeNPs by fermentation in skimmed milk aerobically. A microtiterplate assay was used to evaluate the ability of SeNPs to inhibit the biofilm formation of Pseudomonas aeruginosa. Evaluating the antimicrobial activity of some antibiotic agents upon addition of SeNPs was performed. Results: B. laterosporus reduced the soluble, toxic, colorless selenium ions to the insoluble, non-toxic, red elemental SeNPs. Statistical analysis showed that the results were normally distributed. Temperature, incubation period and pH were significant factors in the fermentation process, in which the maximum SeNPs produced (8.37µmole/ml) was at temperature 37ºC, incubation period 48hr, pH7. The Gamma radiation exposure dose 1.5kGy gave the maximum SeNPs produced (10.01 µmole/ml). A pink color appear in the fermented milk revealing the formation of SeNPs-enriched milk. SeNPs inhibit the biofilm formation of Pseudomonas aeruginosa with a percentage reduction of 99.7%. SeNPs increase the antibacterial activity of fucidic acid by 13.6% and 28.5% against Escherichia coli and Staphylococcus aureus respectively. But with Gentamycin sulphate, no change in the antibacterial activity. Conclusion: SeNPs can be synthesized aerobically by the probiotic B. laterosporus bacterial strain. SeNPs can be incorporated in nutraceuticals and functional foods like milk also can be used to inhibit the bacterial biofilm formation and can be added to some antibacterial creams to enhance their antimicrobial activity.

Selenium nanoparticles Bacillus laterosporus Gamma radiation Factorial design Biofilm inhibition Selenium fermented milk

References (29)

  1. 1 Selenium nanoparticles inhibit Staphylococcus aureus growth [DOI]
  2. 2 A microtiter-plate screening method for biofilm disinfection and removal [DOI]
  3. 3 Aerobic biogenesis of selenium nanospheres by Bacillus cereus isolated from coalmine soil [DOI]
  4. 4 Free radical scavenging efficiency of Nano-Se in vitro [DOI]
  5. 5 Extracellular biosynthesis and transformation of selenium nanoparticles and application in H2O2 biosensor [DOI]
  6. 6 Biosynthesis and structural characteristics of selenium nanoparticles by Pseudomonas alcaliphila [DOI]
  7. 7 Glutathione is an essential metabolite required for resistance to oxidative stress in the yeast Saccharomyces cerevisiae [DOI]
  8. 8 Selenium metabolism in Escherichia coli [DOI]
  9. 9 Delayed formation of zero-valent selenium nanoparticles by Bacillus mycoides SeITE01 as a consequence of selenite reduction under aerobic conditions [DOI]
  10. 10 Heavy Metal Tolerance in Stenotrophomonas maltophilia [DOI]
  11. 11 Producing selenium-enriched eggs and meat to improve the selenium status of the general population [DOI]
  12. 12 A microtiter plate assay for Staphylococcus aureus biofilm quantification at various pH levels and hydrogen peroxide supplementation.
  13. 13 Formation of Se (0) Nanoparticles by Duganella sp. and Agrobacterium sp. isolated from Se-laden soil of North-East Punjab, India [DOI]
  14. 14 Isolation and characterization of a fungus for extracellular synthesis of small selenium nanoparticles
  15. 15 Delineation of the molecular basis for selenium-induced growth arrest in human prostate cancer cells by oligonucleotide array.
  16. 16 Biomimetic Synthesis of Selenium Nanospheres by Bacterial Strain JS-11 and Its Role as a Biosensor for Nanotoxicity Assessment: A Novel Se-Bioassay [DOI]
  17. 17 A novel method for the measurement of elemental selenium produced by bacterial reduction of selenite [DOI]
  18. 18 Biosynthesis of metal and oxide nanoparticles using Lactobacilli from yoghurt and probiotic spore tablets [DOI]
  19. 19 Multiple-Stress Tolerance of Ionizing Radiation-Resistant Bacterial Isolates Obtained from Various Habitats: Correlation Between Stresses [DOI]
  20. 20 Gamma radiation mediated green synthesis of gold nanoparticles using fermented soybean-garlic aqueous extract and their antimicrobial activity [DOI]
  21. 21 Aerobic microbial manufacture of nanoscale selenium: exploiting nature’s bio-nanomineralization potential [DOI]
  22. 22 Optimization of microtitre plate assay for the testing of biofilm formation ability in different Salmonella serotypes
  23. 23 Generation of Selenium Containing Nano-Structures By Soil Bacterium, Pseudomonas aeruginosa [DOI]
  24. 24 MICROBIAL SYNTHESIS OF SELINIUM NANOCOMPOSITE USING SACCHAROMYCES CEREVISIAE AND ITS ANTIMICROBIAL ACTIVITY AGAINST PATHOGENS CAUSING NOSOCOMIAL INFECTION
  25. 25 Amelioration of Oxidative Damage Induced in Gamma Irradiated Rats by Nano Selenium and Lovastatin Mixture
  26. 26 Inactivation of Bacillus Spores by Ultraviolet or Gamma Radiation [DOI]
  27. 27 Selenium Toxicity: Aminoacylation and Peptide Bond Formation with Selenomethionine [DOI]
  28. 28 Antifungal activity of biogenic selenium nanoparticles.
  29. 29 Optimization of culture media for Bacillus species by statistical experimental design methods [DOI]

Cited by 30

Enhancement the Mycosynthesis of Selenium Nanoparticles by Using Gamma Radiation

Basma H. Amin, Hanaa Y. Ahmed, Eman M. El Gazzar · Dose-Response · 2021

Biomemetic synthesis of selenium nanoparticles and its biomedical applications

Soumya Menon, Happy Agarwal, S. Venkat Kumar · Green Synthesis, Characterization and Applications of Nanoparticles · 2019

Showing 12 of 30 known citations — external sources report more than can currently be individually listed.

Related research

Article metrics

Real usage data collected on this platform.

33

Page views

0

PDF downloads

3

Outbound clicks

30

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.